Photodynamic action of the red laser on Propionibacterium acnes

• Main Author: Ramos,Rogério Rodrigo
• Publication Date: 2017
• Other Authors: Paiva,Jeferson Leandro de, Gomes,José Paulo Franco dos Santos, Boer,Nagib Pezati, Godoy,José Maria Pereira de, Batigalia,Fernando
• Format: Article
• Language: eng
• Source: Anais brasileiros de dermatologia (Online)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962017000500622
• topic: Laser therapy; Methylene blue; Propionibacterium acnes

Abstract: Background: Photodynamic therapy is a therapeutic modality that has consolidated its activity in the photooxidation of organic matter, which arises from the activity of reactive oxygen species. Objective: To evaluate the effect of red laser 660nm with the photosensitizer methylene blue on Propionibacterium acnes in vitro. Method: The experimental design was distributed into four groups (1 - control group without the application of light and without photosensitizer, 2 - application of light, 3 - methylene blue without light, and 4 - methylene blue with light). Tests were subjected to red laser irradiation 660nm by four cycles of 5 minutes at 3-minute intervals. Results: It was evidenced the prominence of the fourth cycle (20 minutes) groups 2, 3 and 4. Study limitations: Despite the favorable results, the laser irradiation time photosensitizer associated with methylene blue were not sufficient to to completely inhibit the proliferation of bacteria. Conclusion: Further studies in vitro are recommended to enable the clinical application of this photosensitizer in photodynamic therapy.