Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease

Detalhes bibliográficos
Autor(a) principal: Leal,Raquel Franco
Data de Publicação: 2013
Outros Autores: Milanski,Marciane, Coy,Cláudio Saddy Rodrigues, Portovedo,Mariana, Rodrigues,Viviane Soares, Coope,Andressa, Ayrizono,Maria de Lourdes Setsuko, Fagundes,João José, Velloso,Lício Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of Coloproctology (Rio de Janeiro. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632013000100009
Resumo: BACKGROUND: Recently, mesenteric fat has been proposed to play a role in the pathophysiology of Crohn's disease (CD), as fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and creeping fat tissue in CD. OBJECTIVE: Evaluate autophagy-related proteins and proinflammatory cytokines in intestinal mucosa and mesenteric fat in patients with CD and controls. PATIENTS AND METHODS: Ten patients with CD, eight with non-inflammatory disease who underwent surgery, and eight with normal ileocolonoscopy were studied. The expression of LC3-II, TNF-α and IL-23 was determined by immunoblot of protein extracts. In addition, total RNA of LC3 and Atg16-L1 were determined using RT-PCR. RESULTS: The expression of LC3-II was significantly lower in the mesenteric tissue of CD when compared to controls (p < 0.05). In contrast, the intestinal mucosa of the CD group had higher levels of LC3-II (p < 0.05). However, mRNA expression of autophagy-related proteins was similar when compared to mesenteric fat groups. TNF-α and IL-23 expressions were higher in intestinal mucosa of CD than in control (p < 0.05). CONCLUSION: These findings suggest a defect in the autophagic activity of the creeping fat tissue in CD, which could be involved with the maintenance of the inflammatory process in the intestinal mucosa.
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spelling Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's diseaseCrohn's diseaseInflammatory bowel diseasesAutophagyCytokinesMesenteric fat BACKGROUND: Recently, mesenteric fat has been proposed to play a role in the pathophysiology of Crohn's disease (CD), as fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and creeping fat tissue in CD. OBJECTIVE: Evaluate autophagy-related proteins and proinflammatory cytokines in intestinal mucosa and mesenteric fat in patients with CD and controls. PATIENTS AND METHODS: Ten patients with CD, eight with non-inflammatory disease who underwent surgery, and eight with normal ileocolonoscopy were studied. The expression of LC3-II, TNF-α and IL-23 was determined by immunoblot of protein extracts. In addition, total RNA of LC3 and Atg16-L1 were determined using RT-PCR. RESULTS: The expression of LC3-II was significantly lower in the mesenteric tissue of CD when compared to controls (p < 0.05). In contrast, the intestinal mucosa of the CD group had higher levels of LC3-II (p < 0.05). However, mRNA expression of autophagy-related proteins was similar when compared to mesenteric fat groups. TNF-α and IL-23 expressions were higher in intestinal mucosa of CD than in control (p < 0.05). CONCLUSION: These findings suggest a defect in the autophagic activity of the creeping fat tissue in CD, which could be involved with the maintenance of the inflammatory process in the intestinal mucosa. Sociedade Brasileira de Coloproctologia2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632013000100009Journal of Coloproctology (Rio de Janeiro) v.33 n.1 2013reponame:Journal of Coloproctology (Rio de Janeiro. Online)instname:Sociedade Brasileira de Coloproctologia (SBCP)instacron:SBCP10.1590/S2237-93632013000100003info:eu-repo/semantics/openAccessLeal,Raquel FrancoMilanski,MarcianeCoy,Cláudio Saddy RodriguesPortovedo,MarianaRodrigues,Viviane SoaresCoope,AndressaAyrizono,Maria de Lourdes SetsukoFagundes,João JoséVelloso,Lício Augustoeng2015-07-24T00:00:00Zoai:scielo:S2237-93632013000100009Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2237-9363&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcp@sbcp.org.br2317-64232237-9363opendoar:2015-07-24T00:00Journal of Coloproctology (Rio de Janeiro. Online) - Sociedade Brasileira de Coloproctologia (SBCP)false
dc.title.none.fl_str_mv Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
title Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
spellingShingle Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
Leal,Raquel Franco
Crohn's disease
Inflammatory bowel diseases
Autophagy
Cytokines
Mesenteric fat
title_short Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
title_full Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
title_fullStr Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
title_full_unstemmed Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
title_sort Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
author Leal,Raquel Franco
author_facet Leal,Raquel Franco
Milanski,Marciane
Coy,Cláudio Saddy Rodrigues
Portovedo,Mariana
Rodrigues,Viviane Soares
Coope,Andressa
Ayrizono,Maria de Lourdes Setsuko
Fagundes,João José
Velloso,Lício Augusto
author_role author
author2 Milanski,Marciane
Coy,Cláudio Saddy Rodrigues
Portovedo,Mariana
Rodrigues,Viviane Soares
Coope,Andressa
Ayrizono,Maria de Lourdes Setsuko
Fagundes,João José
Velloso,Lício Augusto
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leal,Raquel Franco
Milanski,Marciane
Coy,Cláudio Saddy Rodrigues
Portovedo,Mariana
Rodrigues,Viviane Soares
Coope,Andressa
Ayrizono,Maria de Lourdes Setsuko
Fagundes,João José
Velloso,Lício Augusto
dc.subject.por.fl_str_mv Crohn's disease
Inflammatory bowel diseases
Autophagy
Cytokines
Mesenteric fat
topic Crohn's disease
Inflammatory bowel diseases
Autophagy
Cytokines
Mesenteric fat
description BACKGROUND: Recently, mesenteric fat has been proposed to play a role in the pathophysiology of Crohn's disease (CD), as fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and creeping fat tissue in CD. OBJECTIVE: Evaluate autophagy-related proteins and proinflammatory cytokines in intestinal mucosa and mesenteric fat in patients with CD and controls. PATIENTS AND METHODS: Ten patients with CD, eight with non-inflammatory disease who underwent surgery, and eight with normal ileocolonoscopy were studied. The expression of LC3-II, TNF-α and IL-23 was determined by immunoblot of protein extracts. In addition, total RNA of LC3 and Atg16-L1 were determined using RT-PCR. RESULTS: The expression of LC3-II was significantly lower in the mesenteric tissue of CD when compared to controls (p < 0.05). In contrast, the intestinal mucosa of the CD group had higher levels of LC3-II (p < 0.05). However, mRNA expression of autophagy-related proteins was similar when compared to mesenteric fat groups. TNF-α and IL-23 expressions were higher in intestinal mucosa of CD than in control (p < 0.05). CONCLUSION: These findings suggest a defect in the autophagic activity of the creeping fat tissue in CD, which could be involved with the maintenance of the inflammatory process in the intestinal mucosa.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632013000100009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632013000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S2237-93632013000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Coloproctologia
publisher.none.fl_str_mv Sociedade Brasileira de Coloproctologia
dc.source.none.fl_str_mv Journal of Coloproctology (Rio de Janeiro) v.33 n.1 2013
reponame:Journal of Coloproctology (Rio de Janeiro. Online)
instname:Sociedade Brasileira de Coloproctologia (SBCP)
instacron:SBCP
instname_str Sociedade Brasileira de Coloproctologia (SBCP)
instacron_str SBCP
institution SBCP
reponame_str Journal of Coloproctology (Rio de Janeiro. Online)
collection Journal of Coloproctology (Rio de Janeiro. Online)
repository.name.fl_str_mv Journal of Coloproctology (Rio de Janeiro. Online) - Sociedade Brasileira de Coloproctologia (SBCP)
repository.mail.fl_str_mv ||sbcp@sbcp.org.br
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