Evidence of presynaptic and postsynaptic action of local anesthetics in rats

Detalhes bibliográficos
Autor(a) principal: Braga,Angélica de Fátima de Assunção
Data de Publicação: 2013
Outros Autores: Carvalho,Vanessa Henriques, Braga,Franklin Sarmento da Silva, Potério,Gloria Maria Braga, Santos,Filipe Nadir Caparica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001100005
Resumo: PURPOSE: To assess the probable actions of ropivacaine, 50% enantiomeric excess bupivacaine mixture (S75-R25) and levobupivacaine on neuromuscular transmission in vitro. METHODS: Thirty rats were distributed into groups (n=5) according to the drug used: ropivacaine, bupivacaine (S75-R25) and levobupivacaine. The concentration used for the three local anesthetics (LA) was 5 µg.mL.-1The following parameters were evaluated: 1) LA effects on membrane potential (MP) and miniature end plate potential (MEPP). A chick biventer cervicis preparation was also used to evaluate LA effects on the contracture response to acetylcholine. RESULTS: LA did not alter MP values and decreased the frequency and amplitude of MEPP. In a chick biventer cervicis preparation, bupivacaine (S75-R25) and levobupivacaine decreased the contracture response to acetylcholine with statistical significance, in comparison to ropivacaine. CONCLUSIONS: In the concentrations used, levobupivacaine and bupivacaine (S75-R25) exhibited presynaptic and postsynaptic actions evidenced by alterations in miniature end plate potentials and contracture response to acetylcholine. Ropivacaine only had a presynaptic action.
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spelling Evidence of presynaptic and postsynaptic action of local anesthetics in ratsAnesthetics, LocalBupivacaineRatsPURPOSE: To assess the probable actions of ropivacaine, 50% enantiomeric excess bupivacaine mixture (S75-R25) and levobupivacaine on neuromuscular transmission in vitro. METHODS: Thirty rats were distributed into groups (n=5) according to the drug used: ropivacaine, bupivacaine (S75-R25) and levobupivacaine. The concentration used for the three local anesthetics (LA) was 5 µg.mL.-1The following parameters were evaluated: 1) LA effects on membrane potential (MP) and miniature end plate potential (MEPP). A chick biventer cervicis preparation was also used to evaluate LA effects on the contracture response to acetylcholine. RESULTS: LA did not alter MP values and decreased the frequency and amplitude of MEPP. In a chick biventer cervicis preparation, bupivacaine (S75-R25) and levobupivacaine decreased the contracture response to acetylcholine with statistical significance, in comparison to ropivacaine. CONCLUSIONS: In the concentrations used, levobupivacaine and bupivacaine (S75-R25) exhibited presynaptic and postsynaptic actions evidenced by alterations in miniature end plate potentials and contracture response to acetylcholine. Ropivacaine only had a presynaptic action.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2013-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001100005Acta Cirúrgica Brasileira v.28 n.11 2013reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502013001100005info:eu-repo/semantics/openAccessBraga,Angélica de Fátima de AssunçãoCarvalho,Vanessa HenriquesBraga,Franklin Sarmento da SilvaPotério,Gloria Maria BragaSantos,Filipe Nadir Caparicaeng2013-12-05T00:00:00Zoai:scielo:S0102-86502013001100005Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2013-12-05T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Evidence of presynaptic and postsynaptic action of local anesthetics in rats
title Evidence of presynaptic and postsynaptic action of local anesthetics in rats
spellingShingle Evidence of presynaptic and postsynaptic action of local anesthetics in rats
Braga,Angélica de Fátima de Assunção
Anesthetics, Local
Bupivacaine
Rats
title_short Evidence of presynaptic and postsynaptic action of local anesthetics in rats
title_full Evidence of presynaptic and postsynaptic action of local anesthetics in rats
title_fullStr Evidence of presynaptic and postsynaptic action of local anesthetics in rats
title_full_unstemmed Evidence of presynaptic and postsynaptic action of local anesthetics in rats
title_sort Evidence of presynaptic and postsynaptic action of local anesthetics in rats
author Braga,Angélica de Fátima de Assunção
author_facet Braga,Angélica de Fátima de Assunção
Carvalho,Vanessa Henriques
Braga,Franklin Sarmento da Silva
Potério,Gloria Maria Braga
Santos,Filipe Nadir Caparica
author_role author
author2 Carvalho,Vanessa Henriques
Braga,Franklin Sarmento da Silva
Potério,Gloria Maria Braga
Santos,Filipe Nadir Caparica
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Braga,Angélica de Fátima de Assunção
Carvalho,Vanessa Henriques
Braga,Franklin Sarmento da Silva
Potério,Gloria Maria Braga
Santos,Filipe Nadir Caparica
dc.subject.por.fl_str_mv Anesthetics, Local
Bupivacaine
Rats
topic Anesthetics, Local
Bupivacaine
Rats
description PURPOSE: To assess the probable actions of ropivacaine, 50% enantiomeric excess bupivacaine mixture (S75-R25) and levobupivacaine on neuromuscular transmission in vitro. METHODS: Thirty rats were distributed into groups (n=5) according to the drug used: ropivacaine, bupivacaine (S75-R25) and levobupivacaine. The concentration used for the three local anesthetics (LA) was 5 µg.mL.-1The following parameters were evaluated: 1) LA effects on membrane potential (MP) and miniature end plate potential (MEPP). A chick biventer cervicis preparation was also used to evaluate LA effects on the contracture response to acetylcholine. RESULTS: LA did not alter MP values and decreased the frequency and amplitude of MEPP. In a chick biventer cervicis preparation, bupivacaine (S75-R25) and levobupivacaine decreased the contracture response to acetylcholine with statistical significance, in comparison to ropivacaine. CONCLUSIONS: In the concentrations used, levobupivacaine and bupivacaine (S75-R25) exhibited presynaptic and postsynaptic actions evidenced by alterations in miniature end plate potentials and contracture response to acetylcholine. Ropivacaine only had a presynaptic action.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001100005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013001100005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502013001100005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.28 n.11 2013
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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