Evaluation of lipoic acid topical application on rats skin wound healing

Detalhes bibliográficos
Autor(a) principal: Külkamp-Guerreiro,Irene Clemes
Data de Publicação: 2013
Outros Autores: Souza,Marielly Nunes, Bianchin,Mariana Domingues, Isoppo,Mateus, Freitas,Joana Sachetti, Alves,João Alex, Piovezan,Anna Paula, Pohlmann,Adriana Raffin, Guterres,Sílvia Stanisçuaski
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001000004
Resumo: PURPOSE: To evaluate the effects of lipoic acid (thioctic acid) topical application on wound healing on rats skin, and the consequences of lipoic acid nanoencapsulation on this process. METHODS: The model used was the healing activity on wounds induced by surgical incision on rats skin (n = 44). The parameters analyzed (11 days) were wound healing rate and histology (vascular proliferation, polymorphonuclear or mononuclear cells, and collagen synthesis or reepithelialization), after application of free lipoic acid or lipoic acid- loaded nanocapsules. The antioxidant activity of these formulations was evaluated by lipid peroxidation test. RESULTS: It was demonstrated for the first time that the topical application of lipoic acid improves wound healing. On the seventh day after surgery, the animals treated with lipoic acid showed increased healing rate (60.7 ± 8.4%) compared to the negative control group (43.0 ± 17.4%), as so improvement of histological parameters. The nanoencapsulation reverted the pro-oxidant activity presented in vitro by lipoic acid, whereas diminished wound repair. CONCLUSIONS: The topical application of lipoic acid produced an increase in the skin wound healing, which may be related to its pro-oxidant activity. On the other hand, the nanoencapsulation of the lipoic acid reversed the pro-oxidant activity, although presented minor healing activity.
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spelling Evaluation of lipoic acid topical application on rats skin wound healingThioctic AcidWound HealingNanocapsulesPURPOSE: To evaluate the effects of lipoic acid (thioctic acid) topical application on wound healing on rats skin, and the consequences of lipoic acid nanoencapsulation on this process. METHODS: The model used was the healing activity on wounds induced by surgical incision on rats skin (n = 44). The parameters analyzed (11 days) were wound healing rate and histology (vascular proliferation, polymorphonuclear or mononuclear cells, and collagen synthesis or reepithelialization), after application of free lipoic acid or lipoic acid- loaded nanocapsules. The antioxidant activity of these formulations was evaluated by lipid peroxidation test. RESULTS: It was demonstrated for the first time that the topical application of lipoic acid improves wound healing. On the seventh day after surgery, the animals treated with lipoic acid showed increased healing rate (60.7 ± 8.4%) compared to the negative control group (43.0 ± 17.4%), as so improvement of histological parameters. The nanoencapsulation reverted the pro-oxidant activity presented in vitro by lipoic acid, whereas diminished wound repair. CONCLUSIONS: The topical application of lipoic acid produced an increase in the skin wound healing, which may be related to its pro-oxidant activity. On the other hand, the nanoencapsulation of the lipoic acid reversed the pro-oxidant activity, although presented minor healing activity.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001000004Acta Cirúrgica Brasileira v.28 n.10 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013001000004info:eu-repo/semantics/openAccessKülkamp-Guerreiro,Irene ClemesSouza,Marielly NunesBianchin,Mariana DominguesIsoppo,MateusFreitas,Joana SachettiAlves,João AlexPiovezan,Anna PaulaPohlmann,Adriana RaffinGuterres,Sílvia Stanisçuaskieng2013-10-07T00:00:00Zoai:scielo:S0102-86502013001000004Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-10-07T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Evaluation of lipoic acid topical application on rats skin wound healing
title Evaluation of lipoic acid topical application on rats skin wound healing
spellingShingle Evaluation of lipoic acid topical application on rats skin wound healing
Külkamp-Guerreiro,Irene Clemes
Thioctic Acid
Wound Healing
Nanocapsules
title_short Evaluation of lipoic acid topical application on rats skin wound healing
title_full Evaluation of lipoic acid topical application on rats skin wound healing
title_fullStr Evaluation of lipoic acid topical application on rats skin wound healing
title_full_unstemmed Evaluation of lipoic acid topical application on rats skin wound healing
title_sort Evaluation of lipoic acid topical application on rats skin wound healing
author Külkamp-Guerreiro,Irene Clemes
author_facet Külkamp-Guerreiro,Irene Clemes
Souza,Marielly Nunes
Bianchin,Mariana Domingues
Isoppo,Mateus
Freitas,Joana Sachetti
Alves,João Alex
Piovezan,Anna Paula
Pohlmann,Adriana Raffin
Guterres,Sílvia Stanisçuaski
author_role author
author2 Souza,Marielly Nunes
Bianchin,Mariana Domingues
Isoppo,Mateus
Freitas,Joana Sachetti
Alves,João Alex
Piovezan,Anna Paula
Pohlmann,Adriana Raffin
Guterres,Sílvia Stanisçuaski
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Külkamp-Guerreiro,Irene Clemes
Souza,Marielly Nunes
Bianchin,Mariana Domingues
Isoppo,Mateus
Freitas,Joana Sachetti
Alves,João Alex
Piovezan,Anna Paula
Pohlmann,Adriana Raffin
Guterres,Sílvia Stanisçuaski
dc.subject.por.fl_str_mv Thioctic Acid
Wound Healing
Nanocapsules
topic Thioctic Acid
Wound Healing
Nanocapsules
description PURPOSE: To evaluate the effects of lipoic acid (thioctic acid) topical application on wound healing on rats skin, and the consequences of lipoic acid nanoencapsulation on this process. METHODS: The model used was the healing activity on wounds induced by surgical incision on rats skin (n = 44). The parameters analyzed (11 days) were wound healing rate and histology (vascular proliferation, polymorphonuclear or mononuclear cells, and collagen synthesis or reepithelialization), after application of free lipoic acid or lipoic acid- loaded nanocapsules. The antioxidant activity of these formulations was evaluated by lipid peroxidation test. RESULTS: It was demonstrated for the first time that the topical application of lipoic acid improves wound healing. On the seventh day after surgery, the animals treated with lipoic acid showed increased healing rate (60.7 ± 8.4%) compared to the negative control group (43.0 ± 17.4%), as so improvement of histological parameters. The nanoencapsulation reverted the pro-oxidant activity presented in vitro by lipoic acid, whereas diminished wound repair. CONCLUSIONS: The topical application of lipoic acid produced an increase in the skin wound healing, which may be related to its pro-oxidant activity. On the other hand, the nanoencapsulation of the lipoic acid reversed the pro-oxidant activity, although presented minor healing activity.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001000004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001000004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502013001000004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.28 n.10 2013
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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