Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500008 |
Resumo: | PURPOSE: To study the effect of protein malnutrition on the intestinal wall of rats by evaluating alterations in the rupture force and dosing tissue collagen in the ileum and distal colon. METHODS: One hundred and twenty rats, that had an average weight of 100g, were used. They received water and a standard diet with 20% protein during 7 days for adaptation to the diet itself and to environmental conditions. After that period, the animals were randomly distributed in two groups of 60 rats each: Group 1 - the animals received a control diet with 20% casein for 21 days; Group 2 - hypoprotein diet with 2% casein for 21 days. After the adaptation period, 12 animals of each group were sacrificed at 5 moments: the beginning of experimental period (M0), 4° day (M1), 7° day (M2), 14° day (M3) and 21° day (M4). The diet to the other rats was maintained until the last sacrifice. The following variables were evaluated: body weight, blood albumin rate, tissue's hydroxyproline, hydroxyproline/total protein ratio and rupture strength in the intestinal wall of the ileum and the distal colon. RESULTS: It was observed that the rupture strength in the ileum segment and distal colon was lower in malnourished animals (Group 2); the loss of mechanical resistance was higher in the distal colon segment than in the ileum probably due to the smaller concentration of tissue collagen in the distal colon. CONCLUSION: Protein malnutrition induces the loss of mechanical resistance of the ileum and distal colon and may be associated with a smaller percentage of collagenous tissue formation in the intestinal wall. |
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Acta Cirúrgica Brasileira (Online) |
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Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagenProtein MalnutritionAnastomosis, SurgicalTensile StrengthCollagenRatsPURPOSE: To study the effect of protein malnutrition on the intestinal wall of rats by evaluating alterations in the rupture force and dosing tissue collagen in the ileum and distal colon. METHODS: One hundred and twenty rats, that had an average weight of 100g, were used. They received water and a standard diet with 20% protein during 7 days for adaptation to the diet itself and to environmental conditions. After that period, the animals were randomly distributed in two groups of 60 rats each: Group 1 - the animals received a control diet with 20% casein for 21 days; Group 2 - hypoprotein diet with 2% casein for 21 days. After the adaptation period, 12 animals of each group were sacrificed at 5 moments: the beginning of experimental period (M0), 4° day (M1), 7° day (M2), 14° day (M3) and 21° day (M4). The diet to the other rats was maintained until the last sacrifice. The following variables were evaluated: body weight, blood albumin rate, tissue's hydroxyproline, hydroxyproline/total protein ratio and rupture strength in the intestinal wall of the ileum and the distal colon. RESULTS: It was observed that the rupture strength in the ileum segment and distal colon was lower in malnourished animals (Group 2); the loss of mechanical resistance was higher in the distal colon segment than in the ileum probably due to the smaller concentration of tissue collagen in the distal colon. CONCLUSION: Protein malnutrition induces the loss of mechanical resistance of the ileum and distal colon and may be associated with a smaller percentage of collagenous tissue formation in the intestinal wall.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500008Acta Cirúrgica Brasileira v.23 n.5 2008reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502008000500008info:eu-repo/semantics/openAccessNakajima,VictorKobayasi,ShoitiNaresse,Luís EduardoLeite,Celso Vieira de SouzaCuri,Paulo RobertoMontovani,Jair Cortezeng2008-09-03T00:00:00Zoai:scielo:S0102-86502008000500008Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2008-09-03T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
title |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
spellingShingle |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen Nakajima,Victor Protein Malnutrition Anastomosis, Surgical Tensile Strength Collagen Rats |
title_short |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
title_full |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
title_fullStr |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
title_full_unstemmed |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
title_sort |
Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen |
author |
Nakajima,Victor |
author_facet |
Nakajima,Victor Kobayasi,Shoiti Naresse,Luís Eduardo Leite,Celso Vieira de Souza Curi,Paulo Roberto Montovani,Jair Cortez |
author_role |
author |
author2 |
Kobayasi,Shoiti Naresse,Luís Eduardo Leite,Celso Vieira de Souza Curi,Paulo Roberto Montovani,Jair Cortez |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Nakajima,Victor Kobayasi,Shoiti Naresse,Luís Eduardo Leite,Celso Vieira de Souza Curi,Paulo Roberto Montovani,Jair Cortez |
dc.subject.por.fl_str_mv |
Protein Malnutrition Anastomosis, Surgical Tensile Strength Collagen Rats |
topic |
Protein Malnutrition Anastomosis, Surgical Tensile Strength Collagen Rats |
description |
PURPOSE: To study the effect of protein malnutrition on the intestinal wall of rats by evaluating alterations in the rupture force and dosing tissue collagen in the ileum and distal colon. METHODS: One hundred and twenty rats, that had an average weight of 100g, were used. They received water and a standard diet with 20% protein during 7 days for adaptation to the diet itself and to environmental conditions. After that period, the animals were randomly distributed in two groups of 60 rats each: Group 1 - the animals received a control diet with 20% casein for 21 days; Group 2 - hypoprotein diet with 2% casein for 21 days. After the adaptation period, 12 animals of each group were sacrificed at 5 moments: the beginning of experimental period (M0), 4° day (M1), 7° day (M2), 14° day (M3) and 21° day (M4). The diet to the other rats was maintained until the last sacrifice. The following variables were evaluated: body weight, blood albumin rate, tissue's hydroxyproline, hydroxyproline/total protein ratio and rupture strength in the intestinal wall of the ileum and the distal colon. RESULTS: It was observed that the rupture strength in the ileum segment and distal colon was lower in malnourished animals (Group 2); the loss of mechanical resistance was higher in the distal colon segment than in the ileum probably due to the smaller concentration of tissue collagen in the distal colon. CONCLUSION: Protein malnutrition induces the loss of mechanical resistance of the ileum and distal colon and may be associated with a smaller percentage of collagenous tissue formation in the intestinal wall. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502008000500008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.23 n.5 2008 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126439162904576 |