The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill

Detalhes bibliográficos
Autor(a) principal: LI,Tiejun
Data de Publicação: 2022
Outros Autores: YANG,Chenghu, HU,Hongmei, ZHANG,Bo, MA,Limin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100517
Resumo: Abstract To explore the gene expression profiling in gazami crab’s hepatopancreas and gill tissues which were challenged with CuO-NPs. Specimens of gazami crab P. trituberculatus were collected and challenged with CuO-NPs, then their hepatopancreas and gill tissues were dissected for RNA extraction. The cDNA libraries were synthesized and sequenced. De novo assembly of crab transcriptome was conducted for gene expression quantification and differential expression analysis. Finally the results were validated by RT-PCR. 56 unigenes displayed differential expression pattern in CuO-NPs treated hepatopancreas tissue. and 273 unigenes displayed differential expression pattern in CuO-NPs treated gill tissue. The gene expression pattern between control and CuO-NPs treated hepatopancreas was very closed to each other, while the gene expression pattern between control and CuO-NPs treated gill was more distinct. 13 genes were mutually exclusive to participate, and he data generated from RNAseq was well consistent with those obtained from RT-PCR. CuO-NPs could induce toxic effects in crab gills as well as in genetic level and the 13 genes might be the potential marker genes for CuO-NPs toxicology.
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spelling The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gillCuO-NPshepatopancreasgillgazami crabAbstract To explore the gene expression profiling in gazami crab’s hepatopancreas and gill tissues which were challenged with CuO-NPs. Specimens of gazami crab P. trituberculatus were collected and challenged with CuO-NPs, then their hepatopancreas and gill tissues were dissected for RNA extraction. The cDNA libraries were synthesized and sequenced. De novo assembly of crab transcriptome was conducted for gene expression quantification and differential expression analysis. Finally the results were validated by RT-PCR. 56 unigenes displayed differential expression pattern in CuO-NPs treated hepatopancreas tissue. and 273 unigenes displayed differential expression pattern in CuO-NPs treated gill tissue. The gene expression pattern between control and CuO-NPs treated hepatopancreas was very closed to each other, while the gene expression pattern between control and CuO-NPs treated gill was more distinct. 13 genes were mutually exclusive to participate, and he data generated from RNAseq was well consistent with those obtained from RT-PCR. CuO-NPs could induce toxic effects in crab gills as well as in genetic level and the 13 genes might be the potential marker genes for CuO-NPs toxicology.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100517Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.03521info:eu-repo/semantics/openAccessLI,TiejunYANG,ChenghuHU,HongmeiZHANG,BoMA,Limineng2022-02-22T00:00:00Zoai:scielo:S0101-20612022000100517Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-02-22T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
title The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
spellingShingle The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
LI,Tiejun
CuO-NPs
hepatopancreas
gill
gazami crab
title_short The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
title_full The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
title_fullStr The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
title_full_unstemmed The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
title_sort The toxico-transcriptomic analysis of nano-copper oxide on gazami crab: especially focus on hepatopancreas and gill
author LI,Tiejun
author_facet LI,Tiejun
YANG,Chenghu
HU,Hongmei
ZHANG,Bo
MA,Limin
author_role author
author2 YANG,Chenghu
HU,Hongmei
ZHANG,Bo
MA,Limin
author2_role author
author
author
author
dc.contributor.author.fl_str_mv LI,Tiejun
YANG,Chenghu
HU,Hongmei
ZHANG,Bo
MA,Limin
dc.subject.por.fl_str_mv CuO-NPs
hepatopancreas
gill
gazami crab
topic CuO-NPs
hepatopancreas
gill
gazami crab
description Abstract To explore the gene expression profiling in gazami crab’s hepatopancreas and gill tissues which were challenged with CuO-NPs. Specimens of gazami crab P. trituberculatus were collected and challenged with CuO-NPs, then their hepatopancreas and gill tissues were dissected for RNA extraction. The cDNA libraries were synthesized and sequenced. De novo assembly of crab transcriptome was conducted for gene expression quantification and differential expression analysis. Finally the results were validated by RT-PCR. 56 unigenes displayed differential expression pattern in CuO-NPs treated hepatopancreas tissue. and 273 unigenes displayed differential expression pattern in CuO-NPs treated gill tissue. The gene expression pattern between control and CuO-NPs treated hepatopancreas was very closed to each other, while the gene expression pattern between control and CuO-NPs treated gill was more distinct. 13 genes were mutually exclusive to participate, and he data generated from RNAseq was well consistent with those obtained from RT-PCR. CuO-NPs could induce toxic effects in crab gills as well as in genetic level and the 13 genes might be the potential marker genes for CuO-NPs toxicology.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100517
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100517
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.03521
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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