Extraction of ginsenoside Rg2 from stems-leaves of Panax ginseng and its protective effect on myocardial injury in rats with sepsis

• Autor(a) principal: XU,Yuansheng
• Data de Publicação: 2022
• Outros Autores: WANG,Yi, FANG,Jinyan, DONG,Shuangyong
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Food Science and Technology (Campinas)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101169
• topic: ginsenoside Rg2; myocardial injury; sepsis; SIRT1; NF-κB

Abstract This study prepared ginsenoside Rg2 (GRg2) and investigated its protective effect on myocardial injury in sepsis rats. Fifty rats were divided into sham, lipopolysaccharide (LPS), LPS+GRg2, LPS+GRg2+EX527 and LPS+EX527 groups. The latter three groups were treated with 20 mg/kg GRg2, 20 mg/kg GRg2 plus 5 mg/kg EX527 and 5 mg/kg EX527, respectively. Then, the LPS-induced sepsis model was made in the latter four groups. Results showed that, after 7 h from modeling, compared with LPS group, in LPS+GRg2 group, the left ventricular end-diastolic diameter and left ventricular end-systolic diameter were lowered, the left ventricular fraction shortening and left ventricular ejection fraction were elevated, the serum creatine kinase isoenzyme MB, cardiac troponin I, tumor necrosis factor α and interleukin 6 levels were decreased, the myocardial superoxide dismutase level was increased, the myocardial malondialdehyde level was significantly decreased, the myocardial B-cell lymphoma-2 expression level was increased, and the myocardial B-cell lymphoma-2 associated X level was obviously decreased, the myocardial silent information regulator 1 (SIRT1) level was increased, and the myocardial nuclear factor kappa-B (NF-κB) p65 level was decreased. In conclusion, GRg2 can alleviate myocardial injury in sepsis rats through reducing inflammatory response and oxidative stress and regulating apoptosis and SIRT1/NF-κB signaling pathways.