Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Cardiovascular Surgery (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000200202 |
Resumo: | Introduction: Most cardiomyocytes do not regenerate after myocardial infarction. Porcine small intestinal submucosa has been shown to be effective in tissue repair. Objective: To evaluate myocardial tissue regeneration and functional effects of SIS implantation in pigs after left ventriculotomy. Methods: Fifteen pigs were assigned to two groups: porcine small intestinal submucosa (SIS) (N=10) and control (N=5). The SIS group underwent a mini sternotomy, left ventriculotomy and placement of a SIS patch. The control group underwent a sham procedure. Echocardiography was performed before and 60 days after the surgical procedure. Histological analysis was performed with hematoxylin-eosin stain and markers for actin 1A4, anti sarcomeric actin, connexin43 and factor VIII. Results: Weight gain was similar in both groups. Echocardiography analysis revealed no difference between groups regarding end diastolic and systolic diameters and left ventricular ejection fraction, both pre (P=0.118, P=0.313, P=0.944) and post procedure (P=0.333, P=0.522, P=0.628). Both groups showed an increase in end diastolic (P<0,001 for both) and systolic diameter 60 days after surgery (P=0.005, SIS group and P=0.004, control group). New cardiomyocytes, blood vessels and inflammatory reactions were histologically identified in the SIS group. Conclusion: SIS implantation in pigs after left ventriculotomy was associated with angiomuscular regeneration and no damage in cardiac function. |
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Brazilian Journal of Cardiovascular Surgery (Online) |
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Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricleIntestinal MucosaRegenerationMyocardium Introduction: Most cardiomyocytes do not regenerate after myocardial infarction. Porcine small intestinal submucosa has been shown to be effective in tissue repair. Objective: To evaluate myocardial tissue regeneration and functional effects of SIS implantation in pigs after left ventriculotomy. Methods: Fifteen pigs were assigned to two groups: porcine small intestinal submucosa (SIS) (N=10) and control (N=5). The SIS group underwent a mini sternotomy, left ventriculotomy and placement of a SIS patch. The control group underwent a sham procedure. Echocardiography was performed before and 60 days after the surgical procedure. Histological analysis was performed with hematoxylin-eosin stain and markers for actin 1A4, anti sarcomeric actin, connexin43 and factor VIII. Results: Weight gain was similar in both groups. Echocardiography analysis revealed no difference between groups regarding end diastolic and systolic diameters and left ventricular ejection fraction, both pre (P=0.118, P=0.313, P=0.944) and post procedure (P=0.333, P=0.522, P=0.628). Both groups showed an increase in end diastolic (P<0,001 for both) and systolic diameter 60 days after surgery (P=0.005, SIS group and P=0.004, control group). New cardiomyocytes, blood vessels and inflammatory reactions were histologically identified in the SIS group. Conclusion: SIS implantation in pigs after left ventriculotomy was associated with angiomuscular regeneration and no damage in cardiac function. Sociedade Brasileira de Cirurgia Cardiovascular2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000200202Brazilian Journal of Cardiovascular Surgery v.29 n.2 2014reponame:Brazilian Journal of Cardiovascular Surgery (Online)instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)instacron:SBCCV10.5935/1678-9741.20140070info:eu-repo/semantics/openAccessRamos,Cassiana Maria GarcezFrancisco,Julio CésarOlandoski,MarciaCarvalho,Katherine Athayde Teixeira deCunha,RicardoErbano,Bruna OlandoskiJorge,Lianna FerrariBaena,Cristina PellegrinoAmaral,Vivian Ferreira doNoronha,LuciaMacedo,Rafael Michel deFaria-Neto,José RochaGuarita-Souza,Luiz Césareng2014-08-28T00:00:00Zoai:scielo:S0102-76382014000200202Revistahttp://www.rbccv.org.br/https://old.scielo.br/oai/scielo-oai.php||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br1678-97410102-7638opendoar:2014-08-28T00:00Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)false |
dc.title.none.fl_str_mv |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
title |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
spellingShingle |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle Ramos,Cassiana Maria Garcez Intestinal Mucosa Regeneration Myocardium |
title_short |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
title_full |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
title_fullStr |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
title_full_unstemmed |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
title_sort |
Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle |
author |
Ramos,Cassiana Maria Garcez |
author_facet |
Ramos,Cassiana Maria Garcez Francisco,Julio César Olandoski,Marcia Carvalho,Katherine Athayde Teixeira de Cunha,Ricardo Erbano,Bruna Olandoski Jorge,Lianna Ferrari Baena,Cristina Pellegrino Amaral,Vivian Ferreira do Noronha,Lucia Macedo,Rafael Michel de Faria-Neto,José Rocha Guarita-Souza,Luiz César |
author_role |
author |
author2 |
Francisco,Julio César Olandoski,Marcia Carvalho,Katherine Athayde Teixeira de Cunha,Ricardo Erbano,Bruna Olandoski Jorge,Lianna Ferrari Baena,Cristina Pellegrino Amaral,Vivian Ferreira do Noronha,Lucia Macedo,Rafael Michel de Faria-Neto,José Rocha Guarita-Souza,Luiz César |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramos,Cassiana Maria Garcez Francisco,Julio César Olandoski,Marcia Carvalho,Katherine Athayde Teixeira de Cunha,Ricardo Erbano,Bruna Olandoski Jorge,Lianna Ferrari Baena,Cristina Pellegrino Amaral,Vivian Ferreira do Noronha,Lucia Macedo,Rafael Michel de Faria-Neto,José Rocha Guarita-Souza,Luiz César |
dc.subject.por.fl_str_mv |
Intestinal Mucosa Regeneration Myocardium |
topic |
Intestinal Mucosa Regeneration Myocardium |
description |
Introduction: Most cardiomyocytes do not regenerate after myocardial infarction. Porcine small intestinal submucosa has been shown to be effective in tissue repair. Objective: To evaluate myocardial tissue regeneration and functional effects of SIS implantation in pigs after left ventriculotomy. Methods: Fifteen pigs were assigned to two groups: porcine small intestinal submucosa (SIS) (N=10) and control (N=5). The SIS group underwent a mini sternotomy, left ventriculotomy and placement of a SIS patch. The control group underwent a sham procedure. Echocardiography was performed before and 60 days after the surgical procedure. Histological analysis was performed with hematoxylin-eosin stain and markers for actin 1A4, anti sarcomeric actin, connexin43 and factor VIII. Results: Weight gain was similar in both groups. Echocardiography analysis revealed no difference between groups regarding end diastolic and systolic diameters and left ventricular ejection fraction, both pre (P=0.118, P=0.313, P=0.944) and post procedure (P=0.333, P=0.522, P=0.628). Both groups showed an increase in end diastolic (P<0,001 for both) and systolic diameter 60 days after surgery (P=0.005, SIS group and P=0.004, control group). New cardiomyocytes, blood vessels and inflammatory reactions were histologically identified in the SIS group. Conclusion: SIS implantation in pigs after left ventriculotomy was associated with angiomuscular regeneration and no damage in cardiac function. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000200202 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000200202 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1678-9741.20140070 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Cardiovascular |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Cardiovascular |
dc.source.none.fl_str_mv |
Brazilian Journal of Cardiovascular Surgery v.29 n.2 2014 reponame:Brazilian Journal of Cardiovascular Surgery (Online) instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) instacron:SBCCV |
instname_str |
Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) |
instacron_str |
SBCCV |
institution |
SBCCV |
reponame_str |
Brazilian Journal of Cardiovascular Surgery (Online) |
collection |
Brazilian Journal of Cardiovascular Surgery (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) |
repository.mail.fl_str_mv |
||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br |
_version_ |
1752126598504513536 |