Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction

• Autor(a) principal: Alecrim,Hugo Muscelli
• Data de Publicação: 2015
• Outros Autores: Duarte,Sérgio Andurte Carvalho, Amaral,Maria Eduarda Bonavides, Diógenis,Filipe, Carneiro,Fabiana Pirani, Sousa,João Batista de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000100073
• topic: Glutamine; Jaundice; Colon; Wound Healing; Bilirubin; Colorectal Surgery; Rats

PURPOSE:To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats.METHODS: Sixteen male rats were allocated across four groups: LG - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis.RESULTS:There were no differences in bursting pressure among groups : LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation.CONCLUSION: Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats.