Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000300007 |
Resumo: | PURPOSE: The pathophysiology of abnormalities associated with myenteric plexus lesions remains imperfectly understood. Such abnormalities have been correlated with subocclusive intestinal conditions in children with Hirschsprung's disease, cases of chronic constipation and, postoperatively, in cases of anorectal anomalies. This study evaluated abnormalities of the myenteric plexus in fetus from female rats that received ethylenethiourea. METHODS: Female rats were exposed to ethylenethiourea on the 11th day of pregnancy (experimental group) or to 0.9% physiological solution (control group). Abnormalities were only found in the experimental group. The digestive tract muscle layer was analyzed morphometrically and changes to the frequencies of nerve plexus cells and interstitial cells of Cajal were evaluated, using hematoxylin-eosin, S-100 protein, neuron-specific enolase and C-Kit, respectively. RESULTS: Muscle and skeletal abnormalities were observed in 100%, anorectal anomalies in 86%, absent tail in 71%, short tail in 29%, duodenal atresia in 5%, esophageal atresia in 5% and persistent omphalomesenteric duct in 5%. Histopathological analysis showed a thinner muscle layer associated with lower frequencies of ganglion cells and interstitial cells of Cajal, in all gastrointestinal tract. CONCLUSION: Severe nerve plexus abnormalities associated with muscle layer atrophy were observed throughout the gastrointestinal tract in newborn rats exposed to ethylenethiourea. |
id |
SBDPC-1_411462f3d74dc8958f2028fceb84cbc2 |
---|---|
oai_identifier_str |
oai:scielo:S0102-86502012000300007 |
network_acronym_str |
SBDPC-1 |
network_name_str |
Acta Cirúrgica Brasileira (Online) |
repository_id_str |
|
spelling |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethioureaEthylenethioureaMyenteric PlexusDigestive SystemHirschsprung DiseaseFetusRatsPURPOSE: The pathophysiology of abnormalities associated with myenteric plexus lesions remains imperfectly understood. Such abnormalities have been correlated with subocclusive intestinal conditions in children with Hirschsprung's disease, cases of chronic constipation and, postoperatively, in cases of anorectal anomalies. This study evaluated abnormalities of the myenteric plexus in fetus from female rats that received ethylenethiourea. METHODS: Female rats were exposed to ethylenethiourea on the 11th day of pregnancy (experimental group) or to 0.9% physiological solution (control group). Abnormalities were only found in the experimental group. The digestive tract muscle layer was analyzed morphometrically and changes to the frequencies of nerve plexus cells and interstitial cells of Cajal were evaluated, using hematoxylin-eosin, S-100 protein, neuron-specific enolase and C-Kit, respectively. RESULTS: Muscle and skeletal abnormalities were observed in 100%, anorectal anomalies in 86%, absent tail in 71%, short tail in 29%, duodenal atresia in 5%, esophageal atresia in 5% and persistent omphalomesenteric duct in 5%. Histopathological analysis showed a thinner muscle layer associated with lower frequencies of ganglion cells and interstitial cells of Cajal, in all gastrointestinal tract. CONCLUSION: Severe nerve plexus abnormalities associated with muscle layer atrophy were observed throughout the gastrointestinal tract in newborn rats exposed to ethylenethiourea.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000300007Acta Cirúrgica Brasileira v.27 n.3 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012000300007info:eu-repo/semantics/openAccessLemos,Sidney Pereira PintoMartins,José LuizLemos,Patrícia Veruska Ribeiro BarbosaSilva,Silvio Romero Gonçalves eSantos,Fernando Leandro dosSilva Júnior,Valdemiro Amaro daeng2012-03-27T00:00:00Zoai:scielo:S0102-86502012000300007Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-03-27T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
title |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
spellingShingle |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea Lemos,Sidney Pereira Pinto Ethylenethiourea Myenteric Plexus Digestive System Hirschsprung Disease Fetus Rats |
title_short |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
title_full |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
title_fullStr |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
title_full_unstemmed |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
title_sort |
Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea |
author |
Lemos,Sidney Pereira Pinto |
author_facet |
Lemos,Sidney Pereira Pinto Martins,José Luiz Lemos,Patrícia Veruska Ribeiro Barbosa Silva,Silvio Romero Gonçalves e Santos,Fernando Leandro dos Silva Júnior,Valdemiro Amaro da |
author_role |
author |
author2 |
Martins,José Luiz Lemos,Patrícia Veruska Ribeiro Barbosa Silva,Silvio Romero Gonçalves e Santos,Fernando Leandro dos Silva Júnior,Valdemiro Amaro da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lemos,Sidney Pereira Pinto Martins,José Luiz Lemos,Patrícia Veruska Ribeiro Barbosa Silva,Silvio Romero Gonçalves e Santos,Fernando Leandro dos Silva Júnior,Valdemiro Amaro da |
dc.subject.por.fl_str_mv |
Ethylenethiourea Myenteric Plexus Digestive System Hirschsprung Disease Fetus Rats |
topic |
Ethylenethiourea Myenteric Plexus Digestive System Hirschsprung Disease Fetus Rats |
description |
PURPOSE: The pathophysiology of abnormalities associated with myenteric plexus lesions remains imperfectly understood. Such abnormalities have been correlated with subocclusive intestinal conditions in children with Hirschsprung's disease, cases of chronic constipation and, postoperatively, in cases of anorectal anomalies. This study evaluated abnormalities of the myenteric plexus in fetus from female rats that received ethylenethiourea. METHODS: Female rats were exposed to ethylenethiourea on the 11th day of pregnancy (experimental group) or to 0.9% physiological solution (control group). Abnormalities were only found in the experimental group. The digestive tract muscle layer was analyzed morphometrically and changes to the frequencies of nerve plexus cells and interstitial cells of Cajal were evaluated, using hematoxylin-eosin, S-100 protein, neuron-specific enolase and C-Kit, respectively. RESULTS: Muscle and skeletal abnormalities were observed in 100%, anorectal anomalies in 86%, absent tail in 71%, short tail in 29%, duodenal atresia in 5%, esophageal atresia in 5% and persistent omphalomesenteric duct in 5%. Histopathological analysis showed a thinner muscle layer associated with lower frequencies of ganglion cells and interstitial cells of Cajal, in all gastrointestinal tract. CONCLUSION: Severe nerve plexus abnormalities associated with muscle layer atrophy were observed throughout the gastrointestinal tract in newborn rats exposed to ethylenethiourea. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502012000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.27 n.3 2012 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126440925560832 |