Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

• Autor(a) principal: Nascimento,Lízia Daniela e Silva
• Data de Publicação: 2019
• Outros Autores: Nicolau,Renata Amadei, Maia Filho,Antônio Luiz Martins, Santos,José Zilton Lima Verde, Fonseca,Khetyma Moreira, Ferreira,Danniel Cabral Leão, Sousa,Rayssilane Cardoso de, Viana,Vicente Galber Freitas, Carvalho,Luiz Fernando Meneses, Figueredo-Silva,José
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502019001100215
• topic: Achilles Tendon; Collagen; Rats

Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.