Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Ciência do Solo (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292 |
Resumo: | ABSTRACT Cassava (Manihot esculenta Crantz) is a highly mycotrophic crop, and prior soil cover may affect the density of arbuscular mycorrhizal fungi (AMFs), as well as the composition of the AMFs community in the soil. The aim of this study was to evaluate the occurrence and the structure of AMFs communities in cassava grown after different cover crops, and the effect of the cover crop on mineral nutrition and cassava yield under an organic farming system. The occurrence and structure of the AMFs community was evaluated through polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). A randomized block experimental design was used with four replications. Six different cover crop management systems before cassava were evaluated: black oats, vetch, oilseed radish, intercropped oats + vetch, intercropped oats + vetch + oilseed radish, plus a control (fallow) treatment mowed every 15 days. Oats as a single crop or oats intercropped with vetch or with oilseed radish increased AMFs inoculum potential in soil with a low number of propagules, thus benefiting mycorrhizal colonization of cassava root. The treatments did not affect the structure of AMFs communities in the soil since the AMFs communities were similar in cassava roots in succession to different cover crops. AMFs colonization was high despite high P availability in the soil. The cassava crop yield was above the regional average, and P levels in the leaves were adequate, regardless of which cover crop treatments were used. One cover crop cycle prior to the cassava crop was not enough to observe a significant response in variables, P in plant tissue, crop yield, and occurrence and structure of AMFs communities in the soil. In the cassava roots in succession, the plant developmental stage affected the groupings of the structure of the AMF community. |
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Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” TechniqueManihot esculentaarbuscular mycorrhizal fungiroot symbiosesABSTRACT Cassava (Manihot esculenta Crantz) is a highly mycotrophic crop, and prior soil cover may affect the density of arbuscular mycorrhizal fungi (AMFs), as well as the composition of the AMFs community in the soil. The aim of this study was to evaluate the occurrence and the structure of AMFs communities in cassava grown after different cover crops, and the effect of the cover crop on mineral nutrition and cassava yield under an organic farming system. The occurrence and structure of the AMFs community was evaluated through polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). A randomized block experimental design was used with four replications. Six different cover crop management systems before cassava were evaluated: black oats, vetch, oilseed radish, intercropped oats + vetch, intercropped oats + vetch + oilseed radish, plus a control (fallow) treatment mowed every 15 days. Oats as a single crop or oats intercropped with vetch or with oilseed radish increased AMFs inoculum potential in soil with a low number of propagules, thus benefiting mycorrhizal colonization of cassava root. The treatments did not affect the structure of AMFs communities in the soil since the AMFs communities were similar in cassava roots in succession to different cover crops. AMFs colonization was high despite high P availability in the soil. The cassava crop yield was above the regional average, and P levels in the leaves were adequate, regardless of which cover crop treatments were used. One cover crop cycle prior to the cassava crop was not enough to observe a significant response in variables, P in plant tissue, crop yield, and occurrence and structure of AMFs communities in the soil. In the cassava roots in succession, the plant developmental stage affected the groupings of the structure of the AMF community.Sociedade Brasileira de Ciência do Solo2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292Revista Brasileira de Ciência do Solo v.39 n.5 2015reponame:Revista Brasileira de Ciência do Solo (Online)instname:Sociedade Brasileira de Ciência do Solo (SBCS)instacron:SBCS10.1590/01000683rbcs20140216info:eu-repo/semantics/openAccessHeberle,Elaine dos SantosArmas,Rafael Dutra deHeberle,Daniel AlexandreStürmer,Sidney LuizPeruch,Luiz Augusto MartinsLovato,Paulo EmílioSoares,Cláudio Roberto Fonsêca Sousaeng2015-12-04T00:00:00Zoai:scielo:S0100-06832015000501292Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=0100-0683&lng=es&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcs@ufv.br1806-96570100-0683opendoar:2015-12-04T00:00Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS)false |
dc.title.none.fl_str_mv |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
title |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
spellingShingle |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique Heberle,Elaine dos Santos Manihot esculenta arbuscular mycorrhizal fungi root symbioses |
title_short |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
title_full |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
title_fullStr |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
title_full_unstemmed |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
title_sort |
Occurrence and Structure of Arbuscular Mycorrhizal Fungal Communities in Cassava after Cultivation of Cover Crops as Observed by the “PCR-DGGE” Technique |
author |
Heberle,Elaine dos Santos |
author_facet |
Heberle,Elaine dos Santos Armas,Rafael Dutra de Heberle,Daniel Alexandre Stürmer,Sidney Luiz Peruch,Luiz Augusto Martins Lovato,Paulo Emílio Soares,Cláudio Roberto Fonsêca Sousa |
author_role |
author |
author2 |
Armas,Rafael Dutra de Heberle,Daniel Alexandre Stürmer,Sidney Luiz Peruch,Luiz Augusto Martins Lovato,Paulo Emílio Soares,Cláudio Roberto Fonsêca Sousa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Heberle,Elaine dos Santos Armas,Rafael Dutra de Heberle,Daniel Alexandre Stürmer,Sidney Luiz Peruch,Luiz Augusto Martins Lovato,Paulo Emílio Soares,Cláudio Roberto Fonsêca Sousa |
dc.subject.por.fl_str_mv |
Manihot esculenta arbuscular mycorrhizal fungi root symbioses |
topic |
Manihot esculenta arbuscular mycorrhizal fungi root symbioses |
description |
ABSTRACT Cassava (Manihot esculenta Crantz) is a highly mycotrophic crop, and prior soil cover may affect the density of arbuscular mycorrhizal fungi (AMFs), as well as the composition of the AMFs community in the soil. The aim of this study was to evaluate the occurrence and the structure of AMFs communities in cassava grown after different cover crops, and the effect of the cover crop on mineral nutrition and cassava yield under an organic farming system. The occurrence and structure of the AMFs community was evaluated through polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). A randomized block experimental design was used with four replications. Six different cover crop management systems before cassava were evaluated: black oats, vetch, oilseed radish, intercropped oats + vetch, intercropped oats + vetch + oilseed radish, plus a control (fallow) treatment mowed every 15 days. Oats as a single crop or oats intercropped with vetch or with oilseed radish increased AMFs inoculum potential in soil with a low number of propagules, thus benefiting mycorrhizal colonization of cassava root. The treatments did not affect the structure of AMFs communities in the soil since the AMFs communities were similar in cassava roots in succession to different cover crops. AMFs colonization was high despite high P availability in the soil. The cassava crop yield was above the regional average, and P levels in the leaves were adequate, regardless of which cover crop treatments were used. One cover crop cycle prior to the cassava crop was not enough to observe a significant response in variables, P in plant tissue, crop yield, and occurrence and structure of AMFs communities in the soil. In the cassava roots in succession, the plant developmental stage affected the groupings of the structure of the AMF community. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000501292 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/01000683rbcs20140216 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência do Solo |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência do Solo |
dc.source.none.fl_str_mv |
Revista Brasileira de Ciência do Solo v.39 n.5 2015 reponame:Revista Brasileira de Ciência do Solo (Online) instname:Sociedade Brasileira de Ciência do Solo (SBCS) instacron:SBCS |
instname_str |
Sociedade Brasileira de Ciência do Solo (SBCS) |
instacron_str |
SBCS |
institution |
SBCS |
reponame_str |
Revista Brasileira de Ciência do Solo (Online) |
collection |
Revista Brasileira de Ciência do Solo (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS) |
repository.mail.fl_str_mv |
||sbcs@ufv.br |
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1752126520418107392 |