Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502004000400003 |
Resumo: | PURPOSE: Ischemia and reperfusion of the small intestine disrupts gut barrier, causes bacterial translocation and activates inflammatory responses. An experimental study was planned to evaluate if 99mTc labelled Escherichia coli translocates to mesenteric lymph nodes, liver, spleen, lung and serum of rats submitted to mesenteric ischemia/reperfusion. Additionally, it was observed if the time of reperfusion influences the level of translocation. METHODS: Forty male Wistar rats underwent 45 minutes of gut ischemia by occlusion of the superior mesenteric artery. The translocation of labelled bacteria to different organs and portal serum was determined in rats reperfused for 30 minutes, 24 hours, sham(S) and controls(C), using radioactivity count and colony forming units/g (CFU). RESULTS: All the organs from rats observed for 24 hours after reperfusion had higher levels of radioactivity and positive cultures (CFU) than did the organs of rats reperfused for 30 minutes, C and S, except in the spleen (p<0,01). CONCLUSION: The results of this study indicated that intestinal ischemia/reperfusion led to bacterial translocation, mostly after 24 hours of reperfusion. |
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Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusionBacterial translocationIschemiaReperfusionIntestine99mTechnecium labelled bacteriaPURPOSE: Ischemia and reperfusion of the small intestine disrupts gut barrier, causes bacterial translocation and activates inflammatory responses. An experimental study was planned to evaluate if 99mTc labelled Escherichia coli translocates to mesenteric lymph nodes, liver, spleen, lung and serum of rats submitted to mesenteric ischemia/reperfusion. Additionally, it was observed if the time of reperfusion influences the level of translocation. METHODS: Forty male Wistar rats underwent 45 minutes of gut ischemia by occlusion of the superior mesenteric artery. The translocation of labelled bacteria to different organs and portal serum was determined in rats reperfused for 30 minutes, 24 hours, sham(S) and controls(C), using radioactivity count and colony forming units/g (CFU). RESULTS: All the organs from rats observed for 24 hours after reperfusion had higher levels of radioactivity and positive cultures (CFU) than did the organs of rats reperfused for 30 minutes, C and S, except in the spleen (p<0,01). CONCLUSION: The results of this study indicated that intestinal ischemia/reperfusion led to bacterial translocation, mostly after 24 hours of reperfusion.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502004000400003Acta Cirúrgica Brasileira v.19 n.4 2004reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502004000400003info:eu-repo/semantics/openAccessJoão,Samir AssiAlencar,Suelene Suassuna Silvestre deMedeiros,Aldo da CunhaDiniz,Simone Otília FernandesCardoso,Valbert NascimentoBrandt,Carlos Teixeiraeng2004-08-12T00:00:00Zoai:scielo:S0102-86502004000400003Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2004-08-12T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
title |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
spellingShingle |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion João,Samir Assi Bacterial translocation Ischemia Reperfusion Intestine 99mTechnecium labelled bacteria |
title_short |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
title_full |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
title_fullStr |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
title_full_unstemmed |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
title_sort |
Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion |
author |
João,Samir Assi |
author_facet |
João,Samir Assi Alencar,Suelene Suassuna Silvestre de Medeiros,Aldo da Cunha Diniz,Simone Otília Fernandes Cardoso,Valbert Nascimento Brandt,Carlos Teixeira |
author_role |
author |
author2 |
Alencar,Suelene Suassuna Silvestre de Medeiros,Aldo da Cunha Diniz,Simone Otília Fernandes Cardoso,Valbert Nascimento Brandt,Carlos Teixeira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
João,Samir Assi Alencar,Suelene Suassuna Silvestre de Medeiros,Aldo da Cunha Diniz,Simone Otília Fernandes Cardoso,Valbert Nascimento Brandt,Carlos Teixeira |
dc.subject.por.fl_str_mv |
Bacterial translocation Ischemia Reperfusion Intestine 99mTechnecium labelled bacteria |
topic |
Bacterial translocation Ischemia Reperfusion Intestine 99mTechnecium labelled bacteria |
description |
PURPOSE: Ischemia and reperfusion of the small intestine disrupts gut barrier, causes bacterial translocation and activates inflammatory responses. An experimental study was planned to evaluate if 99mTc labelled Escherichia coli translocates to mesenteric lymph nodes, liver, spleen, lung and serum of rats submitted to mesenteric ischemia/reperfusion. Additionally, it was observed if the time of reperfusion influences the level of translocation. METHODS: Forty male Wistar rats underwent 45 minutes of gut ischemia by occlusion of the superior mesenteric artery. The translocation of labelled bacteria to different organs and portal serum was determined in rats reperfused for 30 minutes, 24 hours, sham(S) and controls(C), using radioactivity count and colony forming units/g (CFU). RESULTS: All the organs from rats observed for 24 hours after reperfusion had higher levels of radioactivity and positive cultures (CFU) than did the organs of rats reperfused for 30 minutes, C and S, except in the spleen (p<0,01). CONCLUSION: The results of this study indicated that intestinal ischemia/reperfusion led to bacterial translocation, mostly after 24 hours of reperfusion. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502004000400003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502004000400003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502004000400003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.19 n.4 2004 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126437278613504 |