Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100691 |
Resumo: | Abstract Cryptotanshinone (CPT), a terpenoid isolated from the roots of Salvia miltiorrhiza Bunge, was reported to have neuroprotective effects against cerebral ischemic stroke. However, the exact molecular mechanism underlying its neuroprotective ability remains unclear. The present study aimed to explore the regulatory effects of CPT on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced cell injury in a model of hippocampal neurons. Our results demonstrated that CPT improved cell viability and reduced the lactate dehydrogenase leakage in OGD/R-stimulated hippocampal neurons. In addition, CPT significantly inhibited oxidative stress and apoptosis in hippocampal neurons after OGD/R stimulation. Furthermore, CPT significantly enhanced the nuclear translocation of the nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression in hippocampal neurons exposed to OGD/R. Moreover, Nrf2 knockdown reversed the anti-apoptotic and anti-oxidant activities of CPT in primary hippocampal neurons exposed to OGD/R. In conclusion, these findings demonstrated that CPT attenuated oxidative stress and neuronal apoptosis after OGD/R injury through the activation of Nrf2/HO-1 signaling pathway in hippocampal neurons. Thus, CPT may be a novel therapeutic agent for cerebral I/R injury. |
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Food Science and Technology (Campinas) |
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Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathwaycerebral ischemia/reperfusion (I/R) injuryCryptotanshinonehippocampal neuronsneuroprotectiveNrf2/HO-1 pathwayoxidative stressAbstract Cryptotanshinone (CPT), a terpenoid isolated from the roots of Salvia miltiorrhiza Bunge, was reported to have neuroprotective effects against cerebral ischemic stroke. However, the exact molecular mechanism underlying its neuroprotective ability remains unclear. The present study aimed to explore the regulatory effects of CPT on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced cell injury in a model of hippocampal neurons. Our results demonstrated that CPT improved cell viability and reduced the lactate dehydrogenase leakage in OGD/R-stimulated hippocampal neurons. In addition, CPT significantly inhibited oxidative stress and apoptosis in hippocampal neurons after OGD/R stimulation. Furthermore, CPT significantly enhanced the nuclear translocation of the nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression in hippocampal neurons exposed to OGD/R. Moreover, Nrf2 knockdown reversed the anti-apoptotic and anti-oxidant activities of CPT in primary hippocampal neurons exposed to OGD/R. In conclusion, these findings demonstrated that CPT attenuated oxidative stress and neuronal apoptosis after OGD/R injury through the activation of Nrf2/HO-1 signaling pathway in hippocampal neurons. Thus, CPT may be a novel therapeutic agent for cerebral I/R injury.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100691Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.46521info:eu-repo/semantics/openAccessXU,DongGUI,ChengliZHAO,HaiyanLIU,Fenglieng2022-02-23T00:00:00Zoai:scielo:S0101-20612022000100691Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-02-23T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
title |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
spellingShingle |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway XU,Dong cerebral ischemia/reperfusion (I/R) injury Cryptotanshinone hippocampal neurons neuroprotective Nrf2/HO-1 pathway oxidative stress |
title_short |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
title_full |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
title_fullStr |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
title_full_unstemmed |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
title_sort |
Cryptotanshinone protects hippocampal neurons against oxygen-glucose deprivation-induced injury through the activation of Nrf2/HO-1 signaling pathway |
author |
XU,Dong |
author_facet |
XU,Dong GUI,Chengli ZHAO,Haiyan LIU,Fengli |
author_role |
author |
author2 |
GUI,Chengli ZHAO,Haiyan LIU,Fengli |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
XU,Dong GUI,Chengli ZHAO,Haiyan LIU,Fengli |
dc.subject.por.fl_str_mv |
cerebral ischemia/reperfusion (I/R) injury Cryptotanshinone hippocampal neurons neuroprotective Nrf2/HO-1 pathway oxidative stress |
topic |
cerebral ischemia/reperfusion (I/R) injury Cryptotanshinone hippocampal neurons neuroprotective Nrf2/HO-1 pathway oxidative stress |
description |
Abstract Cryptotanshinone (CPT), a terpenoid isolated from the roots of Salvia miltiorrhiza Bunge, was reported to have neuroprotective effects against cerebral ischemic stroke. However, the exact molecular mechanism underlying its neuroprotective ability remains unclear. The present study aimed to explore the regulatory effects of CPT on oxygen-glucose deprivation/reoxygenation (OGD/R)-induced cell injury in a model of hippocampal neurons. Our results demonstrated that CPT improved cell viability and reduced the lactate dehydrogenase leakage in OGD/R-stimulated hippocampal neurons. In addition, CPT significantly inhibited oxidative stress and apoptosis in hippocampal neurons after OGD/R stimulation. Furthermore, CPT significantly enhanced the nuclear translocation of the nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression in hippocampal neurons exposed to OGD/R. Moreover, Nrf2 knockdown reversed the anti-apoptotic and anti-oxidant activities of CPT in primary hippocampal neurons exposed to OGD/R. In conclusion, these findings demonstrated that CPT attenuated oxidative stress and neuronal apoptosis after OGD/R injury through the activation of Nrf2/HO-1 signaling pathway in hippocampal neurons. Thus, CPT may be a novel therapeutic agent for cerebral I/R injury. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100691 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100691 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.46521 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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1752126332481830912 |