Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101001 |
Resumo: | Abstract Present study aims to establish a reliable infant digestion model using pH-STAT method and the model is also applied to static in-vitro digestion of different infant formulas. Model-3 which consisted of NaCl, KCl and CaCl2 as gastrointestinal digestive fluids demonstrated better repeatability than model-1 was selected for the evaluation of static in-vitro digestion of different infant formulas (different fatty acids, protein composition and with/without milk fat globule membrane) using pH-STAT method. Three infant formulas (IF) with different fatty acids composition were evaluated and IF2 which contained the highest amounts of medium-chain saturated fatty acids (8.02 ± 0.47%) demonstrated the highest in-vitro gastrointestinal lipolysis rate (65.88 ± 0.24%). Infant formulas containing peptides and amino acids were more easily hydrolyzed by protease, which probably enhanced the release of FFA (IF1, 50.60 ± 0.87%; IF4, 60.17 ± 2.97%; IF5, 62.21 ± 2.27%; IF6, 66.51 ± 2.38%). In addition, the presence of milk fat globule membrane (MFGM, IF7) was found to accelerate in-vitro gastrointestinal lipolysis (IF1, 50.60 ± 0.87%; IF7, 58.05 ± 1.77%). In summary, the new developed model is suitable for investigating in-vitro digestion of different infant formulas. |
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Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formulapH-STATsimulated digestive fluidsin-vitro gastrointestinal lipolysisfatty acid compositionprotein compositionMFGMAbstract Present study aims to establish a reliable infant digestion model using pH-STAT method and the model is also applied to static in-vitro digestion of different infant formulas. Model-3 which consisted of NaCl, KCl and CaCl2 as gastrointestinal digestive fluids demonstrated better repeatability than model-1 was selected for the evaluation of static in-vitro digestion of different infant formulas (different fatty acids, protein composition and with/without milk fat globule membrane) using pH-STAT method. Three infant formulas (IF) with different fatty acids composition were evaluated and IF2 which contained the highest amounts of medium-chain saturated fatty acids (8.02 ± 0.47%) demonstrated the highest in-vitro gastrointestinal lipolysis rate (65.88 ± 0.24%). Infant formulas containing peptides and amino acids were more easily hydrolyzed by protease, which probably enhanced the release of FFA (IF1, 50.60 ± 0.87%; IF4, 60.17 ± 2.97%; IF5, 62.21 ± 2.27%; IF6, 66.51 ± 2.38%). In addition, the presence of milk fat globule membrane (MFGM, IF7) was found to accelerate in-vitro gastrointestinal lipolysis (IF1, 50.60 ± 0.87%; IF7, 58.05 ± 1.77%). In summary, the new developed model is suitable for investigating in-vitro digestion of different infant formulas.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101001Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.115221info:eu-repo/semantics/openAccessWANG,MengxunXU,ShixiangCHEONG,Ling-ZhiXU,XuebingBI,YanlanZHANG,Hongeng2022-03-15T00:00:00Zoai:scielo:S0101-20612022000101001Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-03-15T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
title |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
spellingShingle |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula WANG,Mengxun pH-STAT simulated digestive fluids in-vitro gastrointestinal lipolysis fatty acid composition protein composition MFGM |
title_short |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
title_full |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
title_fullStr |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
title_full_unstemmed |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
title_sort |
Development of a reliable pH-STAT in-vitro model for gastrointestinal digestion of lipids and application for infant formula |
author |
WANG,Mengxun |
author_facet |
WANG,Mengxun XU,Shixiang CHEONG,Ling-Zhi XU,Xuebing BI,Yanlan ZHANG,Hong |
author_role |
author |
author2 |
XU,Shixiang CHEONG,Ling-Zhi XU,Xuebing BI,Yanlan ZHANG,Hong |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
WANG,Mengxun XU,Shixiang CHEONG,Ling-Zhi XU,Xuebing BI,Yanlan ZHANG,Hong |
dc.subject.por.fl_str_mv |
pH-STAT simulated digestive fluids in-vitro gastrointestinal lipolysis fatty acid composition protein composition MFGM |
topic |
pH-STAT simulated digestive fluids in-vitro gastrointestinal lipolysis fatty acid composition protein composition MFGM |
description |
Abstract Present study aims to establish a reliable infant digestion model using pH-STAT method and the model is also applied to static in-vitro digestion of different infant formulas. Model-3 which consisted of NaCl, KCl and CaCl2 as gastrointestinal digestive fluids demonstrated better repeatability than model-1 was selected for the evaluation of static in-vitro digestion of different infant formulas (different fatty acids, protein composition and with/without milk fat globule membrane) using pH-STAT method. Three infant formulas (IF) with different fatty acids composition were evaluated and IF2 which contained the highest amounts of medium-chain saturated fatty acids (8.02 ± 0.47%) demonstrated the highest in-vitro gastrointestinal lipolysis rate (65.88 ± 0.24%). Infant formulas containing peptides and amino acids were more easily hydrolyzed by protease, which probably enhanced the release of FFA (IF1, 50.60 ± 0.87%; IF4, 60.17 ± 2.97%; IF5, 62.21 ± 2.27%; IF6, 66.51 ± 2.38%). In addition, the presence of milk fat globule membrane (MFGM, IF7) was found to accelerate in-vitro gastrointestinal lipolysis (IF1, 50.60 ± 0.87%; IF7, 58.05 ± 1.77%). In summary, the new developed model is suitable for investigating in-vitro digestion of different infant formulas. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.115221 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126333758996480 |