Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Detalhes bibliográficos
Autor(a) principal: Castro,Jordana Luísa de
Data de Publicação: 2017
Outros Autores: Souza,Mariana Gonçalves, Rufini,Márcia, Guimarães,Amanda Azarias, Rodrigues,Tainara Louzada, Moreira,Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Ciência do Solo (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100421
Resumo: ABSTRACT Mining is an important economic activity. However, its impact on environment must be accessed, mainly on relevant processes for their sustainability. The objective of this study was to evaluate the diversity and efficiency of symbiotic nitrogen fixing bacterial communities in soils under different types of vegetation in the Quadrilátero Ferrífero: ironstone outcrops, Atlantic Forest, neotropical savanna, and a rehabilitated area revegetated with grass. Suspensions of soil samples collected under each type of vegetation were made in a saline solution to capture rhizobia communities that were then inoculated on cowpea [Vigna unguiculata (L.) Walp.], which was used as a trap plant. The symbiotic efficiency of the communities was evaluated in a greenhouse experiment and the data obtained were correlated to the chemical and physical properties of the soils under each type of vegetation. At the end of the experiment, the bacteria present in the nodules were isolated to evaluate their diversity. The highest numbers of nodules occurred in the treatment inoculated with soil samples from rehabilitated area revegetated with grass and neotropical savanna vegetation, and the lowest numbers were observed in the treatment inoculated with soil samples from ironstone outcrops and Atlantic Forest. In relation to root dry matter, the treatment inoculated with soil samples from Neotropical savanah was superior to those inoculated with soil samples from the other areas; already, in relation to the shoot dry matter, no significant difference among the treatments was observed. The soil properties with the greatest influence on the microbial communities were Al3+ content, considered as high in the Atlantic Forest and neotropical savanna vegetation, as intermediate in the iron outcrops, and as very low in the rehabilitated area revegetated with grass; organic matter, considered as very high in the ironstone outcrops and neotropical savanna, as high in the Atlantic Forest, and as low in the rehabilitated area revegetated with grass; and the pH, with intermediate acidity level in the rehabilitated area revegetated with grass, high level of acidity in the iron outcrops and neotropical savanna, and very high acidity in the Atlantic Forest. After isolation of the nodules, 380 bacterial strains were obtained and separated into 27 groups by cultural characterization analysis. Genetic diversity was evaluated by the 16S rRNA gene partial sequencing of 156 strains, which identified some bacteria belonging to nitrogen-fixing Leguminosae nodulating bacterial genera (Rhizobium, Bradyrhizobium, Burkholderia, and Cupriavidus), some representative of associative bacteria (Bacillus, Paenibacillus, Herbaspirillum, Pseudomonas, and Agrobacterium), and other genera (Brevibacillus, Novosphingobium, Chitinophaga, Dyella, Acinetobacter, and Stenotrophomonas). The highest genetic diversity of bacteria was found in the rehabilitated area revegetated with grass indicated that it was effective in soil rehabilitation
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spelling Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plantbiological indicatorsbiological N2 fixationironstone outcropsABSTRACT Mining is an important economic activity. However, its impact on environment must be accessed, mainly on relevant processes for their sustainability. The objective of this study was to evaluate the diversity and efficiency of symbiotic nitrogen fixing bacterial communities in soils under different types of vegetation in the Quadrilátero Ferrífero: ironstone outcrops, Atlantic Forest, neotropical savanna, and a rehabilitated area revegetated with grass. Suspensions of soil samples collected under each type of vegetation were made in a saline solution to capture rhizobia communities that were then inoculated on cowpea [Vigna unguiculata (L.) Walp.], which was used as a trap plant. The symbiotic efficiency of the communities was evaluated in a greenhouse experiment and the data obtained were correlated to the chemical and physical properties of the soils under each type of vegetation. At the end of the experiment, the bacteria present in the nodules were isolated to evaluate their diversity. The highest numbers of nodules occurred in the treatment inoculated with soil samples from rehabilitated area revegetated with grass and neotropical savanna vegetation, and the lowest numbers were observed in the treatment inoculated with soil samples from ironstone outcrops and Atlantic Forest. In relation to root dry matter, the treatment inoculated with soil samples from Neotropical savanah was superior to those inoculated with soil samples from the other areas; already, in relation to the shoot dry matter, no significant difference among the treatments was observed. The soil properties with the greatest influence on the microbial communities were Al3+ content, considered as high in the Atlantic Forest and neotropical savanna vegetation, as intermediate in the iron outcrops, and as very low in the rehabilitated area revegetated with grass; organic matter, considered as very high in the ironstone outcrops and neotropical savanna, as high in the Atlantic Forest, and as low in the rehabilitated area revegetated with grass; and the pH, with intermediate acidity level in the rehabilitated area revegetated with grass, high level of acidity in the iron outcrops and neotropical savanna, and very high acidity in the Atlantic Forest. After isolation of the nodules, 380 bacterial strains were obtained and separated into 27 groups by cultural characterization analysis. Genetic diversity was evaluated by the 16S rRNA gene partial sequencing of 156 strains, which identified some bacteria belonging to nitrogen-fixing Leguminosae nodulating bacterial genera (Rhizobium, Bradyrhizobium, Burkholderia, and Cupriavidus), some representative of associative bacteria (Bacillus, Paenibacillus, Herbaspirillum, Pseudomonas, and Agrobacterium), and other genera (Brevibacillus, Novosphingobium, Chitinophaga, Dyella, Acinetobacter, and Stenotrophomonas). The highest genetic diversity of bacteria was found in the rehabilitated area revegetated with grass indicated that it was effective in soil rehabilitationSociedade Brasileira de Ciência do Solo2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100421Revista Brasileira de Ciência do Solo v.41 2017reponame:Revista Brasileira de Ciência do Solo (Online)instname:Sociedade Brasileira de Ciência do Solo (SBCS)instacron:SBCS10.1590/18069657rbcs20160525info:eu-repo/semantics/openAccessCastro,Jordana Luísa deSouza,Mariana GonçalvesRufini,MárciaGuimarães,Amanda AzariasRodrigues,Tainara LouzadaMoreira,Fatima Maria de Souzaeng2017-08-22T00:00:00Zoai:scielo:S0100-06832017000100421Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=0100-0683&lng=es&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcs@ufv.br1806-96570100-0683opendoar:2017-08-22T00:00Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS)false
dc.title.none.fl_str_mv Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
title Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
spellingShingle Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
Castro,Jordana Luísa de
biological indicators
biological N2 fixation
ironstone outcrops
title_short Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
title_full Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
title_fullStr Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
title_full_unstemmed Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
title_sort Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant
author Castro,Jordana Luísa de
author_facet Castro,Jordana Luísa de
Souza,Mariana Gonçalves
Rufini,Márcia
Guimarães,Amanda Azarias
Rodrigues,Tainara Louzada
Moreira,Fatima Maria de Souza
author_role author
author2 Souza,Mariana Gonçalves
Rufini,Márcia
Guimarães,Amanda Azarias
Rodrigues,Tainara Louzada
Moreira,Fatima Maria de Souza
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Castro,Jordana Luísa de
Souza,Mariana Gonçalves
Rufini,Márcia
Guimarães,Amanda Azarias
Rodrigues,Tainara Louzada
Moreira,Fatima Maria de Souza
dc.subject.por.fl_str_mv biological indicators
biological N2 fixation
ironstone outcrops
topic biological indicators
biological N2 fixation
ironstone outcrops
description ABSTRACT Mining is an important economic activity. However, its impact on environment must be accessed, mainly on relevant processes for their sustainability. The objective of this study was to evaluate the diversity and efficiency of symbiotic nitrogen fixing bacterial communities in soils under different types of vegetation in the Quadrilátero Ferrífero: ironstone outcrops, Atlantic Forest, neotropical savanna, and a rehabilitated area revegetated with grass. Suspensions of soil samples collected under each type of vegetation were made in a saline solution to capture rhizobia communities that were then inoculated on cowpea [Vigna unguiculata (L.) Walp.], which was used as a trap plant. The symbiotic efficiency of the communities was evaluated in a greenhouse experiment and the data obtained were correlated to the chemical and physical properties of the soils under each type of vegetation. At the end of the experiment, the bacteria present in the nodules were isolated to evaluate their diversity. The highest numbers of nodules occurred in the treatment inoculated with soil samples from rehabilitated area revegetated with grass and neotropical savanna vegetation, and the lowest numbers were observed in the treatment inoculated with soil samples from ironstone outcrops and Atlantic Forest. In relation to root dry matter, the treatment inoculated with soil samples from Neotropical savanah was superior to those inoculated with soil samples from the other areas; already, in relation to the shoot dry matter, no significant difference among the treatments was observed. The soil properties with the greatest influence on the microbial communities were Al3+ content, considered as high in the Atlantic Forest and neotropical savanna vegetation, as intermediate in the iron outcrops, and as very low in the rehabilitated area revegetated with grass; organic matter, considered as very high in the ironstone outcrops and neotropical savanna, as high in the Atlantic Forest, and as low in the rehabilitated area revegetated with grass; and the pH, with intermediate acidity level in the rehabilitated area revegetated with grass, high level of acidity in the iron outcrops and neotropical savanna, and very high acidity in the Atlantic Forest. After isolation of the nodules, 380 bacterial strains were obtained and separated into 27 groups by cultural characterization analysis. Genetic diversity was evaluated by the 16S rRNA gene partial sequencing of 156 strains, which identified some bacteria belonging to nitrogen-fixing Leguminosae nodulating bacterial genera (Rhizobium, Bradyrhizobium, Burkholderia, and Cupriavidus), some representative of associative bacteria (Bacillus, Paenibacillus, Herbaspirillum, Pseudomonas, and Agrobacterium), and other genera (Brevibacillus, Novosphingobium, Chitinophaga, Dyella, Acinetobacter, and Stenotrophomonas). The highest genetic diversity of bacteria was found in the rehabilitated area revegetated with grass indicated that it was effective in soil rehabilitation
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100421
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/18069657rbcs20160525
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência do Solo
publisher.none.fl_str_mv Sociedade Brasileira de Ciência do Solo
dc.source.none.fl_str_mv Revista Brasileira de Ciência do Solo v.41 2017
reponame:Revista Brasileira de Ciência do Solo (Online)
instname:Sociedade Brasileira de Ciência do Solo (SBCS)
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