Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy

• Autor(a) principal: Oliveira,Andrea Fernandes de
• Data de Publicação: 2010
• Outros Autores: Santos,Ivan Dunshee de Abranches Oliveira, Cartaxo,Sidney Bandeira, Bitar,Renata Andrade, Enokihara,Mílvia Maria Simões e Silva, Martinho,Herculano da Silva, Martin,Airton Abrahão, Ferreira,Lydia Masako
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000500009
• topic: Melanoma; Spectrum Analysis, Raman; Diagnosis; Biopsy

PURPOSE: To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis. METHODS: Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments of approximately 2 mm³ and positioned in the Raman spectrometer sample holder in order to obtain the spectra; a monochrome laser light Nd:YAG at 1064 nm was used to excite the inelastic effect. RESULTS: To differentiate the three histopathological groups according to their characteristics extracted from the spectra, data discriminative analysis was undertaken. Phenylalanine, DNA, and Amide-I spectral variables stood out in the differentiation of the three groups. The percentages of correctly classified groups based on Phenylalanine, DNA, and Amide-I spectral features was 93.1%. CONCLUSION: FT-Raman spectroscopy is capable of differentiating melanoma from its metastasis, as well as from normal skin.