Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

• Main Author: Gatiboni,Luciano Colpo
• Publication Date: 2013
• Other Authors: Brunetto,Gustavo, Rheinheimer,Danilo dos Santos, Kaminski,João, Pandolfo,Carla Maria, Veiga,Milton, Flores,Alex Fabiani Claro, Lima,Maria Angélica Silveira, Girotto,Eduardo, Copetti,André Carlos Cruz
• Format: Article
• Language: eng
• Source: Revista Brasileira de Ciência do Solo (Online)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832013000300010
• topic: glycerophosphate; manure; phytate; organic phosphorus; organic fertilization

Long-standing applications of mineral fertilizers or types of organic wastes such as manure can cause phosphorus (P) accumulation and changes in the accumulated P forms in the soil. The objective of this research was to evaluate the forms of P accumulated in soils treated with mineral fertilizer or different types of manure in a long-term experiment. Soil was sampled from the 0-5 cm layer of plots fertilized with five different nutrient sources for nine years: 1) control without fertilizer; 2) mineral fertilizer at recommended rates for local conditions; 3) 5 t ha-1 year-1 of moist poultry litter; 4) 60 m³ ha-1 year-1 of liquid cattle manure and 5) 40 m³ ha-1 year-1 of liquid swine manure. The 31P-NMR spectra of soil extracts detected the following P compounds: orthophosphate, pyrophosphate, inositol phosphate, glycerophosphate, and DNA. The use of organic or mineral fertilizer over nine years did not change the soil P forms but influenced their concentration. Fertilization with mineral or organic fertilizers stimulated P accumulation in inorganic forms. Highest inositol phosphate levels were observed after fertilization with any kind of manure and highest organic P concentration in glycerophosphate form in after mineral or no fertilization.