The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats

Detalhes bibliográficos
Autor(a) principal: Yang,Xuan
Data de Publicação: 2022
Outros Autores: Yu,Hui, Wei,Jingli, Wei,Qiuyan, Huang,Hui, Chen,Jing, Li,Jianzhe, Yu,Shuyi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900203
Resumo: ABSTRACT Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI). Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed. Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics. Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
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spelling The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in ratsClostridium butyricumReperfusion InjuryFatty Acids, VolatileInflammationGut MicrobiotaOxidative StressABSTRACT Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI). Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed. Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics. Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900203Acta Cirúrgica Brasileira v.37 n.9 2022reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/acb370904info:eu-repo/semantics/openAccessYang,XuanYu,HuiWei,JingliWei,QiuyanHuang,HuiChen,JingLi,JianzheYu,Shuyieng2022-12-08T00:00:00Zoai:scielo:S0102-86502022000900203Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2022-12-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
title The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
spellingShingle The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
Yang,Xuan
Clostridium butyricum
Reperfusion Injury
Fatty Acids, Volatile
Inflammation
Gut Microbiota
Oxidative Stress
title_short The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
title_full The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
title_fullStr The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
title_full_unstemmed The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
title_sort The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats
author Yang,Xuan
author_facet Yang,Xuan
Yu,Hui
Wei,Jingli
Wei,Qiuyan
Huang,Hui
Chen,Jing
Li,Jianzhe
Yu,Shuyi
author_role author
author2 Yu,Hui
Wei,Jingli
Wei,Qiuyan
Huang,Hui
Chen,Jing
Li,Jianzhe
Yu,Shuyi
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Yang,Xuan
Yu,Hui
Wei,Jingli
Wei,Qiuyan
Huang,Hui
Chen,Jing
Li,Jianzhe
Yu,Shuyi
dc.subject.por.fl_str_mv Clostridium butyricum
Reperfusion Injury
Fatty Acids, Volatile
Inflammation
Gut Microbiota
Oxidative Stress
topic Clostridium butyricum
Reperfusion Injury
Fatty Acids, Volatile
Inflammation
Gut Microbiota
Oxidative Stress
description ABSTRACT Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI). Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed. Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics. Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900203
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900203
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/acb370904
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.37 n.9 2022
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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