Effect of L-Arginine and L-NAME treatments on polymorphonuclear leukocytes and mononuclear cells influx during tumor growth

• Autor(a) principal: Reis,Olívia Teixeira Gomes
• Data de Publicação: 2009
• Outros Autores: Raini,Juliana Crepaldi, Coradi,Silvana Torossian, Constantino,Dulce Helena Jardim
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000200006
• topic: Carcinoma, Ehrlich Tumor; Arginine; NG-Nitroarginine Methyl Ester; Leukocytes, Mononuclear; Nitric Oxide; Neutrophils; Mice

PURPOSE: Evaluate polymorphonuclear leukocytes (PMN's) and mononuclear cells (MN's) involvement in the Ehrlich´s solid tumor (ET) growth. METHODS: 90 Swiss mice were inoculated with 10(7) tumor cells (sc), distributed in three groups and treated once a day, via intraperitoneal (ip), with 0.1ml of diluent, L-Arginine (20mg/Kg) or L-NAME (20mg/Kg). After 7, 15 and 30 days of treatment, ten animals of each group were euthanized, the tumor mass was removed, processed and fixed for HE. Later, a morphometric analysis of the total area, parenchyma, necrosis, tumor stroma and PMN's leukocytes and MN's cells influx was performed. RESULTS: The L-Arginine treatment increased PMN's influx in the initial stage, whereas L-NAME reduced it. Our data suggests that NO effect on PMN's migration is dose-dependent. On the other hand, the MN´s cells influx was reduced by L-NAME treatment at all evaluated periods and at the same periods an increase in tumor growth was observed. CONCLUSION: At initial stages of tumor implantation, both PMN's leukocytes and MN's cells act together to control ET development.