Alveolar osseous defect in rat for cell therapy: preliminary report

Detalhes bibliográficos
Autor(a) principal: Raposo-Amaral,Cassio Eduardo
Data de Publicação: 2010
Outros Autores: Kobayashi,Gerson Shigeru, Almeida,Ana Beatriz, Bueno,Daniela F., Freitas,Fatima Rodrigues de Souza e, Vulcano,Luiz Carlos, Passos-Bueno,Maria Rita, Alonso,Nivaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400002
Resumo: PURPOSE: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. METHODS: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm² of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). RESULTS: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm² and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm². The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm² and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm². Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). CONCLUSION: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats.
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spelling Alveolar osseous defect in rat for cell therapy: preliminary reportAlveolar Bone LossTissue TherapyRatsPURPOSE: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. METHODS: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm² of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). RESULTS: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm² and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm². The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm² and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm². Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). CONCLUSION: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400002Acta Cirúrgica Brasileira v.25 n.4 2010reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502010000400002info:eu-repo/semantics/openAccessRaposo-Amaral,Cassio EduardoKobayashi,Gerson ShigeruAlmeida,Ana BeatrizBueno,Daniela F.Freitas,Fatima Rodrigues de Souza eVulcano,Luiz CarlosPassos-Bueno,Maria RitaAlonso,Nivaldoeng2010-07-26T00:00:00Zoai:scielo:S0102-86502010000400002Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-07-26T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Alveolar osseous defect in rat for cell therapy: preliminary report
title Alveolar osseous defect in rat for cell therapy: preliminary report
spellingShingle Alveolar osseous defect in rat for cell therapy: preliminary report
Raposo-Amaral,Cassio Eduardo
Alveolar Bone Loss
Tissue Therapy
Rats
title_short Alveolar osseous defect in rat for cell therapy: preliminary report
title_full Alveolar osseous defect in rat for cell therapy: preliminary report
title_fullStr Alveolar osseous defect in rat for cell therapy: preliminary report
title_full_unstemmed Alveolar osseous defect in rat for cell therapy: preliminary report
title_sort Alveolar osseous defect in rat for cell therapy: preliminary report
author Raposo-Amaral,Cassio Eduardo
author_facet Raposo-Amaral,Cassio Eduardo
Kobayashi,Gerson Shigeru
Almeida,Ana Beatriz
Bueno,Daniela F.
Freitas,Fatima Rodrigues de Souza e
Vulcano,Luiz Carlos
Passos-Bueno,Maria Rita
Alonso,Nivaldo
author_role author
author2 Kobayashi,Gerson Shigeru
Almeida,Ana Beatriz
Bueno,Daniela F.
Freitas,Fatima Rodrigues de Souza e
Vulcano,Luiz Carlos
Passos-Bueno,Maria Rita
Alonso,Nivaldo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Raposo-Amaral,Cassio Eduardo
Kobayashi,Gerson Shigeru
Almeida,Ana Beatriz
Bueno,Daniela F.
Freitas,Fatima Rodrigues de Souza e
Vulcano,Luiz Carlos
Passos-Bueno,Maria Rita
Alonso,Nivaldo
dc.subject.por.fl_str_mv Alveolar Bone Loss
Tissue Therapy
Rats
topic Alveolar Bone Loss
Tissue Therapy
Rats
description PURPOSE: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. METHODS: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm² of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). RESULTS: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm² and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm². The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm² and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm². Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). CONCLUSION: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000400002
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502010000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.25 n.4 2010
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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