Myocardial regeneration after implantation of porcine small intestinal submucosa in the left ventricle

• Autor(a) principal: Ramos,Cassiana Maria Garcez
• Data de Publicação: 2014
• Outros Autores: Francisco,Julio César, Olandoski,Marcia, Carvalho,Katherine Athayde Teixeira de, Cunha,Ricardo, Erbano,Bruna Olandoski, Jorge,Lianna Ferrari, Baena,Cristina Pellegrino, Amaral,Vivian Ferreira do, Noronha,Lucia, Macedo,Rafael Michel de, Faria-Neto,José Rocha, Guarita-Souza,Luiz César
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Cardiovascular Surgery (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382014000200202
• topic: Intestinal Mucosa; Regeneration; Myocardium

Introduction: Most cardiomyocytes do not regenerate after myocardial infarction. Porcine small intestinal submucosa has been shown to be effective in tissue repair. Objective: To evaluate myocardial tissue regeneration and functional effects of SIS implantation in pigs after left ventriculotomy. Methods: Fifteen pigs were assigned to two groups: porcine small intestinal submucosa (SIS) (N=10) and control (N=5). The SIS group underwent a mini sternotomy, left ventriculotomy and placement of a SIS patch. The control group underwent a sham procedure. Echocardiography was performed before and 60 days after the surgical procedure. Histological analysis was performed with hematoxylin-eosin stain and markers for actin 1A4, anti sarcomeric actin, connexin43 and factor VIII. Results: Weight gain was similar in both groups. Echocardiography analysis revealed no difference between groups regarding end diastolic and systolic diameters and left ventricular ejection fraction, both pre (P=0.118, P=0.313, P=0.944) and post procedure (P=0.333, P=0.522, P=0.628). Both groups showed an increase in end diastolic (P<0,001 for both) and systolic diameter 60 days after surgery (P=0.005, SIS group and P=0.004, control group). New cardiomyocytes, blood vessels and inflammatory reactions were histologically identified in the SIS group. Conclusion: SIS implantation in pigs after left ventriculotomy was associated with angiomuscular regeneration and no damage in cardiac function.