Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats

Detalhes bibliográficos
Autor(a) principal: Suartz,Caio Vinicius
Data de Publicação: 2014
Outros Autores: Gaiba,Silvana, França,Jerônimo Pereira de, Aloise,Antonio Carlos, Ferreira,Lydia Masako
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400003
Resumo: PURPOSE: To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS: Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS: The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro.The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSION:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.
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spelling Adipose-derived stem cells (ADSC) in the viability of random skin flap in ratsSurgical FlapsRatsAdult Stem CellsStem CellsAdipose TissuePURPOSE: To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS: Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS: The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro.The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSION:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400003Acta Cirúrgica Brasileira v.29 suppl.2 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014001400002info:eu-repo/semantics/openAccessSuartz,Caio ViniciusGaiba,SilvanaFrança,Jerônimo Pereira deAloise,Antonio CarlosFerreira,Lydia Masakoeng2015-10-08T00:00:00Zoai:scielo:S0102-86502014001400003Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
title Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
spellingShingle Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
Suartz,Caio Vinicius
Surgical Flaps
Rats
Adult Stem Cells
Stem Cells
Adipose Tissue
title_short Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
title_full Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
title_fullStr Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
title_full_unstemmed Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
title_sort Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
author Suartz,Caio Vinicius
author_facet Suartz,Caio Vinicius
Gaiba,Silvana
França,Jerônimo Pereira de
Aloise,Antonio Carlos
Ferreira,Lydia Masako
author_role author
author2 Gaiba,Silvana
França,Jerônimo Pereira de
Aloise,Antonio Carlos
Ferreira,Lydia Masako
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Suartz,Caio Vinicius
Gaiba,Silvana
França,Jerônimo Pereira de
Aloise,Antonio Carlos
Ferreira,Lydia Masako
dc.subject.por.fl_str_mv Surgical Flaps
Rats
Adult Stem Cells
Stem Cells
Adipose Tissue
topic Surgical Flaps
Rats
Adult Stem Cells
Stem Cells
Adipose Tissue
description PURPOSE: To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS: Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surgical procedure, the animals were randomized into two groups (n=15 each group), group control and group ADSC. In all groups the procedures were performed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day.RESULTS: The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro.The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05).CONCLUSION:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.The administration of adipose-derived stem cells was effective to increase the viability of the random skin flaps in rats.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014001400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 suppl.2 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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