AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001200005 |
Resumo: | PURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation. |
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Acta Cirúrgica Brasileira (Online) |
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AZT on telomerase activity and cell proliferation in HS 839.T melanoma cellsTelomeraseMelanomaZidovudineCell ProliferationIn VitroPURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001200005Acta Cirúrgica Brasileira v.27 n.12 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012001200005info:eu-repo/semantics/openAccessSouza Sobrinho,Celestino Prospero deGragnani,AlfredoSantos,Ivan Dunshee Abranches OliveiraOliveira,Andrea FernandesLipay,Monica Vanucci NunesFerreira,Lydia Masakoeng2012-11-29T00:00:00Zoai:scielo:S0102-86502012001200005Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-11-29T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
title |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
spellingShingle |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells Souza Sobrinho,Celestino Prospero de Telomerase Melanoma Zidovudine Cell Proliferation In Vitro |
title_short |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
title_full |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
title_fullStr |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
title_full_unstemmed |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
title_sort |
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells |
author |
Souza Sobrinho,Celestino Prospero de |
author_facet |
Souza Sobrinho,Celestino Prospero de Gragnani,Alfredo Santos,Ivan Dunshee Abranches Oliveira Oliveira,Andrea Fernandes Lipay,Monica Vanucci Nunes Ferreira,Lydia Masako |
author_role |
author |
author2 |
Gragnani,Alfredo Santos,Ivan Dunshee Abranches Oliveira Oliveira,Andrea Fernandes Lipay,Monica Vanucci Nunes Ferreira,Lydia Masako |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza Sobrinho,Celestino Prospero de Gragnani,Alfredo Santos,Ivan Dunshee Abranches Oliveira Oliveira,Andrea Fernandes Lipay,Monica Vanucci Nunes Ferreira,Lydia Masako |
dc.subject.por.fl_str_mv |
Telomerase Melanoma Zidovudine Cell Proliferation In Vitro |
topic |
Telomerase Melanoma Zidovudine Cell Proliferation In Vitro |
description |
PURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502012001200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.27 n.12 2012 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126441387982848 |