Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes

Detalhes bibliográficos
Autor(a) principal: Hu,Zhao
Data de Publicação: 2018
Outros Autores: Cai,Hong-Yan, Luo,Yun-Yan, Xiao,Jian-Ming, Li,Lin, Guo,Tao
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300223
Resumo: Abstract Purpose: To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2). Methods: Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay. Results: Cell viabilities in HR model groups were significantly lower than those of group A (P<0.05). LC3 II/LC3 I levels in groups B to D were significantly higher than those of group A (P<0.05), and group D showed the highest LC3 II/LC3 I levels. Cell viabilities in groups B to D were significantly lower than those of group A (P<0.05), with group D showing the lowest cell viabilities (P<0.05). Conclusions: Hypoxia can induce autophagy in rat cardiomyocytes, which can be further activated by reoxygenation; most notable after 12 h. Hypoxia-induced cell injury can be aggravated by reoxygenation. The lowest cell viability was observed at 12 h after reoxygenation; however, cell viability can be recovered after 24 h.
id SBDPC-1_791fbf53b80233205e918c67af726700
oai_identifier_str oai:scielo:S0102-86502018000300223
network_acronym_str SBDPC-1
network_name_str Acta Cirúrgica Brasileira (Online)
repository_id_str
spelling Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytesAutophagyMyocytes, CardiacHypoxiaRats.Abstract Purpose: To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2). Methods: Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay. Results: Cell viabilities in HR model groups were significantly lower than those of group A (P<0.05). LC3 II/LC3 I levels in groups B to D were significantly higher than those of group A (P<0.05), and group D showed the highest LC3 II/LC3 I levels. Cell viabilities in groups B to D were significantly lower than those of group A (P<0.05), with group D showing the lowest cell viabilities (P<0.05). Conclusions: Hypoxia can induce autophagy in rat cardiomyocytes, which can be further activated by reoxygenation; most notable after 12 h. Hypoxia-induced cell injury can be aggravated by reoxygenation. The lowest cell viability was observed at 12 h after reoxygenation; however, cell viability can be recovered after 24 h.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300223Acta Cirúrgica Brasileira v.33 n.3 2018reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020180030000004info:eu-repo/semantics/openAccessHu,ZhaoCai,Hong-YanLuo,Yun-YanXiao,Jian-MingLi,LinGuo,Taoeng2018-04-09T00:00:00Zoai:scielo:S0102-86502018000300223Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2018-04-09T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
title Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
spellingShingle Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
Hu,Zhao
Autophagy
Myocytes, Cardiac
Hypoxia
Rats.
title_short Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
title_full Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
title_fullStr Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
title_full_unstemmed Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
title_sort Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
author Hu,Zhao
author_facet Hu,Zhao
Cai,Hong-Yan
Luo,Yun-Yan
Xiao,Jian-Ming
Li,Lin
Guo,Tao
author_role author
author2 Cai,Hong-Yan
Luo,Yun-Yan
Xiao,Jian-Ming
Li,Lin
Guo,Tao
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Hu,Zhao
Cai,Hong-Yan
Luo,Yun-Yan
Xiao,Jian-Ming
Li,Lin
Guo,Tao
dc.subject.por.fl_str_mv Autophagy
Myocytes, Cardiac
Hypoxia
Rats.
topic Autophagy
Myocytes, Cardiac
Hypoxia
Rats.
description Abstract Purpose: To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2). Methods: Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay. Results: Cell viabilities in HR model groups were significantly lower than those of group A (P<0.05). LC3 II/LC3 I levels in groups B to D were significantly higher than those of group A (P<0.05), and group D showed the highest LC3 II/LC3 I levels. Cell viabilities in groups B to D were significantly lower than those of group A (P<0.05), with group D showing the lowest cell viabilities (P<0.05). Conclusions: Hypoxia can induce autophagy in rat cardiomyocytes, which can be further activated by reoxygenation; most notable after 12 h. Hypoxia-induced cell injury can be aggravated by reoxygenation. The lowest cell viability was observed at 12 h after reoxygenation; however, cell viability can be recovered after 24 h.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300223
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000300223
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020180030000004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.33 n.3 2018
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
_version_ 1752126444605014016