Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury

Detalhes bibliográficos
Autor(a) principal: WANG,Fei-Di
Data de Publicação: 2022
Outros Autores: LI,Jie, ZHAI,Xu, CHEN,Rui, WANG,Fang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100740
Resumo: Abstract Clinically, methane is an anti-inflammatory gas used to treat organ damage. There is no extensive research on methane's repair of traumatic brain injury (TBI), so this study aims to investigate the role of methane and its potential mechanism. A rat TBI model was established by mechanical impact. SD rats were divided into four groups, including sham group, TBI group, 2 or 20 mL/kg methane-rich saline (MS) group. Morris water maze test revealed that MS improved cognitive function in TBI rats. HE and Nissl staining showed that MS reduced the blurring of brain tissues boundary, the number of necrotic plaques and neurons in TBI rats. In addition, ELISA revealed that MS restored the oxidative stress of TBI rats and reversed the inflammatory effect of TBI on brain cells, and TUNEL staining detected that MS inhibited cell apoptosis. Western blotting showed that MS downregulated Caspase-3 and Bax protein levels and upregulated Bcl-2 level. And MS inhibited phosphorylated expressions of Janus kinase 1 (JAK1), signal transducer and activator of the transcription (STAT1) and NF-κb-p65. In conclusion, MS improved cognitive function, reduced inflammatory response and oxidative stress and inhibited cell apoptosis to relieve rat TBI by blocking the activation of the JAK1/STAT1/NF-κb-p65 pathway.
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spelling Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injurymethanetraumatic brain injuryinflammationcell apoptosisthe JAK1/STAT1/NF-κB-p65 pathwayAbstract Clinically, methane is an anti-inflammatory gas used to treat organ damage. There is no extensive research on methane's repair of traumatic brain injury (TBI), so this study aims to investigate the role of methane and its potential mechanism. A rat TBI model was established by mechanical impact. SD rats were divided into four groups, including sham group, TBI group, 2 or 20 mL/kg methane-rich saline (MS) group. Morris water maze test revealed that MS improved cognitive function in TBI rats. HE and Nissl staining showed that MS reduced the blurring of brain tissues boundary, the number of necrotic plaques and neurons in TBI rats. In addition, ELISA revealed that MS restored the oxidative stress of TBI rats and reversed the inflammatory effect of TBI on brain cells, and TUNEL staining detected that MS inhibited cell apoptosis. Western blotting showed that MS downregulated Caspase-3 and Bax protein levels and upregulated Bcl-2 level. And MS inhibited phosphorylated expressions of Janus kinase 1 (JAK1), signal transducer and activator of the transcription (STAT1) and NF-κb-p65. In conclusion, MS improved cognitive function, reduced inflammatory response and oxidative stress and inhibited cell apoptosis to relieve rat TBI by blocking the activation of the JAK1/STAT1/NF-κb-p65 pathway.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100740Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.54921info:eu-repo/semantics/openAccessWANG,Fei-DiLI,JieZHAI,XuCHEN,RuiWANG,Fangeng2022-02-23T00:00:00Zoai:scielo:S0101-20612022000100740Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-02-23T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
title Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
spellingShingle Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
WANG,Fei-Di
methane
traumatic brain injury
inflammation
cell apoptosis
the JAK1/STAT1/NF-κB-p65 pathway
title_short Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
title_full Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
title_fullStr Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
title_full_unstemmed Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
title_sort Methane-rich saline restores brain SOD activity and alleviates cognitive impairment in rats with traumatic brain injury
author WANG,Fei-Di
author_facet WANG,Fei-Di
LI,Jie
ZHAI,Xu
CHEN,Rui
WANG,Fang
author_role author
author2 LI,Jie
ZHAI,Xu
CHEN,Rui
WANG,Fang
author2_role author
author
author
author
dc.contributor.author.fl_str_mv WANG,Fei-Di
LI,Jie
ZHAI,Xu
CHEN,Rui
WANG,Fang
dc.subject.por.fl_str_mv methane
traumatic brain injury
inflammation
cell apoptosis
the JAK1/STAT1/NF-κB-p65 pathway
topic methane
traumatic brain injury
inflammation
cell apoptosis
the JAK1/STAT1/NF-κB-p65 pathway
description Abstract Clinically, methane is an anti-inflammatory gas used to treat organ damage. There is no extensive research on methane's repair of traumatic brain injury (TBI), so this study aims to investigate the role of methane and its potential mechanism. A rat TBI model was established by mechanical impact. SD rats were divided into four groups, including sham group, TBI group, 2 or 20 mL/kg methane-rich saline (MS) group. Morris water maze test revealed that MS improved cognitive function in TBI rats. HE and Nissl staining showed that MS reduced the blurring of brain tissues boundary, the number of necrotic plaques and neurons in TBI rats. In addition, ELISA revealed that MS restored the oxidative stress of TBI rats and reversed the inflammatory effect of TBI on brain cells, and TUNEL staining detected that MS inhibited cell apoptosis. Western blotting showed that MS downregulated Caspase-3 and Bax protein levels and upregulated Bcl-2 level. And MS inhibited phosphorylated expressions of Janus kinase 1 (JAK1), signal transducer and activator of the transcription (STAT1) and NF-κb-p65. In conclusion, MS improved cognitive function, reduced inflammatory response and oxidative stress and inhibited cell apoptosis to relieve rat TBI by blocking the activation of the JAK1/STAT1/NF-κb-p65 pathway.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100740
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.54921
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
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reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
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