Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Cardiovascular Surgery (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200005 |
Resumo: | AbstractIntroduction:Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel.Methods:Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody.Results:The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody.Conclusion:Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. |
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Brazilian Journal of Cardiovascular Surgery (Online) |
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|
spelling |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibodyCoronary Artery DiseaseDrug-Eluting StentsCoronary RestenosisVascular Endothelial Growth FactorAntigens, CD34AbstractIntroduction:Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel.Methods:Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody.Results:The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody.Conclusion:Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy.Sociedade Brasileira de Cirurgia Cardiovascular2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200005Brazilian Journal of Cardiovascular Surgery v.30 n.2 2015reponame:Brazilian Journal of Cardiovascular Surgery (Online)instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)instacron:SBCCV10.5935/1678-9741.20150016info:eu-repo/semantics/openAccessSong,Chun-LiLi,QianYu,Yun-PengWang,GuanWang,Jin-PengLu,YangZhang,Ji-ChangDiao,Hong-YingLiu,Jian-GenLiu,Yi-HangLiu,JiaLi,YingCai,DanLiu,Bineng2015-10-09T00:00:00Zoai:scielo:S0102-76382015000200005Revistahttp://www.rbccv.org.br/https://old.scielo.br/oai/scielo-oai.php||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br1678-97410102-7638opendoar:2015-10-09T00:00Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)false |
dc.title.none.fl_str_mv |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
title |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
spellingShingle |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody Song,Chun-Li Coronary Artery Disease Drug-Eluting Stents Coronary Restenosis Vascular Endothelial Growth Factor Antigens, CD34 |
title_short |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
title_full |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
title_fullStr |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
title_full_unstemmed |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
title_sort |
Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |
author |
Song,Chun-Li |
author_facet |
Song,Chun-Li Li,Qian Yu,Yun-Peng Wang,Guan Wang,Jin-Peng Lu,Yang Zhang,Ji-Chang Diao,Hong-Ying Liu,Jian-Gen Liu,Yi-Hang Liu,Jia Li,Ying Cai,Dan Liu,Bin |
author_role |
author |
author2 |
Li,Qian Yu,Yun-Peng Wang,Guan Wang,Jin-Peng Lu,Yang Zhang,Ji-Chang Diao,Hong-Ying Liu,Jian-Gen Liu,Yi-Hang Liu,Jia Li,Ying Cai,Dan Liu,Bin |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Song,Chun-Li Li,Qian Yu,Yun-Peng Wang,Guan Wang,Jin-Peng Lu,Yang Zhang,Ji-Chang Diao,Hong-Ying Liu,Jian-Gen Liu,Yi-Hang Liu,Jia Li,Ying Cai,Dan Liu,Bin |
dc.subject.por.fl_str_mv |
Coronary Artery Disease Drug-Eluting Stents Coronary Restenosis Vascular Endothelial Growth Factor Antigens, CD34 |
topic |
Coronary Artery Disease Drug-Eluting Stents Coronary Restenosis Vascular Endothelial Growth Factor Antigens, CD34 |
description |
AbstractIntroduction:Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel.Methods:Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody.Results:The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody.Conclusion:Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382015000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1678-9741.20150016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Cardiovascular |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Cardiovascular |
dc.source.none.fl_str_mv |
Brazilian Journal of Cardiovascular Surgery v.30 n.2 2015 reponame:Brazilian Journal of Cardiovascular Surgery (Online) instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) instacron:SBCCV |
instname_str |
Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) |
instacron_str |
SBCCV |
institution |
SBCCV |
reponame_str |
Brazilian Journal of Cardiovascular Surgery (Online) |
collection |
Brazilian Journal of Cardiovascular Surgery (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) |
repository.mail.fl_str_mv |
||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br |
_version_ |
1752126598927089664 |