Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique

Detalhes bibliográficos
Autor(a) principal: Heberle,Elaine dos Santos
Data de Publicação: 2015
Outros Autores: Armas,Rafael Dutra de, Heberle,Daniel Alexandre, Stürmer,Sidney Luiz, Peruch,Luiz Augusto Martins, Lovato,Paulo Emílio, Soares,Cláudio Roberto Fonsêca Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Ciência do Solo (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292
Resumo: ABSTRACT Cassava (Manihot esculenta Crantz) is a highly mycotrophic crop, and prior soil cover may affect the density of arbuscular mycorrhizal fungi (AMFs), as well as the composition of the AMFs community in the soil. The aim of this study was to evaluate the occurrence and the structure of AMFs communities in cassava grown after different cover crops, and the effect of the cover crop on mineral nutrition and cassava yield under an organic farming system. The occurrence and structure of the AMFs community was evaluated through polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). A randomized block experimental design was used with four replications. Six different cover crop management systems before cassava were evaluated: black oats, vetch, oilseed radish, intercropped oats + vetch, intercropped oats + vetch + oilseed radish, plus a control (fallow) treatment mowed every 15 days. Oats as a single crop or oats intercropped with vetch or with oilseed radish increased AMFs inoculum potential in soil with a low number of propagules, thus benefiting mycorrhizal colonization of cassava root. The treatments did not affect the structure of AMFs communities in the soil since the AMFs communities were similar in cassava roots in succession to different cover crops. AMFs colonization was high despite high P availability in the soil. The cassava crop yield was above the regional average, and P levels in the leaves were adequate, regardless of which cover crop treatments were used. One cover crop cycle prior to the cassava crop was not enough to observe a significant response in variables, P in plant tissue, crop yield, and occurrence and structure of AMFs communities in the soil. In the cassava roots in succession, the plant developmental stage affected the groupings of the structure of the AMF community.
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spelling Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” TechniqueManihot esculentaarbuscular mycorrhizal fungiroot symbiosesABSTRACT Cassava (Manihot esculenta Crantz) is a highly mycotrophic crop, and prior soil cover may affect the density of arbuscular mycorrhizal fungi (AMFs), as well as the composition of the AMFs community in the soil. The aim of this study was to evaluate the occurrence and the structure of AMFs communities in cassava grown after different cover crops, and the effect of the cover crop on mineral nutrition and cassava yield under an organic farming system. The occurrence and structure of the AMFs community was evaluated through polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). A randomized block experimental design was used with four replications. Six different cover crop management systems before cassava were evaluated: black oats, vetch, oilseed radish, intercropped oats + vetch, intercropped oats + vetch + oilseed radish, plus a control (fallow) treatment mowed every 15 days. Oats as a single crop or oats intercropped with vetch or with oilseed radish increased AMFs inoculum potential in soil with a low number of propagules, thus benefiting mycorrhizal colonization of cassava root. The treatments did not affect the structure of AMFs communities in the soil since the AMFs communities were similar in cassava roots in succession to different cover crops. AMFs colonization was high despite high P availability in the soil. The cassava crop yield was above the regional average, and P levels in the leaves were adequate, regardless of which cover crop treatments were used. One cover crop cycle prior to the cassava crop was not enough to observe a significant response in variables, P in plant tissue, crop yield, and occurrence and structure of AMFs communities in the soil. In the cassava roots in succession, the plant developmental stage affected the groupings of the structure of the AMF community.Sociedade Brasileira de Ciência do Solo2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292Revista Brasileira de Ciência do Solo v.39 n.5 2015reponame:Revista Brasileira de Ciência do Solo (Online)instname:Sociedade Brasileira de Ciência do Solo (SBCS)instacron:SBCS10.1590/01000683rbcs20140216info:eu-repo/semantics/openAccessHeberle,Elaine dos SantosArmas,Rafael Dutra deHeberle,Daniel AlexandreStürmer,Sidney LuizPeruch,Luiz Augusto MartinsLovato,Paulo EmílioSoares,Cláudio Roberto Fonsêca Sousaeng2015-12-04T00:00:00Zoai:scielo:S0100-06832015000501292Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=0100-0683&lng=es&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcs@ufv.br1806-96570100-0683opendoar:2015-12-04T00:00Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS)false
dc.title.none.fl_str_mv Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
title Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
spellingShingle Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
Heberle,Elaine dos Santos
Manihot esculenta
arbuscular mycorrhizal fungi
root symbioses
title_short Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
title_full Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
title_fullStr Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
title_full_unstemmed Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
title_sort Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
author Heberle,Elaine dos Santos
author_facet Heberle,Elaine dos Santos
Armas,Rafael Dutra de
Heberle,Daniel Alexandre
Stürmer,Sidney Luiz
Peruch,Luiz Augusto Martins
Lovato,Paulo Emílio
Soares,Cláudio Roberto Fonsêca Sousa
author_role author
author2 Armas,Rafael Dutra de
Heberle,Daniel Alexandre
Stürmer,Sidney Luiz
Peruch,Luiz Augusto Martins
Lovato,Paulo Emílio
Soares,Cláudio Roberto Fonsêca Sousa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Heberle,Elaine dos Santos
Armas,Rafael Dutra de
Heberle,Daniel Alexandre
Stürmer,Sidney Luiz
Peruch,Luiz Augusto Martins
Lovato,Paulo Emílio
Soares,Cláudio Roberto Fonsêca Sousa
dc.subject.por.fl_str_mv Manihot esculenta
arbuscular mycorrhizal fungi
root symbioses
topic Manihot esculenta
arbuscular mycorrhizal fungi
root symbioses
description ABSTRACT Cassava (Manihot esculenta Crantz) is a highly mycotrophic crop, and prior soil cover may affect the density of arbuscular mycorrhizal fungi (AMFs), as well as the composition of the AMFs community in the soil. The aim of this study was to evaluate the occurrence and the structure of AMFs communities in cassava grown after different cover crops, and the effect of the cover crop on mineral nutrition and cassava yield under an organic farming system. The occurrence and structure of the AMFs community was evaluated through polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). A randomized block experimental design was used with four replications. Six different cover crop management systems before cassava were evaluated: black oats, vetch, oilseed radish, intercropped oats + vetch, intercropped oats + vetch + oilseed radish, plus a control (fallow) treatment mowed every 15 days. Oats as a single crop or oats intercropped with vetch or with oilseed radish increased AMFs inoculum potential in soil with a low number of propagules, thus benefiting mycorrhizal colonization of cassava root. The treatments did not affect the structure of AMFs communities in the soil since the AMFs communities were similar in cassava roots in succession to different cover crops. AMFs colonization was high despite high P availability in the soil. The cassava crop yield was above the regional average, and P levels in the leaves were adequate, regardless of which cover crop treatments were used. One cover crop cycle prior to the cassava crop was not enough to observe a significant response in variables, P in plant tissue, crop yield, and occurrence and structure of AMFs communities in the soil. In the cassava roots in succession, the plant developmental stage affected the groupings of the structure of the AMF community.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/01000683rbcs20140216
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência do Solo
publisher.none.fl_str_mv Sociedade Brasileira de Ciência do Solo
dc.source.none.fl_str_mv Revista Brasileira de Ciência do Solo v.39 n.5 2015
reponame:Revista Brasileira de Ciência do Solo (Online)
instname:Sociedade Brasileira de Ciência do Solo (SBCS)
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repository.name.fl_str_mv Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS)
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