High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris?
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais brasileiros de dermatologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962022000600729 |
Resumo: | Abstract Background: Acne vulgaris is an inflammatory skin disorder leading to an impairment of quality of life and is therefore not only a cosmetic issue. Its pathogenesis is multifactorial - of particular importance is the colonization with the bacterium Propionibacterium acnes. A wide range of different treatment options exists including topical and systemic treatments depending on severity. High Frequency (HF) therapy, historically developed in the 19th century, claims antimicrobial effects on acne skin, but solid data on its efficacy and mechanism of action is lacking. Objective: The main objective of this study was to determine the efficacy of HF therapy on skin flora and P. acnes in vitro using a commercial device as well as to review studies on the mechanism of action. Methods: The plasma source was investigated regarding electrical settings, heat, and ozone development. Bacterial skin flora, fungal isolates, and P. acnes were exposed to HF in vitro and compared to unexposed controls by evaluating the number of colonies on agar plates. To further analyze bacterial species from normal skin flora, 16S-sequencing was performed. Statistical analyses were carried out using row analysis and unpaired t-test. Results: HF treatment led to a significant reduction of almost every bacterial and fungal species investigated in this study. Moreover, the number of colonies forming units was significantly decreased in P. acnes after HF treatment compared to controls in vitro. Study limitations: The experiments were performed in vitro only. To assess clinical effects further in vivo experiments are necessary. Conclusions: The results collected in this study, although in vitro, provide a mechanistic basis for HF as a complementary treatment option for patients with acne. It might also have a beneficial effect on patients with superficial infectious skin of the skin. |
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Anais brasileiros de dermatologia (Online) |
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High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris?Acne vulgarisBacterial infectionHigh-frequencyMicrobiomeAbstract Background: Acne vulgaris is an inflammatory skin disorder leading to an impairment of quality of life and is therefore not only a cosmetic issue. Its pathogenesis is multifactorial - of particular importance is the colonization with the bacterium Propionibacterium acnes. A wide range of different treatment options exists including topical and systemic treatments depending on severity. High Frequency (HF) therapy, historically developed in the 19th century, claims antimicrobial effects on acne skin, but solid data on its efficacy and mechanism of action is lacking. Objective: The main objective of this study was to determine the efficacy of HF therapy on skin flora and P. acnes in vitro using a commercial device as well as to review studies on the mechanism of action. Methods: The plasma source was investigated regarding electrical settings, heat, and ozone development. Bacterial skin flora, fungal isolates, and P. acnes were exposed to HF in vitro and compared to unexposed controls by evaluating the number of colonies on agar plates. To further analyze bacterial species from normal skin flora, 16S-sequencing was performed. Statistical analyses were carried out using row analysis and unpaired t-test. Results: HF treatment led to a significant reduction of almost every bacterial and fungal species investigated in this study. Moreover, the number of colonies forming units was significantly decreased in P. acnes after HF treatment compared to controls in vitro. Study limitations: The experiments were performed in vitro only. To assess clinical effects further in vivo experiments are necessary. Conclusions: The results collected in this study, although in vitro, provide a mechanistic basis for HF as a complementary treatment option for patients with acne. It might also have a beneficial effect on patients with superficial infectious skin of the skin.Sociedade Brasileira de Dermatologia2022-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962022000600729Anais Brasileiros de Dermatologia v.97 n.6 2022reponame:Anais brasileiros de dermatologia (Online)instname:Sociedade Brasileira de Dermatologia (SBD)instacron:SBD10.1016/j.abd.2021.09.015info:eu-repo/semantics/openAccessFrommherz,LeonieReinholz,MarkusGürtler,AnneStadler,Pia-CharlotteKaemmerer,TillFrench,LarsClanner-Engelshofen,Benjamin M.eng2022-11-10T00:00:00Zoai:scielo:S0365-05962022000600729Revistahttp://www.anaisdedermatologia.org.br/https://old.scielo.br/oai/scielo-oai.phpabd@sbd.org.br||revista@sbd.org.br1806-48410365-0596opendoar:2022-11-10T00:00Anais brasileiros de dermatologia (Online) - Sociedade Brasileira de Dermatologia (SBD)false |
dc.title.none.fl_str_mv |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
title |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
spellingShingle |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? Frommherz,Leonie Acne vulgaris Bacterial infection High-frequency Microbiome |
title_short |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
title_full |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
title_fullStr |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
title_full_unstemmed |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
title_sort |
High-frequency devices effect in vitro: promissing approach in the treatment of acne vulgaris? |
author |
Frommherz,Leonie |
author_facet |
Frommherz,Leonie Reinholz,Markus Gürtler,Anne Stadler,Pia-Charlotte Kaemmerer,Till French,Lars Clanner-Engelshofen,Benjamin M. |
author_role |
author |
author2 |
Reinholz,Markus Gürtler,Anne Stadler,Pia-Charlotte Kaemmerer,Till French,Lars Clanner-Engelshofen,Benjamin M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Frommherz,Leonie Reinholz,Markus Gürtler,Anne Stadler,Pia-Charlotte Kaemmerer,Till French,Lars Clanner-Engelshofen,Benjamin M. |
dc.subject.por.fl_str_mv |
Acne vulgaris Bacterial infection High-frequency Microbiome |
topic |
Acne vulgaris Bacterial infection High-frequency Microbiome |
description |
Abstract Background: Acne vulgaris is an inflammatory skin disorder leading to an impairment of quality of life and is therefore not only a cosmetic issue. Its pathogenesis is multifactorial - of particular importance is the colonization with the bacterium Propionibacterium acnes. A wide range of different treatment options exists including topical and systemic treatments depending on severity. High Frequency (HF) therapy, historically developed in the 19th century, claims antimicrobial effects on acne skin, but solid data on its efficacy and mechanism of action is lacking. Objective: The main objective of this study was to determine the efficacy of HF therapy on skin flora and P. acnes in vitro using a commercial device as well as to review studies on the mechanism of action. Methods: The plasma source was investigated regarding electrical settings, heat, and ozone development. Bacterial skin flora, fungal isolates, and P. acnes were exposed to HF in vitro and compared to unexposed controls by evaluating the number of colonies on agar plates. To further analyze bacterial species from normal skin flora, 16S-sequencing was performed. Statistical analyses were carried out using row analysis and unpaired t-test. Results: HF treatment led to a significant reduction of almost every bacterial and fungal species investigated in this study. Moreover, the number of colonies forming units was significantly decreased in P. acnes after HF treatment compared to controls in vitro. Study limitations: The experiments were performed in vitro only. To assess clinical effects further in vivo experiments are necessary. Conclusions: The results collected in this study, although in vitro, provide a mechanistic basis for HF as a complementary treatment option for patients with acne. It might also have a beneficial effect on patients with superficial infectious skin of the skin. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962022000600729 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962022000600729 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.abd.2021.09.015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Dermatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Dermatologia |
dc.source.none.fl_str_mv |
Anais Brasileiros de Dermatologia v.97 n.6 2022 reponame:Anais brasileiros de dermatologia (Online) instname:Sociedade Brasileira de Dermatologia (SBD) instacron:SBD |
instname_str |
Sociedade Brasileira de Dermatologia (SBD) |
instacron_str |
SBD |
institution |
SBD |
reponame_str |
Anais brasileiros de dermatologia (Online) |
collection |
Anais brasileiros de dermatologia (Online) |
repository.name.fl_str_mv |
Anais brasileiros de dermatologia (Online) - Sociedade Brasileira de Dermatologia (SBD) |
repository.mail.fl_str_mv |
abd@sbd.org.br||revista@sbd.org.br |
_version_ |
1752126424983011328 |