Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye

• Autor(a) principal: Aquino,José Ulcijara
• Data de Publicação: 2012
• Outros Autores: Pinheiro,Luiz Gonzaga Porto, Vasques,Paulo Henrique Diógenes, Rocha,João Ivo Xavier, Cruz,Diego Alves, Beserra,Hugo Enrique Orsini, Cavalcante,Raissa Vasconcelos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000200002
• topic: Vulvar Neoplasms; Sentinel Lymph Node Biopsy; Technetium; Animal Experimentation; Dogs

PURPOSE: This paper aims to study and define the experimental model of sentinel lymph node biopsy of the vulva in bitches. METHODS: 0.2 ml of 99mTc phytate was injected intradermally, using a fine gauge insulin needle in the anterior commissure of the vulva. Thirty minutes after 99mTc injection, the inguinal mapping was performed using a gamma probe. After this, 0.5 ml of blue dye (bleu patenté V Guerbet 2.5%) was injected in the same place. After 15 minutes, a 3 cm long inguinal incision was made at point maximum uptake followed by careful dissection, guided by visualization of a bluish afferent lymphatic system that points to the sentinel lymph node (SLN). RESULTS: It was observed that 88% of SLN were identified. It wasn´t found a significant difference among the presence or not of sentinel lymph node in the sides, which is an indication of a good consistency. It was observed a high (88%) and significant (χ2=12.89 and p=0.0003) intercession between both methods (blue dye and radiation). CONCLUSION: The experimental model adopted is feasible, becoming advantageous in applying the association of Patent blue and 99mTc.