Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600475 |
Resumo: | Abstract This study aimed to evaluate whether dihydroferulic acid (dFA) promoted the viability of H2O2-treated PC12 cells and functional recovery from ischemic injury. The animals were divided into four groups for the study: (1) the vehicle treated (saline, 1 mL/kg), (2) dFA 5 mg/kg treated, (3) dFA10 mg/kg treated, and (4) dFA 20 mg/kg treated groups. Neurological deficit was evaluated using the modified neurological severity score. Real-time polymerase chain reaction analyses were performed with the protein disulphide isomerase (PDI), nuclear factor-E2-related factor 2 (Nrf2), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) genes. Immunohistochemical analysis was performed with the Iba-1 and MFG-E8 genes. dFA treatment improved the reduced viability of PC12 cells induced by H2O2 in a dose-dependent manner. Only 50 μM of dFA significantly enhanced the transcription levels of antioxidant genes and neurotrophic factors compared to the vehicle group. In vivo dFA administration exerted a neuroprotective effect by reducing the infarct volume and enhancing behavioral function following cerebral ischemia. dFA treatment protected neuronal cells from ischemic injury and increased the transcription levels of anti-oxidant genes (PDI and Nrf2) and neurotrophic factors (BDNF and NGF). dFA treatment decreased the expression of Iba-1 and MFG-E8 genes, which signal neural cell death. |
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Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat modelfermented rice bran extractdihydroferulic acidanti-oxidant genesischemic brain injuryneuroprotective effectAbstract This study aimed to evaluate whether dihydroferulic acid (dFA) promoted the viability of H2O2-treated PC12 cells and functional recovery from ischemic injury. The animals were divided into four groups for the study: (1) the vehicle treated (saline, 1 mL/kg), (2) dFA 5 mg/kg treated, (3) dFA10 mg/kg treated, and (4) dFA 20 mg/kg treated groups. Neurological deficit was evaluated using the modified neurological severity score. Real-time polymerase chain reaction analyses were performed with the protein disulphide isomerase (PDI), nuclear factor-E2-related factor 2 (Nrf2), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) genes. Immunohistochemical analysis was performed with the Iba-1 and MFG-E8 genes. dFA treatment improved the reduced viability of PC12 cells induced by H2O2 in a dose-dependent manner. Only 50 μM of dFA significantly enhanced the transcription levels of antioxidant genes and neurotrophic factors compared to the vehicle group. In vivo dFA administration exerted a neuroprotective effect by reducing the infarct volume and enhancing behavioral function following cerebral ischemia. dFA treatment protected neuronal cells from ischemic injury and increased the transcription levels of anti-oxidant genes (PDI and Nrf2) and neurotrophic factors (BDNF and NGF). dFA treatment decreased the expression of Iba-1 and MFG-E8 genes, which signal neural cell death.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2020-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600475Food Science and Technology v.40 suppl.2 2020reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.33719info:eu-repo/semantics/openAccessLEE,Sang HyunYEO,DanaHONG,Jeong Hwaeng2020-11-23T00:00:00Zoai:scielo:S0101-20612020000600475Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2020-11-23T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
title |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
spellingShingle |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model LEE,Sang Hyun fermented rice bran extract dihydroferulic acid anti-oxidant genes ischemic brain injury neuroprotective effect |
title_short |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
title_full |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
title_fullStr |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
title_full_unstemmed |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
title_sort |
Effect of dihydroferulic acid obtained from fermented rice bran extract on neuroprotection and behavioral recovery in an ischemic rat model |
author |
LEE,Sang Hyun |
author_facet |
LEE,Sang Hyun YEO,Dana HONG,Jeong Hwa |
author_role |
author |
author2 |
YEO,Dana HONG,Jeong Hwa |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
LEE,Sang Hyun YEO,Dana HONG,Jeong Hwa |
dc.subject.por.fl_str_mv |
fermented rice bran extract dihydroferulic acid anti-oxidant genes ischemic brain injury neuroprotective effect |
topic |
fermented rice bran extract dihydroferulic acid anti-oxidant genes ischemic brain injury neuroprotective effect |
description |
Abstract This study aimed to evaluate whether dihydroferulic acid (dFA) promoted the viability of H2O2-treated PC12 cells and functional recovery from ischemic injury. The animals were divided into four groups for the study: (1) the vehicle treated (saline, 1 mL/kg), (2) dFA 5 mg/kg treated, (3) dFA10 mg/kg treated, and (4) dFA 20 mg/kg treated groups. Neurological deficit was evaluated using the modified neurological severity score. Real-time polymerase chain reaction analyses were performed with the protein disulphide isomerase (PDI), nuclear factor-E2-related factor 2 (Nrf2), nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) genes. Immunohistochemical analysis was performed with the Iba-1 and MFG-E8 genes. dFA treatment improved the reduced viability of PC12 cells induced by H2O2 in a dose-dependent manner. Only 50 μM of dFA significantly enhanced the transcription levels of antioxidant genes and neurotrophic factors compared to the vehicle group. In vivo dFA administration exerted a neuroprotective effect by reducing the infarct volume and enhancing behavioral function following cerebral ischemia. dFA treatment protected neuronal cells from ischemic injury and increased the transcription levels of anti-oxidant genes (PDI and Nrf2) and neurotrophic factors (BDNF and NGF). dFA treatment decreased the expression of Iba-1 and MFG-E8 genes, which signal neural cell death. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600475 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600475 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.33719 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.40 suppl.2 2020 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126326854123520 |