Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Planta daninha (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000300020 |
Resumo: | The action of herbicides that affect the integrity of cell membranes and cause leakage, like PPO-inhibitors, can be detected by measuring the electric conductivity (EC) of a solution in which the plant tissue target is incubated in the presence of herbicide. The objectives of this work were to confirm PPO resistance in a new Euphorbia heterophylla (EPHHL) biotype, and to compare the electrolyte leakage from R and S to PPO-inhibitors biotypes, using two different methods of incubation in a solution containing herbicides. One experiment was carried in greenhouse and three in laboratory, with a completely randomized design. In the greenhouse experiment, four biotypes of EPHHL were sprayed with seven rates of fomesafen to confirm resistance in suspected biotypes. Leaf disks from R and S EPHHL biotypes in the second and the third experiments and entire leaves in the fourth experiment were incubated in a solution containing PPO-inhibitors to subsequently determine EC of solution. The study confirmed the resistance to PPO-inhibitors in two EPHHL biotypes. There were no significant differences between S and R biotypes in the experiments with the incubation of leaf disks, but incubation of entire leaves of EPHHL S biotype showed higher EC when in a solution with fomesafen, in comparison to the R biotype. The results of this work are an indirect evidence that resistance to PPO-inhibitors is related to lower absorption of herbicide by the shoots and also to some kind of mechanism to cope with oxidative stress. |
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Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicidescell disruptionelectrical conductivityherbicide absorptionresistance mechanismwild poinsettiaThe action of herbicides that affect the integrity of cell membranes and cause leakage, like PPO-inhibitors, can be detected by measuring the electric conductivity (EC) of a solution in which the plant tissue target is incubated in the presence of herbicide. The objectives of this work were to confirm PPO resistance in a new Euphorbia heterophylla (EPHHL) biotype, and to compare the electrolyte leakage from R and S to PPO-inhibitors biotypes, using two different methods of incubation in a solution containing herbicides. One experiment was carried in greenhouse and three in laboratory, with a completely randomized design. In the greenhouse experiment, four biotypes of EPHHL were sprayed with seven rates of fomesafen to confirm resistance in suspected biotypes. Leaf disks from R and S EPHHL biotypes in the second and the third experiments and entire leaves in the fourth experiment were incubated in a solution containing PPO-inhibitors to subsequently determine EC of solution. The study confirmed the resistance to PPO-inhibitors in two EPHHL biotypes. There were no significant differences between S and R biotypes in the experiments with the incubation of leaf disks, but incubation of entire leaves of EPHHL S biotype showed higher EC when in a solution with fomesafen, in comparison to the R biotype. The results of this work are an indirect evidence that resistance to PPO-inhibitors is related to lower absorption of herbicide by the shoots and also to some kind of mechanism to cope with oxidative stress.Sociedade Brasileira da Ciência das Plantas Daninhas 2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000300020Planta Daninha v.29 n.3 2011reponame:Planta daninha (Online)instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)instacron:SBCPD10.1590/S0100-83582011000300020info:eu-repo/semantics/openAccessTrezzi,M.MVidal,R.AKruse,N.DGustman,M.SXavier,ERosin,DDedordi,G.Feng2011-10-10T00:00:00Zoai:scielo:S0100-83582011000300020Revistahttp://revistas.cpd.ufv.br/pdaninhaweb/https://old.scielo.br/oai/scielo-oai.php||rpdaninha@gmail.com1806-96810100-8358opendoar:2011-10-10T00:00Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)false |
dc.title.none.fl_str_mv |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
title |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
spellingShingle |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides Trezzi,M.M cell disruption electrical conductivity herbicide absorption resistance mechanism wild poinsettia |
title_short |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
title_full |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
title_fullStr |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
title_full_unstemmed |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
title_sort |
Eletrolite leakage as a technique to diagnose euphorbia heterophylla biotypes resistant to ppo-inhibitors herbicides |
author |
Trezzi,M.M |
author_facet |
Trezzi,M.M Vidal,R.A Kruse,N.D Gustman,M.S Xavier,E Rosin,D Dedordi,G.F |
author_role |
author |
author2 |
Vidal,R.A Kruse,N.D Gustman,M.S Xavier,E Rosin,D Dedordi,G.F |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Trezzi,M.M Vidal,R.A Kruse,N.D Gustman,M.S Xavier,E Rosin,D Dedordi,G.F |
dc.subject.por.fl_str_mv |
cell disruption electrical conductivity herbicide absorption resistance mechanism wild poinsettia |
topic |
cell disruption electrical conductivity herbicide absorption resistance mechanism wild poinsettia |
description |
The action of herbicides that affect the integrity of cell membranes and cause leakage, like PPO-inhibitors, can be detected by measuring the electric conductivity (EC) of a solution in which the plant tissue target is incubated in the presence of herbicide. The objectives of this work were to confirm PPO resistance in a new Euphorbia heterophylla (EPHHL) biotype, and to compare the electrolyte leakage from R and S to PPO-inhibitors biotypes, using two different methods of incubation in a solution containing herbicides. One experiment was carried in greenhouse and three in laboratory, with a completely randomized design. In the greenhouse experiment, four biotypes of EPHHL were sprayed with seven rates of fomesafen to confirm resistance in suspected biotypes. Leaf disks from R and S EPHHL biotypes in the second and the third experiments and entire leaves in the fourth experiment were incubated in a solution containing PPO-inhibitors to subsequently determine EC of solution. The study confirmed the resistance to PPO-inhibitors in two EPHHL biotypes. There were no significant differences between S and R biotypes in the experiments with the incubation of leaf disks, but incubation of entire leaves of EPHHL S biotype showed higher EC when in a solution with fomesafen, in comparison to the R biotype. The results of this work are an indirect evidence that resistance to PPO-inhibitors is related to lower absorption of herbicide by the shoots and also to some kind of mechanism to cope with oxidative stress. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000300020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000300020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-83582011000300020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
dc.source.none.fl_str_mv |
Planta Daninha v.29 n.3 2011 reponame:Planta daninha (Online) instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) instacron:SBCPD |
instname_str |
Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
instacron_str |
SBCPD |
institution |
SBCPD |
reponame_str |
Planta daninha (Online) |
collection |
Planta daninha (Online) |
repository.name.fl_str_mv |
Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
repository.mail.fl_str_mv |
||rpdaninha@gmail.com |
_version_ |
1752126493021962240 |