A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts

Detalhes bibliográficos
Autor(a) principal: XU,Ying-hao
Data de Publicação: 2022
Outros Autores: FU,Xin, LU,Ming, WEI,Bao-dong
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101099
Resumo: Abstract Pea sprouts are derived from sprouted peas and are popular for their high vitamin C and flavonoid content, as well as their rapid growth and ease of production. Enhancing its nutritional value with simple treatments is worth exploring, In the present study, we applied supplemental UV irradiation to pea sprouts and examined various components such as vitamin C and flavonoid substances in the treated pea sprouts. Meanwhile, to investigate the mechanism of action of UV irradiation on pea sprouts, we performed high-throughput RNA sequencing (RNA-Seq) analysis to probe the genome-wide expression profile of pea sprouts under UV irradiation, generating 41.90 Gb of Clean Data, the results were also validated by reverse transcription-polymerase chain reaction (RT-qPCR), and the raw data of sequencing were submitted to the SRA repository. Further transcriptome analysis showed that UV irradiation significantly affected the expression of genes in metabolic pathways, especially those of some key enzymes that affect substance metabolism.
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spelling A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sproutsUV irradiationpea sproutsRNA-seqdifferentially expressed genesAbstract Pea sprouts are derived from sprouted peas and are popular for their high vitamin C and flavonoid content, as well as their rapid growth and ease of production. Enhancing its nutritional value with simple treatments is worth exploring, In the present study, we applied supplemental UV irradiation to pea sprouts and examined various components such as vitamin C and flavonoid substances in the treated pea sprouts. Meanwhile, to investigate the mechanism of action of UV irradiation on pea sprouts, we performed high-throughput RNA sequencing (RNA-Seq) analysis to probe the genome-wide expression profile of pea sprouts under UV irradiation, generating 41.90 Gb of Clean Data, the results were also validated by reverse transcription-polymerase chain reaction (RT-qPCR), and the raw data of sequencing were submitted to the SRA repository. Further transcriptome analysis showed that UV irradiation significantly affected the expression of genes in metabolic pathways, especially those of some key enzymes that affect substance metabolism.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101099Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.09022info:eu-repo/semantics/openAccessXU,Ying-haoFU,XinLU,MingWEI,Bao-dongeng2022-04-29T00:00:00Zoai:scielo:S0101-20612022000101099Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-04-29T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
title A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
spellingShingle A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
XU,Ying-hao
UV irradiation
pea sprouts
RNA-seq
differentially expressed genes
title_short A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
title_full A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
title_fullStr A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
title_full_unstemmed A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
title_sort A transcriptomic perspective on the effect of UV irradiation on vitamin C content in pea sprouts
author XU,Ying-hao
author_facet XU,Ying-hao
FU,Xin
LU,Ming
WEI,Bao-dong
author_role author
author2 FU,Xin
LU,Ming
WEI,Bao-dong
author2_role author
author
author
dc.contributor.author.fl_str_mv XU,Ying-hao
FU,Xin
LU,Ming
WEI,Bao-dong
dc.subject.por.fl_str_mv UV irradiation
pea sprouts
RNA-seq
differentially expressed genes
topic UV irradiation
pea sprouts
RNA-seq
differentially expressed genes
description Abstract Pea sprouts are derived from sprouted peas and are popular for their high vitamin C and flavonoid content, as well as their rapid growth and ease of production. Enhancing its nutritional value with simple treatments is worth exploring, In the present study, we applied supplemental UV irradiation to pea sprouts and examined various components such as vitamin C and flavonoid substances in the treated pea sprouts. Meanwhile, to investigate the mechanism of action of UV irradiation on pea sprouts, we performed high-throughput RNA sequencing (RNA-Seq) analysis to probe the genome-wide expression profile of pea sprouts under UV irradiation, generating 41.90 Gb of Clean Data, the results were also validated by reverse transcription-polymerase chain reaction (RT-qPCR), and the raw data of sequencing were submitted to the SRA repository. Further transcriptome analysis showed that UV irradiation significantly affected the expression of genes in metabolic pathways, especially those of some key enzymes that affect substance metabolism.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101099
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.09022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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