Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419 |
Resumo: | Abstract This study investigated the anticarcinogenic effect of lemongrass extract on human SW1417 colon cancer cells. SW1417 cells were cultured and allocated into five groups to apply the treatment with the lemongrass extract using 5 separate fluctuated concentrations: 0, 50, 100, 150 and 200 μg/mL for 24 h. The mRNA expressions of the oxidative pressure genes (HO-1 and NQO1) were measured using the RT-PCR technique. Additionally, mitochondrial morphology was evaluated using Biotium (100 nM MitoView™ 405-Blue) within a free culture. The antitumor activity value (IC50) in SW1417 cells was done at 150 µg/mL. Besides, the changes of mitochondrial morphology in the treated SW1417 cells at all the concentrations of lemongrass extract were markedly observed as mitochondrial fission, which increased with increasing the concentrations and led to an apoptotic effect. For more validation, mRNA levels of the oxidative pressure genes HO-1 and NQO1 were confirmed the obtained data, HO-1 and NQO1 genes expressions recorded significant (P < 0.05) increase which associated with increasing the concentrations of lemongrass extract standardized to β-actin housekeeping gene and contrasted to untreated cells (0 μg/mL). In conclusion, our findings indicate that lemongrass extract provided an anticarcinogenic action against the cell proliferation of human colon cancer cells. |
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Food Science and Technology (Campinas) |
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Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417lemongrass (Cymbopogon citratus STAPF) extracthuman colon cancer cells (SW1417)anticancerapoptosismitochondrial fissionAbstract This study investigated the anticarcinogenic effect of lemongrass extract on human SW1417 colon cancer cells. SW1417 cells were cultured and allocated into five groups to apply the treatment with the lemongrass extract using 5 separate fluctuated concentrations: 0, 50, 100, 150 and 200 μg/mL for 24 h. The mRNA expressions of the oxidative pressure genes (HO-1 and NQO1) were measured using the RT-PCR technique. Additionally, mitochondrial morphology was evaluated using Biotium (100 nM MitoView™ 405-Blue) within a free culture. The antitumor activity value (IC50) in SW1417 cells was done at 150 µg/mL. Besides, the changes of mitochondrial morphology in the treated SW1417 cells at all the concentrations of lemongrass extract were markedly observed as mitochondrial fission, which increased with increasing the concentrations and led to an apoptotic effect. For more validation, mRNA levels of the oxidative pressure genes HO-1 and NQO1 were confirmed the obtained data, HO-1 and NQO1 genes expressions recorded significant (P < 0.05) increase which associated with increasing the concentrations of lemongrass extract standardized to β-actin housekeeping gene and contrasted to untreated cells (0 μg/mL). In conclusion, our findings indicate that lemongrass extract provided an anticarcinogenic action against the cell proliferation of human colon cancer cells.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2023-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419Food Science and Technology v.43 2023reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.94522info:eu-repo/semantics/openAccessALWAILI,Maha Abdullaeng2022-11-16T00:00:00Zoai:scielo:S0101-20612023000100419Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-11-16T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
title |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
spellingShingle |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 ALWAILI,Maha Abdulla lemongrass (Cymbopogon citratus STAPF) extract human colon cancer cells (SW1417) anticancer apoptosis mitochondrial fission |
title_short |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
title_full |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
title_fullStr |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
title_full_unstemmed |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
title_sort |
Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417 |
author |
ALWAILI,Maha Abdulla |
author_facet |
ALWAILI,Maha Abdulla |
author_role |
author |
dc.contributor.author.fl_str_mv |
ALWAILI,Maha Abdulla |
dc.subject.por.fl_str_mv |
lemongrass (Cymbopogon citratus STAPF) extract human colon cancer cells (SW1417) anticancer apoptosis mitochondrial fission |
topic |
lemongrass (Cymbopogon citratus STAPF) extract human colon cancer cells (SW1417) anticancer apoptosis mitochondrial fission |
description |
Abstract This study investigated the anticarcinogenic effect of lemongrass extract on human SW1417 colon cancer cells. SW1417 cells were cultured and allocated into five groups to apply the treatment with the lemongrass extract using 5 separate fluctuated concentrations: 0, 50, 100, 150 and 200 μg/mL for 24 h. The mRNA expressions of the oxidative pressure genes (HO-1 and NQO1) were measured using the RT-PCR technique. Additionally, mitochondrial morphology was evaluated using Biotium (100 nM MitoView™ 405-Blue) within a free culture. The antitumor activity value (IC50) in SW1417 cells was done at 150 µg/mL. Besides, the changes of mitochondrial morphology in the treated SW1417 cells at all the concentrations of lemongrass extract were markedly observed as mitochondrial fission, which increased with increasing the concentrations and led to an apoptotic effect. For more validation, mRNA levels of the oxidative pressure genes HO-1 and NQO1 were confirmed the obtained data, HO-1 and NQO1 genes expressions recorded significant (P < 0.05) increase which associated with increasing the concentrations of lemongrass extract standardized to β-actin housekeeping gene and contrasted to untreated cells (0 μg/mL). In conclusion, our findings indicate that lemongrass extract provided an anticarcinogenic action against the cell proliferation of human colon cancer cells. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.94522 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.43 2023 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126336092078080 |