Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000800011 |
Resumo: | PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered. |
id |
SBDPC-1_35cff2fadbb680cbb182ab573cd10a8f |
---|---|
oai_identifier_str |
oai:scielo:S0102-86502011000800011 |
network_acronym_str |
SBDPC-1 |
network_name_str |
Acta Cirúrgica Brasileira (Online) |
repository_id_str |
|
spelling |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogsIslets of Langerhans TransplantationDiabetes Mellitus, ExperimentalAnimal ExperimentationDogsPURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000800011Acta Cirúrgica Brasileira v.26 suppl.2 2011reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502011000800011info:eu-repo/semantics/openAccessWaisberg,JaquesNeff,Charles BenjaminWaisberg,Daniel ReisGermini,DemetriusGonçalves,José EduardoZanotto,ArnaldoSperanzini,Manlio Basilioeng2011-10-24T00:00:00Zoai:scielo:S0102-86502011000800011Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2011-10-24T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
spellingShingle |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs Waisberg,Jaques Islets of Langerhans Transplantation Diabetes Mellitus, Experimental Animal Experimentation Dogs |
title_short |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_full |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_fullStr |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_full_unstemmed |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
title_sort |
Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs |
author |
Waisberg,Jaques |
author_facet |
Waisberg,Jaques Neff,Charles Benjamin Waisberg,Daniel Reis Germini,Demetrius Gonçalves,José Eduardo Zanotto,Arnaldo Speranzini,Manlio Basilio |
author_role |
author |
author2 |
Neff,Charles Benjamin Waisberg,Daniel Reis Germini,Demetrius Gonçalves,José Eduardo Zanotto,Arnaldo Speranzini,Manlio Basilio |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Waisberg,Jaques Neff,Charles Benjamin Waisberg,Daniel Reis Germini,Demetrius Gonçalves,José Eduardo Zanotto,Arnaldo Speranzini,Manlio Basilio |
dc.subject.por.fl_str_mv |
Islets of Langerhans Transplantation Diabetes Mellitus, Experimental Animal Experimentation Dogs |
topic |
Islets of Langerhans Transplantation Diabetes Mellitus, Experimental Animal Experimentation Dogs |
description |
PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000800011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000800011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502011000800011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.26 suppl.2 2011 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126440826994688 |