Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101176 |
Resumo: | Abstract The food industry shows great interest on researching innovative and alternative methods to determine phenolics compounds’. Hence, the present study aims to develop a technique based on Folin-Ciocalteu’s method to determine the total amount of phenolic compounds of a sample using a smartphone as a tool for obtaining digital images of the analysis’ and evaluating them through the Colorgrab® application. In this study, color parameters S, L, V and I were evaluated, as well as experimental parameters such as distance between sample and camera, light temperature, and sample’s volume. To improve analytical conditions, phenolic compounds were determined through the conventional Folin-Ciocalteu’s method, with the results being compared to the data obtained from the adapted method. After optimization, the procedure was carried out using the S color parameter, cold light (6500 K), 7 cm distance between sample and camera, and a solution volume of 1.5 mL. The test showed suitable results for precision (RSD < 2.4%) and accuracy (98.4 to 103.8%) when compared to the conventional method. The proposed technique proved to be an innovative alternative to determine total phenolic compounds in routine analysis for different sectors of the food industry, showing good analytical results without requiring sophisticated equipment. |
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Food Science and Technology (Campinas) |
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Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital imagesPractical Application: This study sought to develop an analytical method through the use of digital images obtained from a smartphone as an easily available alternative to quantify a sample’s total phenolic compounds. This technique aims to replace the use of a spectrophotometerreducing the need for bulky equipmentas well as reducing environmental impacts by using less solventsall while proposing a technique with relatively lower cost compared to those commonly used and following the recommendations of green chemistryAbstract The food industry shows great interest on researching innovative and alternative methods to determine phenolics compounds’. Hence, the present study aims to develop a technique based on Folin-Ciocalteu’s method to determine the total amount of phenolic compounds of a sample using a smartphone as a tool for obtaining digital images of the analysis’ and evaluating them through the Colorgrab® application. In this study, color parameters S, L, V and I were evaluated, as well as experimental parameters such as distance between sample and camera, light temperature, and sample’s volume. To improve analytical conditions, phenolic compounds were determined through the conventional Folin-Ciocalteu’s method, with the results being compared to the data obtained from the adapted method. After optimization, the procedure was carried out using the S color parameter, cold light (6500 K), 7 cm distance between sample and camera, and a solution volume of 1.5 mL. The test showed suitable results for precision (RSD < 2.4%) and accuracy (98.4 to 103.8%) when compared to the conventional method. The proposed technique proved to be an innovative alternative to determine total phenolic compounds in routine analysis for different sectors of the food industry, showing good analytical results without requiring sophisticated equipment.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101176Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.35122info:eu-repo/semantics/openAccessLUCAS,Bruna NichelleDALLA NORA,BOEIRA,Caroline PagnossimVERRUCK,SilvaniROSA,Claudia Severo daeng2022-06-01T00:00:00Zoai:scielo:S0101-20612022000101176Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-06-01T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
title |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
spellingShingle |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images LUCAS,Bruna Nichelle Practical Application: This study sought to develop an analytical method through the use of digital images obtained from a smartphone as an easily available alternative to quantify a sample’s total phenolic compounds. This technique aims to replace the use of a spectrophotometer reducing the need for bulky equipment as well as reducing environmental impacts by using less solvents all while proposing a technique with relatively lower cost compared to those commonly used and following the recommendations of green chemistry |
title_short |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
title_full |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
title_fullStr |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
title_full_unstemmed |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
title_sort |
Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images |
author |
LUCAS,Bruna Nichelle |
author_facet |
LUCAS,Bruna Nichelle DALLA NORA, BOEIRA,Caroline Pagnossim VERRUCK,Silvani ROSA,Claudia Severo da |
author_role |
author |
author2 |
DALLA NORA, BOEIRA,Caroline Pagnossim VERRUCK,Silvani ROSA,Claudia Severo da |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
LUCAS,Bruna Nichelle DALLA NORA, BOEIRA,Caroline Pagnossim VERRUCK,Silvani ROSA,Claudia Severo da |
dc.subject.por.fl_str_mv |
Practical Application: This study sought to develop an analytical method through the use of digital images obtained from a smartphone as an easily available alternative to quantify a sample’s total phenolic compounds. This technique aims to replace the use of a spectrophotometer reducing the need for bulky equipment as well as reducing environmental impacts by using less solvents all while proposing a technique with relatively lower cost compared to those commonly used and following the recommendations of green chemistry |
topic |
Practical Application: This study sought to develop an analytical method through the use of digital images obtained from a smartphone as an easily available alternative to quantify a sample’s total phenolic compounds. This technique aims to replace the use of a spectrophotometer reducing the need for bulky equipment as well as reducing environmental impacts by using less solvents all while proposing a technique with relatively lower cost compared to those commonly used and following the recommendations of green chemistry |
description |
Abstract The food industry shows great interest on researching innovative and alternative methods to determine phenolics compounds’. Hence, the present study aims to develop a technique based on Folin-Ciocalteu’s method to determine the total amount of phenolic compounds of a sample using a smartphone as a tool for obtaining digital images of the analysis’ and evaluating them through the Colorgrab® application. In this study, color parameters S, L, V and I were evaluated, as well as experimental parameters such as distance between sample and camera, light temperature, and sample’s volume. To improve analytical conditions, phenolic compounds were determined through the conventional Folin-Ciocalteu’s method, with the results being compared to the data obtained from the adapted method. After optimization, the procedure was carried out using the S color parameter, cold light (6500 K), 7 cm distance between sample and camera, and a solution volume of 1.5 mL. The test showed suitable results for precision (RSD < 2.4%) and accuracy (98.4 to 103.8%) when compared to the conventional method. The proposed technique proved to be an innovative alternative to determine total phenolic compounds in routine analysis for different sectors of the food industry, showing good analytical results without requiring sophisticated equipment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101176 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.35122 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126334629314560 |