Homogenate extraction of crude polysaccharides from Lentinus edodes and evaluation of the antioxidant activity

• Autor(a) principal: KE,Leqin
• Data de Publicação: 2016
• Outros Autores: CHEN,Haiyan
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Food Science and Technology (Campinas)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000300533
• topic: crude polysaccharides; Lentinus edodes; homogenate extraction; response surface methodology

Abstract Crude polysaccharides of Lentinus edodes were extracted using homogenate method. Factors affecting the yield of crude polysaccharides were investigated and optimized by response surface methodology. The homogenate extraction method was compared with traditional heating extraction method. The antioxidant activity of crude polysaccharides from Lentinus edodes was evaluated. Results showed that, the optimal conditions of homogenate extraction were as follows: solvent pH, 10; liquid-solid ratio, 30: 1 (mL: g), extraction time, 66 s; number of extraction, 1. Under these conditions, the yield of crude polysaccharides was (13.2 ± 0.9)%, which was 29.82% higher than that of traditional heating extraction. Crude polysaccharides of Lentinus edodes had good DPPH scavenging activity. Compared with the traditional heating extraction, the homogenate extraction had notable advantages including good extraction yield, short extraction time and low extraction temperature. It is an efficient way to extract crude polysaccharides from Lentinus edodes.