In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600634 |
Resumo: | Abstract New insights on the use of peptides as therapeutic agents in the treatment of cancer have emerged with reports showing anti-tumour activity of peptides, predominantly derived from animals or microorganisms. Amaranth is a pseudocereal traditionally acknowledged to possess pharmacotherapeutic properties. Thus, the aim of this study was to compare the in vitro anti-cancer effect of amaranth protein hydrolysates (alcalase, trypsin, and pepsin). Protein hydrolysates were tested for their antioxidant activity together with the anticancer and apoptotic potential. Antioxidants results revealed hydrolysates to have a greater antioxidant effect than un-hydrolysed protein, with results exceeding that of controls. The MTT cytotoxicity assay conducted on MCF-7, A549 and HEK 293 cell lines showed the trypsin hydrolysate to exhibit a preeminent anti-cancer effect. Annexin V-FITC flow cytometry showed an increased number of early apoptotic and late necrotic cells compared to untreated cells, further validated by caspase 3/7 activity. These assays confirmed the induction of apoptosis of the trypsin hydrolysate thereby demonstrating that the hydrolysate can be used as a potential therapeutic against selected cancers. |
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Food Science and Technology (Campinas) |
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In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysatesAmaranthus cruentusantioxidantcytotoxicityapoptosisAbstract New insights on the use of peptides as therapeutic agents in the treatment of cancer have emerged with reports showing anti-tumour activity of peptides, predominantly derived from animals or microorganisms. Amaranth is a pseudocereal traditionally acknowledged to possess pharmacotherapeutic properties. Thus, the aim of this study was to compare the in vitro anti-cancer effect of amaranth protein hydrolysates (alcalase, trypsin, and pepsin). Protein hydrolysates were tested for their antioxidant activity together with the anticancer and apoptotic potential. Antioxidants results revealed hydrolysates to have a greater antioxidant effect than un-hydrolysed protein, with results exceeding that of controls. The MTT cytotoxicity assay conducted on MCF-7, A549 and HEK 293 cell lines showed the trypsin hydrolysate to exhibit a preeminent anti-cancer effect. Annexin V-FITC flow cytometry showed an increased number of early apoptotic and late necrotic cells compared to untreated cells, further validated by caspase 3/7 activity. These assays confirmed the induction of apoptosis of the trypsin hydrolysate thereby demonstrating that the hydrolysate can be used as a potential therapeutic against selected cancers.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2020-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600634Food Science and Technology v.40 suppl.2 2020reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.36219info:eu-repo/semantics/openAccessRAMKISSON,ShaneceDWARKA,DepikaVENTER,SonjaMELLEM,John Jasoneng2020-11-23T00:00:00Zoai:scielo:S0101-20612020000600634Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2020-11-23T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
title |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
spellingShingle |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates RAMKISSON,Shanece Amaranthus cruentus antioxidant cytotoxicity apoptosis |
title_short |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
title_full |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
title_fullStr |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
title_full_unstemmed |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
title_sort |
In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates |
author |
RAMKISSON,Shanece |
author_facet |
RAMKISSON,Shanece DWARKA,Depika VENTER,Sonja MELLEM,John Jason |
author_role |
author |
author2 |
DWARKA,Depika VENTER,Sonja MELLEM,John Jason |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
RAMKISSON,Shanece DWARKA,Depika VENTER,Sonja MELLEM,John Jason |
dc.subject.por.fl_str_mv |
Amaranthus cruentus antioxidant cytotoxicity apoptosis |
topic |
Amaranthus cruentus antioxidant cytotoxicity apoptosis |
description |
Abstract New insights on the use of peptides as therapeutic agents in the treatment of cancer have emerged with reports showing anti-tumour activity of peptides, predominantly derived from animals or microorganisms. Amaranth is a pseudocereal traditionally acknowledged to possess pharmacotherapeutic properties. Thus, the aim of this study was to compare the in vitro anti-cancer effect of amaranth protein hydrolysates (alcalase, trypsin, and pepsin). Protein hydrolysates were tested for their antioxidant activity together with the anticancer and apoptotic potential. Antioxidants results revealed hydrolysates to have a greater antioxidant effect than un-hydrolysed protein, with results exceeding that of controls. The MTT cytotoxicity assay conducted on MCF-7, A549 and HEK 293 cell lines showed the trypsin hydrolysate to exhibit a preeminent anti-cancer effect. Annexin V-FITC flow cytometry showed an increased number of early apoptotic and late necrotic cells compared to untreated cells, further validated by caspase 3/7 activity. These assays confirmed the induction of apoptosis of the trypsin hydrolysate thereby demonstrating that the hydrolysate can be used as a potential therapeutic against selected cancers. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600634 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000600634 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.36219 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.40 suppl.2 2020 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126326867755008 |