Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

Detalhes bibliográficos
Autor(a) principal: KEYVAN,Erhan
Data de Publicação: 2020
Outros Autores: YURDAKUL,Ozen, DEMIRTAS,Ahu, YALCIN,Halil, BILGEN,Nuket
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100150
Resumo: Abstract Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. One hundred and twenty bulk tank milk samples were analyzed using the convenient sampling method. The isolates were confirmed by real-time polymerase chain reaction (RT-PCR) targeting nuc and mecA gene in S. aureus. Antibiotic resistance profiles of the isolates were tested by disc agar diffusion method. In the current study, S. aureus was detected in 44 (36.66%) of the samples. The presence of the mecA gene was found to be positive in 40 (75.4%) of the 53 S. aureus isolates. As a result of the study, a high incidence of MRSA was detected in bulk tank milk samples. Antibiotic-resistant bacteria are at risk of being transferred to humans via milk. For safe and healthy milk consumption, uncontrolled use of antibiotics in dairy cows should be avoided.
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spelling Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milkantibiotic resistancebulk tank milkMRSAAbstract Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. One hundred and twenty bulk tank milk samples were analyzed using the convenient sampling method. The isolates were confirmed by real-time polymerase chain reaction (RT-PCR) targeting nuc and mecA gene in S. aureus. Antibiotic resistance profiles of the isolates were tested by disc agar diffusion method. In the current study, S. aureus was detected in 44 (36.66%) of the samples. The presence of the mecA gene was found to be positive in 40 (75.4%) of the 53 S. aureus isolates. As a result of the study, a high incidence of MRSA was detected in bulk tank milk samples. Antibiotic-resistant bacteria are at risk of being transferred to humans via milk. For safe and healthy milk consumption, uncontrolled use of antibiotics in dairy cows should be avoided.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2020-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100150Food Science and Technology v.40 n.1 2020reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.35818info:eu-repo/semantics/openAccessKEYVAN,ErhanYURDAKUL,OzenDEMIRTAS,AhuYALCIN,HalilBILGEN,Nuketeng2020-05-29T00:00:00Zoai:scielo:S0101-20612020000100150Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2020-05-29T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
title Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
spellingShingle Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
KEYVAN,Erhan
antibiotic resistance
bulk tank milk
MRSA
title_short Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
title_full Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
title_fullStr Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
title_full_unstemmed Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
title_sort Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
author KEYVAN,Erhan
author_facet KEYVAN,Erhan
YURDAKUL,Ozen
DEMIRTAS,Ahu
YALCIN,Halil
BILGEN,Nuket
author_role author
author2 YURDAKUL,Ozen
DEMIRTAS,Ahu
YALCIN,Halil
BILGEN,Nuket
author2_role author
author
author
author
dc.contributor.author.fl_str_mv KEYVAN,Erhan
YURDAKUL,Ozen
DEMIRTAS,Ahu
YALCIN,Halil
BILGEN,Nuket
dc.subject.por.fl_str_mv antibiotic resistance
bulk tank milk
MRSA
topic antibiotic resistance
bulk tank milk
MRSA
description Abstract Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. One hundred and twenty bulk tank milk samples were analyzed using the convenient sampling method. The isolates were confirmed by real-time polymerase chain reaction (RT-PCR) targeting nuc and mecA gene in S. aureus. Antibiotic resistance profiles of the isolates were tested by disc agar diffusion method. In the current study, S. aureus was detected in 44 (36.66%) of the samples. The presence of the mecA gene was found to be positive in 40 (75.4%) of the 53 S. aureus isolates. As a result of the study, a high incidence of MRSA was detected in bulk tank milk samples. Antibiotic-resistant bacteria are at risk of being transferred to humans via milk. For safe and healthy milk consumption, uncontrolled use of antibiotics in dairy cows should be avoided.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100150
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100150
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.35818
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.40 n.1 2020
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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