Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability

Detalhes bibliográficos
Autor(a) principal: KIM,Seo-Hee
Data de Publicação: 2022
Outros Autores: YIM,Soon-Ho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101260
Resumo: Abstract Cytotoxic activity profiling of the n-hexane extract from the fruit pulp of Ginkgo biloba L. (Gingkgoaceae) led to the purification of 8 compounds. HPLC based extract profiling identified a cytotoxic alkenylresorcinol. The extract was further separated by time-based fractionation in a gradient HPLC condition, and cytotoxicity of each fraction was evaluated using 293, B16F10, BJAB, and HCT116 cancer cells in vitro. Bilobol was isolated as an active constituent by semi-preparative HPLC and showed significant cytotoxic activities against the cancer cells in a dose-dependent manner at the concentrations of 15.0 ~ 50 μg/mL. The western blotting analysis showed that this compound increased expression of active caspase-3 and active caspase-8 in HCT116 human colon cancer cells, indicating that this compound causes apoptotic cell death in a dose-dependent manner. The results suggest that bilobol is the active component from fruit pulp of Ginkgo Biloba L. that can induce apoptotic cell death in various cancer cells.
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spelling Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viabilitybilobolcell viabilityHPLCfruit pulpcaspase-3,8Abstract Cytotoxic activity profiling of the n-hexane extract from the fruit pulp of Ginkgo biloba L. (Gingkgoaceae) led to the purification of 8 compounds. HPLC based extract profiling identified a cytotoxic alkenylresorcinol. The extract was further separated by time-based fractionation in a gradient HPLC condition, and cytotoxicity of each fraction was evaluated using 293, B16F10, BJAB, and HCT116 cancer cells in vitro. Bilobol was isolated as an active constituent by semi-preparative HPLC and showed significant cytotoxic activities against the cancer cells in a dose-dependent manner at the concentrations of 15.0 ~ 50 μg/mL. The western blotting analysis showed that this compound increased expression of active caspase-3 and active caspase-8 in HCT116 human colon cancer cells, indicating that this compound causes apoptotic cell death in a dose-dependent manner. The results suggest that bilobol is the active component from fruit pulp of Ginkgo Biloba L. that can induce apoptotic cell death in various cancer cells.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101260Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.57522info:eu-repo/semantics/openAccessKIM,Seo-HeeYIM,Soon-Hoeng2022-07-26T00:00:00Zoai:scielo:S0101-20612022000101260Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-07-26T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
title Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
spellingShingle Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
KIM,Seo-Hee
bilobol
cell viability
HPLC
fruit pulp
caspase-3,8
title_short Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
title_full Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
title_fullStr Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
title_full_unstemmed Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
title_sort Effects of Bilobol from the Fruit Pulp of Ginkgo biloba on Cell Viability
author KIM,Seo-Hee
author_facet KIM,Seo-Hee
YIM,Soon-Ho
author_role author
author2 YIM,Soon-Ho
author2_role author
dc.contributor.author.fl_str_mv KIM,Seo-Hee
YIM,Soon-Ho
dc.subject.por.fl_str_mv bilobol
cell viability
HPLC
fruit pulp
caspase-3,8
topic bilobol
cell viability
HPLC
fruit pulp
caspase-3,8
description Abstract Cytotoxic activity profiling of the n-hexane extract from the fruit pulp of Ginkgo biloba L. (Gingkgoaceae) led to the purification of 8 compounds. HPLC based extract profiling identified a cytotoxic alkenylresorcinol. The extract was further separated by time-based fractionation in a gradient HPLC condition, and cytotoxicity of each fraction was evaluated using 293, B16F10, BJAB, and HCT116 cancer cells in vitro. Bilobol was isolated as an active constituent by semi-preparative HPLC and showed significant cytotoxic activities against the cancer cells in a dose-dependent manner at the concentrations of 15.0 ~ 50 μg/mL. The western blotting analysis showed that this compound increased expression of active caspase-3 and active caspase-8 in HCT116 human colon cancer cells, indicating that this compound causes apoptotic cell death in a dose-dependent manner. The results suggest that bilobol is the active component from fruit pulp of Ginkgo Biloba L. that can induce apoptotic cell death in various cancer cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101260
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101260
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.57522
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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