Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Detalhes bibliográficos
Autor(a) principal: Ikejiri,Adauto Tsutomu
Data de Publicação: 2014
Outros Autores: Somaio Neto,Frederico, Chaves,José Carlos, Bertoletto,Paulo Roberto, Teruya,Roberto, Bertoletto,Eduardo Rodrigues, Taha,Murched Omar, Fagundes,Djalma José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000300186
Resumo: PURPOSE: To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2
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spelling Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injuryGene ExpressionOxidative StressLungReperfusion InjuryMicePURPOSE: To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000300186Acta Cirúrgica Brasileira v.29 n.3 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014000300007info:eu-repo/semantics/openAccessIkejiri,Adauto TsutomuSomaio Neto,FredericoChaves,José CarlosBertoletto,Paulo RobertoTeruya,RobertoBertoletto,Eduardo RodriguesTaha,Murched OmarFagundes,Djalma Joséeng2015-10-26T00:00:00Zoai:scielo:S0102-86502014000300186Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-26T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
title Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
spellingShingle Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
Ikejiri,Adauto Tsutomu
Gene Expression
Oxidative Stress
Lung
Reperfusion Injury
Mice
title_short Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
title_full Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
title_fullStr Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
title_full_unstemmed Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
title_sort Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
author Ikejiri,Adauto Tsutomu
author_facet Ikejiri,Adauto Tsutomu
Somaio Neto,Frederico
Chaves,José Carlos
Bertoletto,Paulo Roberto
Teruya,Roberto
Bertoletto,Eduardo Rodrigues
Taha,Murched Omar
Fagundes,Djalma José
author_role author
author2 Somaio Neto,Frederico
Chaves,José Carlos
Bertoletto,Paulo Roberto
Teruya,Roberto
Bertoletto,Eduardo Rodrigues
Taha,Murched Omar
Fagundes,Djalma José
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ikejiri,Adauto Tsutomu
Somaio Neto,Frederico
Chaves,José Carlos
Bertoletto,Paulo Roberto
Teruya,Roberto
Bertoletto,Eduardo Rodrigues
Taha,Murched Omar
Fagundes,Djalma José
dc.subject.por.fl_str_mv Gene Expression
Oxidative Stress
Lung
Reperfusion Injury
Mice
topic Gene Expression
Oxidative Stress
Lung
Reperfusion Injury
Mice
description PURPOSE: To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2
publishDate 2014
dc.date.none.fl_str_mv 2014-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000300186
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000300186
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014000300007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 n.3 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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