Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,

Detalhes bibliográficos
Autor(a) principal: Leite,Marcel Nani
Data de Publicação: 2020
Outros Autores: Leite,Saulo Nani, Caetano,Guilherme Ferreira, Andrade,Thiago Antônio Moretti de, Fronza,Márcio, Frade,Marco Andrey Cipriani
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais brasileiros de dermatologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962020000400002
Resumo: Abstract Background Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. Objective This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. Methods For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. Results Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitations Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. Conclusion In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.
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spelling Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,DermabrasionInflammationLatexWound healingAbstract Background Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. Objective This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. Methods For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. Results Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitations Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. Conclusion In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.Sociedade Brasileira de Dermatologia2020-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962020000400002Anais Brasileiros de Dermatologia v.95 n.4 2020reponame:Anais brasileiros de dermatologia (Online)instname:Sociedade Brasileira de Dermatologia (SBD)instacron:SBD10.1016/j.abd.2019.12.003info:eu-repo/semantics/openAccessLeite,Marcel NaniLeite,Saulo NaniCaetano,Guilherme FerreiraAndrade,Thiago Antônio Moretti deFronza,MárcioFrade,Marco Andrey Ciprianieng2020-08-04T00:00:00Zoai:scielo:S0365-05962020000400002Revistahttp://www.anaisdedermatologia.org.br/https://old.scielo.br/oai/scielo-oai.phpabd@sbd.org.br||revista@sbd.org.br1806-48410365-0596opendoar:2020-08-04T00:00Anais brasileiros de dermatologia (Online) - Sociedade Brasileira de Dermatologia (SBD)false
dc.title.none.fl_str_mv Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
title Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
spellingShingle Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
Leite,Marcel Nani
Dermabrasion
Inflammation
Latex
Wound healing
title_short Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
title_full Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
title_fullStr Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
title_full_unstemmed Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
title_sort Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model,
author Leite,Marcel Nani
author_facet Leite,Marcel Nani
Leite,Saulo Nani
Caetano,Guilherme Ferreira
Andrade,Thiago Antônio Moretti de
Fronza,Márcio
Frade,Marco Andrey Cipriani
author_role author
author2 Leite,Saulo Nani
Caetano,Guilherme Ferreira
Andrade,Thiago Antônio Moretti de
Fronza,Márcio
Frade,Marco Andrey Cipriani
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Leite,Marcel Nani
Leite,Saulo Nani
Caetano,Guilherme Ferreira
Andrade,Thiago Antônio Moretti de
Fronza,Márcio
Frade,Marco Andrey Cipriani
dc.subject.por.fl_str_mv Dermabrasion
Inflammation
Latex
Wound healing
topic Dermabrasion
Inflammation
Latex
Wound healing
description Abstract Background Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. Objective This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. Methods For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. Results Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitations Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. Conclusion In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1016/j.abd.2019.12.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Dermatologia
publisher.none.fl_str_mv Sociedade Brasileira de Dermatologia
dc.source.none.fl_str_mv Anais Brasileiros de Dermatologia v.95 n.4 2020
reponame:Anais brasileiros de dermatologia (Online)
instname:Sociedade Brasileira de Dermatologia (SBD)
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instname_str Sociedade Brasileira de Dermatologia (SBD)
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institution SBD
reponame_str Anais brasileiros de dermatologia (Online)
collection Anais brasileiros de dermatologia (Online)
repository.name.fl_str_mv Anais brasileiros de dermatologia (Online) - Sociedade Brasileira de Dermatologia (SBD)
repository.mail.fl_str_mv abd@sbd.org.br||revista@sbd.org.br
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