Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417

Detalhes bibliográficos
Autor(a) principal: ALWAILI,Maha Abdulla
Data de Publicação: 2023
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419
Resumo: Abstract This study investigated the anticarcinogenic effect of lemongrass extract on human SW1417 colon cancer cells. SW1417 cells were cultured and allocated into five groups to apply the treatment with the lemongrass extract using 5 separate fluctuated concentrations: 0, 50, 100, 150 and 200 μg/mL for 24 h. The mRNA expressions of the oxidative pressure genes (HO-1 and NQO1) were measured using the RT-PCR technique. Additionally, mitochondrial morphology was evaluated using Biotium (100 nM MitoView™ 405-Blue) within a free culture. The antitumor activity value (IC50) in SW1417 cells was done at 150 µg/mL. Besides, the changes of mitochondrial morphology in the treated SW1417 cells at all the concentrations of lemongrass extract were markedly observed as mitochondrial fission, which increased with increasing the concentrations and led to an apoptotic effect. For more validation, mRNA levels of the oxidative pressure genes HO-1 and NQO1 were confirmed the obtained data, HO-1 and NQO1 genes expressions recorded significant (P < 0.05) increase which associated with increasing the concentrations of lemongrass extract standardized to β-actin housekeeping gene and contrasted to untreated cells (0 μg/mL). In conclusion, our findings indicate that lemongrass extract provided an anticarcinogenic action against the cell proliferation of human colon cancer cells.
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spelling Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417lemongrass (Cymbopogon citratus STAPF) extracthuman colon cancer cells (SW1417)anticancerapoptosismitochondrial fissionAbstract This study investigated the anticarcinogenic effect of lemongrass extract on human SW1417 colon cancer cells. SW1417 cells were cultured and allocated into five groups to apply the treatment with the lemongrass extract using 5 separate fluctuated concentrations: 0, 50, 100, 150 and 200 μg/mL for 24 h. The mRNA expressions of the oxidative pressure genes (HO-1 and NQO1) were measured using the RT-PCR technique. Additionally, mitochondrial morphology was evaluated using Biotium (100 nM MitoView™ 405-Blue) within a free culture. The antitumor activity value (IC50) in SW1417 cells was done at 150 µg/mL. Besides, the changes of mitochondrial morphology in the treated SW1417 cells at all the concentrations of lemongrass extract were markedly observed as mitochondrial fission, which increased with increasing the concentrations and led to an apoptotic effect. For more validation, mRNA levels of the oxidative pressure genes HO-1 and NQO1 were confirmed the obtained data, HO-1 and NQO1 genes expressions recorded significant (P < 0.05) increase which associated with increasing the concentrations of lemongrass extract standardized to β-actin housekeeping gene and contrasted to untreated cells (0 μg/mL). In conclusion, our findings indicate that lemongrass extract provided an anticarcinogenic action against the cell proliferation of human colon cancer cells.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2023-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419Food Science and Technology v.43 2023reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.94522info:eu-repo/semantics/openAccessALWAILI,Maha Abdullaeng2022-11-16T00:00:00Zoai:scielo:S0101-20612023000100419Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-11-16T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
title Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
spellingShingle Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
ALWAILI,Maha Abdulla
lemongrass (Cymbopogon citratus STAPF) extract
human colon cancer cells (SW1417)
anticancer
apoptosis
mitochondrial fission
title_short Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
title_full Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
title_fullStr Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
title_full_unstemmed Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
title_sort Protective effects of lemongrass (Cymbopogon citratus STAPF) extract mediated mitochondrial fission and glucose uptake inhibition in SW1417
author ALWAILI,Maha Abdulla
author_facet ALWAILI,Maha Abdulla
author_role author
dc.contributor.author.fl_str_mv ALWAILI,Maha Abdulla
dc.subject.por.fl_str_mv lemongrass (Cymbopogon citratus STAPF) extract
human colon cancer cells (SW1417)
anticancer
apoptosis
mitochondrial fission
topic lemongrass (Cymbopogon citratus STAPF) extract
human colon cancer cells (SW1417)
anticancer
apoptosis
mitochondrial fission
description Abstract This study investigated the anticarcinogenic effect of lemongrass extract on human SW1417 colon cancer cells. SW1417 cells were cultured and allocated into five groups to apply the treatment with the lemongrass extract using 5 separate fluctuated concentrations: 0, 50, 100, 150 and 200 μg/mL for 24 h. The mRNA expressions of the oxidative pressure genes (HO-1 and NQO1) were measured using the RT-PCR technique. Additionally, mitochondrial morphology was evaluated using Biotium (100 nM MitoView™ 405-Blue) within a free culture. The antitumor activity value (IC50) in SW1417 cells was done at 150 µg/mL. Besides, the changes of mitochondrial morphology in the treated SW1417 cells at all the concentrations of lemongrass extract were markedly observed as mitochondrial fission, which increased with increasing the concentrations and led to an apoptotic effect. For more validation, mRNA levels of the oxidative pressure genes HO-1 and NQO1 were confirmed the obtained data, HO-1 and NQO1 genes expressions recorded significant (P < 0.05) increase which associated with increasing the concentrations of lemongrass extract standardized to β-actin housekeeping gene and contrasted to untreated cells (0 μg/mL). In conclusion, our findings indicate that lemongrass extract provided an anticarcinogenic action against the cell proliferation of human colon cancer cells.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612023000100419
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.94522
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.43 2023
reponame:Food Science and Technology (Campinas)
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repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
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