MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD

Detalhes bibliográficos
Autor(a) principal: SILVA,D.R.O.
Data de Publicação: 2016
Outros Autores: AGOSTINETTO,D., VARGAS,L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Planta daninha (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433
Resumo: ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible generating polymorphisms of high intensity for the separation of biotypes. Cluster analysis revealed five groups, which had a genetic similarity of 53%. The results indicate that all known biotypes are of the same species, but exhibit different varieties. RAPD presents itself as an important tool for characterizing Conyza species.
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spelling MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPDConyza bonariensispolymorphismglyphosatePCRABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible generating polymorphisms of high intensity for the separation of biotypes. Cluster analysis revealed five groups, which had a genetic similarity of 53%. The results indicate that all known biotypes are of the same species, but exhibit different varieties. RAPD presents itself as an important tool for characterizing Conyza species.Sociedade Brasileira da Ciência das Plantas Daninhas 2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433Planta Daninha v.34 n.3 2016reponame:Planta daninha (Online)instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)instacron:SBCPD10.1590/s0100-83582016340300004info:eu-repo/semantics/openAccessSILVA,D.R.O.AGOSTINETTO,D.VARGAS,L.eng2016-11-29T00:00:00Zoai:scielo:S0100-83582016000300433Revistahttp://revistas.cpd.ufv.br/pdaninhaweb/https://old.scielo.br/oai/scielo-oai.php||rpdaninha@gmail.com1806-96810100-8358opendoar:2016-11-29T00:00Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)false
dc.title.none.fl_str_mv MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
title MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
spellingShingle MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
SILVA,D.R.O.
Conyza bonariensis
polymorphism
glyphosate
PCR
title_short MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
title_full MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
title_fullStr MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
title_full_unstemmed MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
title_sort MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD
author SILVA,D.R.O.
author_facet SILVA,D.R.O.
AGOSTINETTO,D.
VARGAS,L.
author_role author
author2 AGOSTINETTO,D.
VARGAS,L.
author2_role author
author
dc.contributor.author.fl_str_mv SILVA,D.R.O.
AGOSTINETTO,D.
VARGAS,L.
dc.subject.por.fl_str_mv Conyza bonariensis
polymorphism
glyphosate
PCR
topic Conyza bonariensis
polymorphism
glyphosate
PCR
description ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in electrophoresis. Biotypes were identified as Conyza bonariensis and microcephala varieties. In molecular analysis, among 25 primers RAPD, eight were reproducible generating polymorphisms of high intensity for the separation of biotypes. Cluster analysis revealed five groups, which had a genetic similarity of 53%. The results indicate that all known biotypes are of the same species, but exhibit different varieties. RAPD presents itself as an important tool for characterizing Conyza species.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300433
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0100-83582016340300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
dc.source.none.fl_str_mv Planta Daninha v.34 n.3 2016
reponame:Planta daninha (Online)
instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)
instacron:SBCPD
instname_str Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)
instacron_str SBCPD
institution SBCPD
reponame_str Planta daninha (Online)
collection Planta daninha (Online)
repository.name.fl_str_mv Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)
repository.mail.fl_str_mv ||rpdaninha@gmail.com
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