Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats

Detalhes bibliográficos
Autor(a) principal: Yousefzadeh,Nasibeh
Data de Publicação: 2016
Outros Autores: Jeddi,Sajad, Alipour,Mohammad Reza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos Brasileiros de Cardiologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2016004100147
Resumo: Abstract Background: Thyroid hormone deficiency during fetal life could affect the cardiac function in later life. The mechanism underlying this action in fetal hypothyroidism (FH) in rats has not been elucidated thus far. Objective: The aim of this study is to evaluation the effect of FH on cardiac function in male rats and to determine the contribution of α-myosin heavy chain (MHC) and β-MHC isoforms. Methods: Six pregnant female rats were randomly divided into two groups: The hypothyroid group received water containing 6-propyl-2-thiouracil during gestation and the controls consumed tap water. The offspring of the rats were tested in adulthood. Hearts from the FH and control rats were isolated and perfused with langendroff setup for measuring hemodynamic parameters; also, the heart mRNA expressions of α- MHC and β-MHC were measured by qPCR. Results: Baseline LVDP (74.0 ± 3.1 vs. 92.5 ± 3.2 mmHg, p < 0.05) and heart rate (217 ± 11 vs. 273 ± 6 beat/min, p < 0.05) were lower in the FH rats than controls. Also, these results showed the same significance in ±dp/dt. In the FH rats, β-MHC expression was higher (201%) and α- MHC expression was lower (47%) than control. Conclusion: Thyroid hormone deficiency during fetal life could attenuate normal cardiac functions in adult rats, an effect at least in part due to the increased expression of β-MHC to α- MHC ratio in the heart.
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spelling Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male RatsHypothyroidismFetusCardiac MyosinsMyocardial ContractionThyroid HormonesRatsAbstract Background: Thyroid hormone deficiency during fetal life could affect the cardiac function in later life. The mechanism underlying this action in fetal hypothyroidism (FH) in rats has not been elucidated thus far. Objective: The aim of this study is to evaluation the effect of FH on cardiac function in male rats and to determine the contribution of α-myosin heavy chain (MHC) and β-MHC isoforms. Methods: Six pregnant female rats were randomly divided into two groups: The hypothyroid group received water containing 6-propyl-2-thiouracil during gestation and the controls consumed tap water. The offspring of the rats were tested in adulthood. Hearts from the FH and control rats were isolated and perfused with langendroff setup for measuring hemodynamic parameters; also, the heart mRNA expressions of α- MHC and β-MHC were measured by qPCR. Results: Baseline LVDP (74.0 ± 3.1 vs. 92.5 ± 3.2 mmHg, p < 0.05) and heart rate (217 ± 11 vs. 273 ± 6 beat/min, p < 0.05) were lower in the FH rats than controls. Also, these results showed the same significance in ±dp/dt. In the FH rats, β-MHC expression was higher (201%) and α- MHC expression was lower (47%) than control. Conclusion: Thyroid hormone deficiency during fetal life could attenuate normal cardiac functions in adult rats, an effect at least in part due to the increased expression of β-MHC to α- MHC ratio in the heart.Sociedade Brasileira de Cardiologia - SBC2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2016004100147Arquivos Brasileiros de Cardiologia v.107 n.2 2016reponame:Arquivos Brasileiros de Cardiologia (Online)instname:Sociedade Brasileira de Cardiologia (SBC)instacron:SBC10.5935/abc.20160099info:eu-repo/semantics/openAccessYousefzadeh,NasibehJeddi,SajadAlipour,Mohammad Rezaeng2016-09-09T00:00:00Zoai:scielo:S0066-782X2016004100147Revistahttp://www.arquivosonline.com.br/https://old.scielo.br/oai/scielo-oai.php||arquivos@cardiol.br1678-41700066-782Xopendoar:2016-09-09T00:00Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)false
dc.title.none.fl_str_mv Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
title Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
spellingShingle Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
Yousefzadeh,Nasibeh
Hypothyroidism
Fetus
Cardiac Myosins
Myocardial Contraction
Thyroid Hormones
Rats
title_short Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
title_full Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
title_fullStr Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
title_full_unstemmed Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
title_sort Effect of Fetal Hypothyroidism on Cardiac Myosin Heavy Chain Expression in Male Rats
author Yousefzadeh,Nasibeh
author_facet Yousefzadeh,Nasibeh
Jeddi,Sajad
Alipour,Mohammad Reza
author_role author
author2 Jeddi,Sajad
Alipour,Mohammad Reza
author2_role author
author
dc.contributor.author.fl_str_mv Yousefzadeh,Nasibeh
Jeddi,Sajad
Alipour,Mohammad Reza
dc.subject.por.fl_str_mv Hypothyroidism
Fetus
Cardiac Myosins
Myocardial Contraction
Thyroid Hormones
Rats
topic Hypothyroidism
Fetus
Cardiac Myosins
Myocardial Contraction
Thyroid Hormones
Rats
description Abstract Background: Thyroid hormone deficiency during fetal life could affect the cardiac function in later life. The mechanism underlying this action in fetal hypothyroidism (FH) in rats has not been elucidated thus far. Objective: The aim of this study is to evaluation the effect of FH on cardiac function in male rats and to determine the contribution of α-myosin heavy chain (MHC) and β-MHC isoforms. Methods: Six pregnant female rats were randomly divided into two groups: The hypothyroid group received water containing 6-propyl-2-thiouracil during gestation and the controls consumed tap water. The offspring of the rats were tested in adulthood. Hearts from the FH and control rats were isolated and perfused with langendroff setup for measuring hemodynamic parameters; also, the heart mRNA expressions of α- MHC and β-MHC were measured by qPCR. Results: Baseline LVDP (74.0 ± 3.1 vs. 92.5 ± 3.2 mmHg, p < 0.05) and heart rate (217 ± 11 vs. 273 ± 6 beat/min, p < 0.05) were lower in the FH rats than controls. Also, these results showed the same significance in ±dp/dt. In the FH rats, β-MHC expression was higher (201%) and α- MHC expression was lower (47%) than control. Conclusion: Thyroid hormone deficiency during fetal life could attenuate normal cardiac functions in adult rats, an effect at least in part due to the increased expression of β-MHC to α- MHC ratio in the heart.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2016004100147
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2016004100147
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/abc.20160099
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
dc.source.none.fl_str_mv Arquivos Brasileiros de Cardiologia v.107 n.2 2016
reponame:Arquivos Brasileiros de Cardiologia (Online)
instname:Sociedade Brasileira de Cardiologia (SBC)
instacron:SBC
instname_str Sociedade Brasileira de Cardiologia (SBC)
instacron_str SBC
institution SBC
reponame_str Arquivos Brasileiros de Cardiologia (Online)
collection Arquivos Brasileiros de Cardiologia (Online)
repository.name.fl_str_mv Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)
repository.mail.fl_str_mv ||arquivos@cardiol.br
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