Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046 |
Resumo: | Abstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment. |
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Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in ratsMagnetic Field TherapyMagnetite NanoparticlesMethicillin-Resistant Staphylococcus aureusRatsAbstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046Acta Cirúrgica Brasileira v.32 n.1 2017reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020170106info:eu-repo/semantics/openAccessCarvalho,Juliana Fernandes deAzevedo,Ítalo Medeiros deRocha,Keyla Borges FerreiraMedeiros,Aldo CunhaCarriço,Artur da Silvaeng2017-02-14T00:00:00Zoai:scielo:S0102-86502017000100046Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2017-02-14T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
spellingShingle |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats Carvalho,Juliana Fernandes de Magnetic Field Therapy Magnetite Nanoparticles Methicillin-Resistant Staphylococcus aureus Rats |
title_short |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_full |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_fullStr |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_full_unstemmed |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_sort |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
author |
Carvalho,Juliana Fernandes de |
author_facet |
Carvalho,Juliana Fernandes de Azevedo,Ítalo Medeiros de Rocha,Keyla Borges Ferreira Medeiros,Aldo Cunha Carriço,Artur da Silva |
author_role |
author |
author2 |
Azevedo,Ítalo Medeiros de Rocha,Keyla Borges Ferreira Medeiros,Aldo Cunha Carriço,Artur da Silva |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho,Juliana Fernandes de Azevedo,Ítalo Medeiros de Rocha,Keyla Borges Ferreira Medeiros,Aldo Cunha Carriço,Artur da Silva |
dc.subject.por.fl_str_mv |
Magnetic Field Therapy Magnetite Nanoparticles Methicillin-Resistant Staphylococcus aureus Rats |
topic |
Magnetic Field Therapy Magnetite Nanoparticles Methicillin-Resistant Staphylococcus aureus Rats |
description |
Abstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0102-865020170106 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.32 n.1 2017 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126444103794688 |