Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia?
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000300001 |
Resumo: | PURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI. |
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Acta Cirúrgica Brasileira (Online) |
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Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia?KidneyIschemiaReperfusionHyperglycemiaPropofolIsofluraneRatsPURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000300001Acta Cirúrgica Brasileira v.28 n.3 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013000300001info:eu-repo/semantics/openAccessCarraretto,Antônio RobertoVianna Filho,Pedro Thadeu GalvãoCastiglia,Yara Marcondes MachadoGolim,Marjorie de AssisSouza,Aparecida Vitória Gonçalves deCarvalho,Lídia Raquel deDeffune,EleniceVianna,Pedro Thadeu Galvãoeng2013-03-06T00:00:00Zoai:scielo:S0102-86502013000300001Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-03-06T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
spellingShingle |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? Carraretto,Antônio Roberto Kidney Ischemia Reperfusion Hyperglycemia Propofol Isoflurane Rats |
title_short |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_full |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_fullStr |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_full_unstemmed |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_sort |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
author |
Carraretto,Antônio Roberto |
author_facet |
Carraretto,Antônio Roberto Vianna Filho,Pedro Thadeu Galvão Castiglia,Yara Marcondes Machado Golim,Marjorie de Assis Souza,Aparecida Vitória Gonçalves de Carvalho,Lídia Raquel de Deffune,Elenice Vianna,Pedro Thadeu Galvão |
author_role |
author |
author2 |
Vianna Filho,Pedro Thadeu Galvão Castiglia,Yara Marcondes Machado Golim,Marjorie de Assis Souza,Aparecida Vitória Gonçalves de Carvalho,Lídia Raquel de Deffune,Elenice Vianna,Pedro Thadeu Galvão |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Carraretto,Antônio Roberto Vianna Filho,Pedro Thadeu Galvão Castiglia,Yara Marcondes Machado Golim,Marjorie de Assis Souza,Aparecida Vitória Gonçalves de Carvalho,Lídia Raquel de Deffune,Elenice Vianna,Pedro Thadeu Galvão |
dc.subject.por.fl_str_mv |
Kidney Ischemia Reperfusion Hyperglycemia Propofol Isoflurane Rats |
topic |
Kidney Ischemia Reperfusion Hyperglycemia Propofol Isoflurane Rats |
description |
PURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000300001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502013000300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.28 n.3 2013 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126441702555648 |