Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Planta daninha (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000100109 |
Resumo: | In Brazil, few research works on mechanisms of weed resistance to glyphosate have been conducted so far. Therefore, this research aimed to study analytical procedures determining the relation between the concentration of plant shikimate after glyphosate application and the plant resistance to this herbicide; and evaluate the glyphosate absorption and translocation into two resistant ® and susceptible (S) horseweed biotypes to glyphosate. Horseweed plants with nine true leaves received glyphosate (720 g a.e. ha-1), and 2, 3, 4, 7 and 10 days after application (DAA) the concentration of shikimic acid was measured by HPLC. In another experiment, plants were treated with radiolabeled glyphosate (14C) (1.456 MBq mmol-1 specific activity) and radioactivity was measured 4, 8, 24, 48 and 72 hours after treatment (HAT) by liquid scintillation spectrometry. The shikimate concentration in plants increased 16,351.14 and 7,892.25 mg kg-1 of dry weight, for R and S plants respectively, at seven DAA. Therefore, the procedure for quantification of shikimic acid was suitable for R and S plants differentiation to glyphosate, indicating that the R population is actually resistant to glyphosate. On average, 98% of glyphosate applied was absorbed by the studied biotypes, at 72 HAT. Around 68% of the absorbed radioactivity remained on the biotypes leaves treated, the S biotype showing the highest translocation. Therefore, the R biotype resistance mechanism studied is associated to the differential translocation. |
id |
SBCPD-1_ddb5cf99b1e007ca369f2888e89459cf |
---|---|
oai_identifier_str |
oai:scielo:S0100-83582015000100109 |
network_acronym_str |
SBCPD-1 |
network_name_str |
Planta daninha (Online) |
repository_id_str |
|
spelling |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypesConyza bonariensisHigh Performance Liquid ChromatographyEPSPSselectionradioactivityIn Brazil, few research works on mechanisms of weed resistance to glyphosate have been conducted so far. Therefore, this research aimed to study analytical procedures determining the relation between the concentration of plant shikimate after glyphosate application and the plant resistance to this herbicide; and evaluate the glyphosate absorption and translocation into two resistant ® and susceptible (S) horseweed biotypes to glyphosate. Horseweed plants with nine true leaves received glyphosate (720 g a.e. ha-1), and 2, 3, 4, 7 and 10 days after application (DAA) the concentration of shikimic acid was measured by HPLC. In another experiment, plants were treated with radiolabeled glyphosate (14C) (1.456 MBq mmol-1 specific activity) and radioactivity was measured 4, 8, 24, 48 and 72 hours after treatment (HAT) by liquid scintillation spectrometry. The shikimate concentration in plants increased 16,351.14 and 7,892.25 mg kg-1 of dry weight, for R and S plants respectively, at seven DAA. Therefore, the procedure for quantification of shikimic acid was suitable for R and S plants differentiation to glyphosate, indicating that the R population is actually resistant to glyphosate. On average, 98% of glyphosate applied was absorbed by the studied biotypes, at 72 HAT. Around 68% of the absorbed radioactivity remained on the biotypes leaves treated, the S biotype showing the highest translocation. Therefore, the R biotype resistance mechanism studied is associated to the differential translocation.Sociedade Brasileira da Ciência das Plantas Daninhas 2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000100109Planta Daninha v.33 n.1 2015reponame:Planta daninha (Online)instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)instacron:SBCPD10.1590/S0100-83582015000100013info:eu-repo/semantics/openAccessCARDINALI,V.C.B.DIAS,A.C.R.MUELLER,T.C.ABERCROMBIE,L.STEWART JR.,C.N.TORNISIELO,V.L.CHRISTOFFOLETI,P.J.eng2015-09-01T00:00:00Zoai:scielo:S0100-83582015000100109Revistahttp://revistas.cpd.ufv.br/pdaninhaweb/https://old.scielo.br/oai/scielo-oai.php||rpdaninha@gmail.com1806-96810100-8358opendoar:2015-09-01T00:00Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)false |
dc.title.none.fl_str_mv |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
title |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
spellingShingle |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes CARDINALI,V.C.B. Conyza bonariensis High Performance Liquid Chromatography EPSPS selection radioactivity |
title_short |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
title_full |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
title_fullStr |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
title_full_unstemmed |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
title_sort |
Shikimate accumulation, glyphosate absorption and translocation in horseweed biotypes |
author |
CARDINALI,V.C.B. |
author_facet |
CARDINALI,V.C.B. DIAS,A.C.R. MUELLER,T.C. ABERCROMBIE,L. STEWART JR.,C.N. TORNISIELO,V.L. CHRISTOFFOLETI,P.J. |
author_role |
author |
author2 |
DIAS,A.C.R. MUELLER,T.C. ABERCROMBIE,L. STEWART JR.,C.N. TORNISIELO,V.L. CHRISTOFFOLETI,P.J. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
CARDINALI,V.C.B. DIAS,A.C.R. MUELLER,T.C. ABERCROMBIE,L. STEWART JR.,C.N. TORNISIELO,V.L. CHRISTOFFOLETI,P.J. |
dc.subject.por.fl_str_mv |
Conyza bonariensis High Performance Liquid Chromatography EPSPS selection radioactivity |
topic |
Conyza bonariensis High Performance Liquid Chromatography EPSPS selection radioactivity |
description |
In Brazil, few research works on mechanisms of weed resistance to glyphosate have been conducted so far. Therefore, this research aimed to study analytical procedures determining the relation between the concentration of plant shikimate after glyphosate application and the plant resistance to this herbicide; and evaluate the glyphosate absorption and translocation into two resistant ® and susceptible (S) horseweed biotypes to glyphosate. Horseweed plants with nine true leaves received glyphosate (720 g a.e. ha-1), and 2, 3, 4, 7 and 10 days after application (DAA) the concentration of shikimic acid was measured by HPLC. In another experiment, plants were treated with radiolabeled glyphosate (14C) (1.456 MBq mmol-1 specific activity) and radioactivity was measured 4, 8, 24, 48 and 72 hours after treatment (HAT) by liquid scintillation spectrometry. The shikimate concentration in plants increased 16,351.14 and 7,892.25 mg kg-1 of dry weight, for R and S plants respectively, at seven DAA. Therefore, the procedure for quantification of shikimic acid was suitable for R and S plants differentiation to glyphosate, indicating that the R population is actually resistant to glyphosate. On average, 98% of glyphosate applied was absorbed by the studied biotypes, at 72 HAT. Around 68% of the absorbed radioactivity remained on the biotypes leaves treated, the S biotype showing the highest translocation. Therefore, the R biotype resistance mechanism studied is associated to the differential translocation. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000100109 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000100109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-83582015000100013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
dc.source.none.fl_str_mv |
Planta Daninha v.33 n.1 2015 reponame:Planta daninha (Online) instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) instacron:SBCPD |
instname_str |
Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
instacron_str |
SBCPD |
institution |
SBCPD |
reponame_str |
Planta daninha (Online) |
collection |
Planta daninha (Online) |
repository.name.fl_str_mv |
Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
repository.mail.fl_str_mv |
||rpdaninha@gmail.com |
_version_ |
1752126494445928448 |