Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells

Detalhes bibliográficos
Autor(a) principal: LIU,Ying
Data de Publicação: 2022
Outros Autores: WU,Min, ZHI,Mingyu, TANG,Ping, KE,Leqin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101082
Resumo: Abstract In this study, the total triterpenoids of raspberry fruit (TTRF) were prepared, and their effects on human hepatocellular carcinoma SMMC-7721 cells were investigated. SMMC-7721 cells were treated with TTRF with concentration of 0 (control), 50, 100, 200 and 400 μg/mL, respectively. The cell proliferation, cycle and apoptosis were detected. The reactive oxygen species (ROS) in cells and the expression levels of B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-2 associated X (Bax) in cells were determined. Results showed that, TTRF could obviously inhibit the proliferation of SMMC-7721 cells, arrest most cells in S and G2/M phases, and promote their apoptosis. In addition, TTRF could increase the ROS level, down-regulate the Bcl-2 protein expression and up-regulate the Bax protein expression in cells. In conclusion, cucurbitacin I has inhibitory effect on growth of SMMC-7721cells. The action mechanism may be related to its increasing ROS level and regulating of Bcl-2 and Bax expression in cells.
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spelling Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cellstotal triterpenoidsraspberry fruitlive cancerSMMC-7721Abstract In this study, the total triterpenoids of raspberry fruit (TTRF) were prepared, and their effects on human hepatocellular carcinoma SMMC-7721 cells were investigated. SMMC-7721 cells were treated with TTRF with concentration of 0 (control), 50, 100, 200 and 400 μg/mL, respectively. The cell proliferation, cycle and apoptosis were detected. The reactive oxygen species (ROS) in cells and the expression levels of B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-2 associated X (Bax) in cells were determined. Results showed that, TTRF could obviously inhibit the proliferation of SMMC-7721 cells, arrest most cells in S and G2/M phases, and promote their apoptosis. In addition, TTRF could increase the ROS level, down-regulate the Bcl-2 protein expression and up-regulate the Bax protein expression in cells. In conclusion, cucurbitacin I has inhibitory effect on growth of SMMC-7721cells. The action mechanism may be related to its increasing ROS level and regulating of Bcl-2 and Bax expression in cells.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101082Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.16722info:eu-repo/semantics/openAccessLIU,YingWU,MinZHI,MingyuTANG,PingKE,Leqineng2022-04-18T00:00:00Zoai:scielo:S0101-20612022000101082Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-04-18T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
title Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
spellingShingle Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
LIU,Ying
total triterpenoids
raspberry fruit
live cancer
SMMC-7721
title_short Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
title_full Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
title_fullStr Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
title_full_unstemmed Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
title_sort Extraction of total triterpenoids from raspberry fruit and evaluation of their effects on human hepatocellular carcinoma cells
author LIU,Ying
author_facet LIU,Ying
WU,Min
ZHI,Mingyu
TANG,Ping
KE,Leqin
author_role author
author2 WU,Min
ZHI,Mingyu
TANG,Ping
KE,Leqin
author2_role author
author
author
author
dc.contributor.author.fl_str_mv LIU,Ying
WU,Min
ZHI,Mingyu
TANG,Ping
KE,Leqin
dc.subject.por.fl_str_mv total triterpenoids
raspberry fruit
live cancer
SMMC-7721
topic total triterpenoids
raspberry fruit
live cancer
SMMC-7721
description Abstract In this study, the total triterpenoids of raspberry fruit (TTRF) were prepared, and their effects on human hepatocellular carcinoma SMMC-7721 cells were investigated. SMMC-7721 cells were treated with TTRF with concentration of 0 (control), 50, 100, 200 and 400 μg/mL, respectively. The cell proliferation, cycle and apoptosis were detected. The reactive oxygen species (ROS) in cells and the expression levels of B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-2 associated X (Bax) in cells were determined. Results showed that, TTRF could obviously inhibit the proliferation of SMMC-7721 cells, arrest most cells in S and G2/M phases, and promote their apoptosis. In addition, TTRF could increase the ROS level, down-regulate the Bcl-2 protein expression and up-regulate the Bax protein expression in cells. In conclusion, cucurbitacin I has inhibitory effect on growth of SMMC-7721cells. The action mechanism may be related to its increasing ROS level and regulating of Bcl-2 and Bax expression in cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101082
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101082
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.16722
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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