Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000500004 |
Resumo: | PURPOSE: To evaluate histopathologic alterations of the peritoneum exposed to heat shock. METHODS: Sixty rats were randomly distributed into 6 groups: Heat Shock (HS), High Temperature (HT), Body Temperature (BT), Temperature 0oC (TZ), Sham (SH) and Control (CG) with 10 animals each. The peritoneal cavity of animals from groups HS, HT, BT and TZ was irrigated with NaCl solution 0.9% at temperatures 50ºC, 0ºC, 50ºC, 37ºC and 0ºC, respectively. For animals from group SH, the procedures were simulated and those from group CG, laparotomy and biopsies were conducted. Twenty-four hours later, biopsies of the peritoneum for exams under light and electronic microscopy were performed. RESULTS: Edema was found in groups HS 80%, HT 60%, BT 30% TZ 70%, SH 40% and CG 30%. Vascular congestion was found in groups HS 20%, HT 30%, BT 10% and TZ 20%. Erythrocyte extravasation was found in groups HT 60% and SH 10%. Mesothelium destruction was found in 100% of specimens from groups HS, HT, BT, TZ, SH and CG 90%. Necrosis was found in groups HS 30%, HT 20% and BT 10%. The mean peritoneal thickness ranged from 42.26 µm (TZ) to 26.42 µm (CG). CONCLUSION: The heat shock caused no deaths, but promoted significant peritoneal edema without affecting the other histopathologic indicatives. |
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Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in ratsHeat-Shock ResponsePeritoneal LavagePeritonitisRatsPURPOSE: To evaluate histopathologic alterations of the peritoneum exposed to heat shock. METHODS: Sixty rats were randomly distributed into 6 groups: Heat Shock (HS), High Temperature (HT), Body Temperature (BT), Temperature 0oC (TZ), Sham (SH) and Control (CG) with 10 animals each. The peritoneal cavity of animals from groups HS, HT, BT and TZ was irrigated with NaCl solution 0.9% at temperatures 50ºC, 0ºC, 50ºC, 37ºC and 0ºC, respectively. For animals from group SH, the procedures were simulated and those from group CG, laparotomy and biopsies were conducted. Twenty-four hours later, biopsies of the peritoneum for exams under light and electronic microscopy were performed. RESULTS: Edema was found in groups HS 80%, HT 60%, BT 30% TZ 70%, SH 40% and CG 30%. Vascular congestion was found in groups HS 20%, HT 30%, BT 10% and TZ 20%. Erythrocyte extravasation was found in groups HT 60% and SH 10%. Mesothelium destruction was found in 100% of specimens from groups HS, HT, BT, TZ, SH and CG 90%. Necrosis was found in groups HS 30%, HT 20% and BT 10%. The mean peritoneal thickness ranged from 42.26 µm (TZ) to 26.42 µm (CG). CONCLUSION: The heat shock caused no deaths, but promoted significant peritoneal edema without affecting the other histopathologic indicatives.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000500004Acta Cirúrgica Brasileira v.22 n.5 2007reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502007000500004info:eu-repo/semantics/openAccessLopes,João VieiraOliveira,Paulo Gonçalves deSousa,João Batista deBáo,Sonia NairTakano,Gustavo Henrique SoaresLeal,Isabel Irene Ramaeng2007-09-27T00:00:00Zoai:scielo:S0102-86502007000500004Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2007-09-27T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
title |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
spellingShingle |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats Lopes,João Vieira Heat-Shock Response Peritoneal Lavage Peritonitis Rats |
title_short |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
title_full |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
title_fullStr |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
title_full_unstemmed |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
title_sort |
Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats |
author |
Lopes,João Vieira |
author_facet |
Lopes,João Vieira Oliveira,Paulo Gonçalves de Sousa,João Batista de Báo,Sonia Nair Takano,Gustavo Henrique Soares Leal,Isabel Irene Rama |
author_role |
author |
author2 |
Oliveira,Paulo Gonçalves de Sousa,João Batista de Báo,Sonia Nair Takano,Gustavo Henrique Soares Leal,Isabel Irene Rama |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lopes,João Vieira Oliveira,Paulo Gonçalves de Sousa,João Batista de Báo,Sonia Nair Takano,Gustavo Henrique Soares Leal,Isabel Irene Rama |
dc.subject.por.fl_str_mv |
Heat-Shock Response Peritoneal Lavage Peritonitis Rats |
topic |
Heat-Shock Response Peritoneal Lavage Peritonitis Rats |
description |
PURPOSE: To evaluate histopathologic alterations of the peritoneum exposed to heat shock. METHODS: Sixty rats were randomly distributed into 6 groups: Heat Shock (HS), High Temperature (HT), Body Temperature (BT), Temperature 0oC (TZ), Sham (SH) and Control (CG) with 10 animals each. The peritoneal cavity of animals from groups HS, HT, BT and TZ was irrigated with NaCl solution 0.9% at temperatures 50ºC, 0ºC, 50ºC, 37ºC and 0ºC, respectively. For animals from group SH, the procedures were simulated and those from group CG, laparotomy and biopsies were conducted. Twenty-four hours later, biopsies of the peritoneum for exams under light and electronic microscopy were performed. RESULTS: Edema was found in groups HS 80%, HT 60%, BT 30% TZ 70%, SH 40% and CG 30%. Vascular congestion was found in groups HS 20%, HT 30%, BT 10% and TZ 20%. Erythrocyte extravasation was found in groups HT 60% and SH 10%. Mesothelium destruction was found in 100% of specimens from groups HS, HT, BT, TZ, SH and CG 90%. Necrosis was found in groups HS 30%, HT 20% and BT 10%. The mean peritoneal thickness ranged from 42.26 µm (TZ) to 26.42 µm (CG). CONCLUSION: The heat shock caused no deaths, but promoted significant peritoneal edema without affecting the other histopathologic indicatives. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000500004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502007000500004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.22 n.5 2007 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126438684753920 |