Nuclear magnetic resonance spectroscopy in pancreatic disorders

Bibliographic Details
Main Author: Kaplan,Ofer
Publication Date: 1997
Format: Article
Language: eng
Source: Acta Cirúrgica Brasileira (Online)
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86501997000100002
Summary: Nuclear magnetic resonance spectroscopy (NMRS) is a powerful technique that enables continuous monitoring of biochemical processes in tissues and organs in a non-invasive manner. A model of isolated perfused rat pancreas, suitable for NMRS studies, was developed. Acute pancreatitis was induced by injections of either 0.5 ml 5% sodium taurocholate (TC) into the bile duets, or 1.0 ml 10% TC injections into the pancreatic parenchyma. Phosphorous (31P) NMRS of experimental pancreatitis were characterized by a transient signal at -0.18±0.04 ppm which was assigned as solubilized lecithin, and can be used as an indicator of the early phases of the discase. Depletion of the high energy phosphorous compounds, phosphocreatine and ATP, were also found during acute pancreatitis, and paralleled the extension of the pathological damage. The role of NMRS in pancreatic cancer diagnosis and its treatment were assessed in three models of pancreatic neoplasms. Perfused MIA PaCa-2 human pancreatic cancer cells, subcutancously implanted pancreatic tumors in hamsters, and pancreatic tumors induced in-situ in rats by direct appiication of the carcinogen 7,12-dimethyl benzanthracene, were studied by phosphorous (31P), sodium (23Na) and proton (¹H) NMRS. 31P spectra of pancreatic cancer were qualitatively similar to those of intact organs. However, 31P NMRS was found to be useful for monitoring the effects of treatment. Total (infra- and extracellular) sodium concentrations, measured in the solid tumors, were similar in both the normal pancreas and the pancreatic tumors (39-40 mmol/g wet weight). Proton spectra of perchloric acid extracts revealed several differences between tumors and control pancreases. The principal findings were elevated levels of the amino acid taurine, from I.17±O.39 mmol/g wet weight in healthy pancreases, to 2.79±0.71 mmol/g wet weight in pancreatic carcinoma in rats, and lactate levels which increased from 0.92±0.2 to 6.19±1.93 mmol/g wet weight, respectively. On the other hand, creatine and glutamate were higher in the normal pancreases. These studies demonstrated that NMRS is a useful technique for studying fundamental biochemical and metabolic events of acute pancreatitis and pancreatic cancer, and for the development of therapeutical modalities
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spelling Nuclear magnetic resonance spectroscopy in pancreatic disordersNuclear Magnetic ResonanceSpectroscopyPancreasNuclear magnetic resonance spectroscopy (NMRS) is a powerful technique that enables continuous monitoring of biochemical processes in tissues and organs in a non-invasive manner. A model of isolated perfused rat pancreas, suitable for NMRS studies, was developed. Acute pancreatitis was induced by injections of either 0.5 ml 5% sodium taurocholate (TC) into the bile duets, or 1.0 ml 10% TC injections into the pancreatic parenchyma. Phosphorous (31P) NMRS of experimental pancreatitis were characterized by a transient signal at -0.18±0.04 ppm which was assigned as solubilized lecithin, and can be used as an indicator of the early phases of the discase. Depletion of the high energy phosphorous compounds, phosphocreatine and ATP, were also found during acute pancreatitis, and paralleled the extension of the pathological damage. The role of NMRS in pancreatic cancer diagnosis and its treatment were assessed in three models of pancreatic neoplasms. Perfused MIA PaCa-2 human pancreatic cancer cells, subcutancously implanted pancreatic tumors in hamsters, and pancreatic tumors induced in-situ in rats by direct appiication of the carcinogen 7,12-dimethyl benzanthracene, were studied by phosphorous (31P), sodium (23Na) and proton (¹H) NMRS. 31P spectra of pancreatic cancer were qualitatively similar to those of intact organs. However, 31P NMRS was found to be useful for monitoring the effects of treatment. Total (infra- and extracellular) sodium concentrations, measured in the solid tumors, were similar in both the normal pancreas and the pancreatic tumors (39-40 mmol/g wet weight). Proton spectra of perchloric acid extracts revealed several differences between tumors and control pancreases. The principal findings were elevated levels of the amino acid taurine, from I.17±O.39 mmol/g wet weight in healthy pancreases, to 2.79±0.71 mmol/g wet weight in pancreatic carcinoma in rats, and lactate levels which increased from 0.92±0.2 to 6.19±1.93 mmol/g wet weight, respectively. On the other hand, creatine and glutamate were higher in the normal pancreases. These studies demonstrated that NMRS is a useful technique for studying fundamental biochemical and metabolic events of acute pancreatitis and pancreatic cancer, and for the development of therapeutical modalitiesSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia1997-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86501997000100002Acta Cirúrgica Brasileira v.12 n.1 1997reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86501997000100002info:eu-repo/semantics/openAccessKaplan,Ofereng2010-12-01T00:00:00Zoai:scielo:S0102-86501997000100002Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-12-01T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Nuclear magnetic resonance spectroscopy in pancreatic disorders
title Nuclear magnetic resonance spectroscopy in pancreatic disorders
spellingShingle Nuclear magnetic resonance spectroscopy in pancreatic disorders
Kaplan,Ofer
Nuclear Magnetic Resonance
Spectroscopy
Pancreas
title_short Nuclear magnetic resonance spectroscopy in pancreatic disorders
title_full Nuclear magnetic resonance spectroscopy in pancreatic disorders
title_fullStr Nuclear magnetic resonance spectroscopy in pancreatic disorders
title_full_unstemmed Nuclear magnetic resonance spectroscopy in pancreatic disorders
title_sort Nuclear magnetic resonance spectroscopy in pancreatic disorders
author Kaplan,Ofer
author_facet Kaplan,Ofer
author_role author
dc.contributor.author.fl_str_mv Kaplan,Ofer
dc.subject.por.fl_str_mv Nuclear Magnetic Resonance
Spectroscopy
Pancreas
topic Nuclear Magnetic Resonance
Spectroscopy
Pancreas
description Nuclear magnetic resonance spectroscopy (NMRS) is a powerful technique that enables continuous monitoring of biochemical processes in tissues and organs in a non-invasive manner. A model of isolated perfused rat pancreas, suitable for NMRS studies, was developed. Acute pancreatitis was induced by injections of either 0.5 ml 5% sodium taurocholate (TC) into the bile duets, or 1.0 ml 10% TC injections into the pancreatic parenchyma. Phosphorous (31P) NMRS of experimental pancreatitis were characterized by a transient signal at -0.18±0.04 ppm which was assigned as solubilized lecithin, and can be used as an indicator of the early phases of the discase. Depletion of the high energy phosphorous compounds, phosphocreatine and ATP, were also found during acute pancreatitis, and paralleled the extension of the pathological damage. The role of NMRS in pancreatic cancer diagnosis and its treatment were assessed in three models of pancreatic neoplasms. Perfused MIA PaCa-2 human pancreatic cancer cells, subcutancously implanted pancreatic tumors in hamsters, and pancreatic tumors induced in-situ in rats by direct appiication of the carcinogen 7,12-dimethyl benzanthracene, were studied by phosphorous (31P), sodium (23Na) and proton (¹H) NMRS. 31P spectra of pancreatic cancer were qualitatively similar to those of intact organs. However, 31P NMRS was found to be useful for monitoring the effects of treatment. Total (infra- and extracellular) sodium concentrations, measured in the solid tumors, were similar in both the normal pancreas and the pancreatic tumors (39-40 mmol/g wet weight). Proton spectra of perchloric acid extracts revealed several differences between tumors and control pancreases. The principal findings were elevated levels of the amino acid taurine, from I.17±O.39 mmol/g wet weight in healthy pancreases, to 2.79±0.71 mmol/g wet weight in pancreatic carcinoma in rats, and lactate levels which increased from 0.92±0.2 to 6.19±1.93 mmol/g wet weight, respectively. On the other hand, creatine and glutamate were higher in the normal pancreases. These studies demonstrated that NMRS is a useful technique for studying fundamental biochemical and metabolic events of acute pancreatitis and pancreatic cancer, and for the development of therapeutical modalities
publishDate 1997
dc.date.none.fl_str_mv 1997-03-01
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dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.12 n.1 1997
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instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
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instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
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