Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats

Detalhes bibliográficos
Autor(a) principal: Grezzana Filho,Tomaz de Jesus Maria
Data de Publicação: 2009
Outros Autores: Mendonça,Tais Burmann de, Gabiatti,Gémerson, Kruel,Cleber Dario Pinto, Corso,Carlos Otávio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000400004
Resumo: PURPOSE: Evaluation of the Mean Arterial Pressure (MAP) and body temperature changes in a new model of liver ischemia-reperfusion applying topical Hypothermia and Ischemic Preconditioning (IPC). METHODS: Rats (n= 32) were divided in 5 groups: Control (C), Normothermic Ischemia (NI), Ischemic Preconditioning (IPC), Hypothermia 26°C plus IPC (H+IPC) and Hypothermia 26°C (H). MAP and body temperature were recorded at 30 minutes intervals throughout the entire experiment. The study groups underwent 90 minutes partial hepatic ischemia followed by 120 minutes of reperfusion. The median and lateral left lobes were isolated and topical 26°C hypothermia was induced by superfusion of cooled saline solution in H+IPC and H groups. A 10 minutes protocol of ischemia and reperfusion was applied in the IPC and H+IPC groups before the major ischemic insult. RESULTS: There was no significant difference in MAP and body temperature means between the groups throughout the experiments. CONCLUSION: The present model allows the induction of topical hepatic hypothermia associated or not to IPC. New studies to evaluate the possible synergistic effects of these tools can be reproduced without significant changes in macrohemodynamics and body temperature, or in other words, under stable conditions.
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spelling Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in ratsHypothermia, InducedIschemic PreconditioningRatsPURPOSE: Evaluation of the Mean Arterial Pressure (MAP) and body temperature changes in a new model of liver ischemia-reperfusion applying topical Hypothermia and Ischemic Preconditioning (IPC). METHODS: Rats (n= 32) were divided in 5 groups: Control (C), Normothermic Ischemia (NI), Ischemic Preconditioning (IPC), Hypothermia 26°C plus IPC (H+IPC) and Hypothermia 26°C (H). MAP and body temperature were recorded at 30 minutes intervals throughout the entire experiment. The study groups underwent 90 minutes partial hepatic ischemia followed by 120 minutes of reperfusion. The median and lateral left lobes were isolated and topical 26°C hypothermia was induced by superfusion of cooled saline solution in H+IPC and H groups. A 10 minutes protocol of ischemia and reperfusion was applied in the IPC and H+IPC groups before the major ischemic insult. RESULTS: There was no significant difference in MAP and body temperature means between the groups throughout the experiments. CONCLUSION: The present model allows the induction of topical hepatic hypothermia associated or not to IPC. New studies to evaluate the possible synergistic effects of these tools can be reproduced without significant changes in macrohemodynamics and body temperature, or in other words, under stable conditions.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000400004Acta Cirúrgica Brasileira v.24 n.4 2009reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502009000400004info:eu-repo/semantics/openAccessGrezzana Filho,Tomaz de Jesus MariaMendonça,Tais Burmann deGabiatti,GémersonKruel,Cleber Dario PintoCorso,Carlos Otávioeng2009-08-21T00:00:00Zoai:scielo:S0102-86502009000400004Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2009-08-21T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
title Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
spellingShingle Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
Grezzana Filho,Tomaz de Jesus Maria
Hypothermia, Induced
Ischemic Preconditioning
Rats
title_short Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
title_full Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
title_fullStr Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
title_full_unstemmed Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
title_sort Topic liver hypothermia and ischemic preconditioning: a new model of ischemia and reperfusion in rats
author Grezzana Filho,Tomaz de Jesus Maria
author_facet Grezzana Filho,Tomaz de Jesus Maria
Mendonça,Tais Burmann de
Gabiatti,Gémerson
Kruel,Cleber Dario Pinto
Corso,Carlos Otávio
author_role author
author2 Mendonça,Tais Burmann de
Gabiatti,Gémerson
Kruel,Cleber Dario Pinto
Corso,Carlos Otávio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Grezzana Filho,Tomaz de Jesus Maria
Mendonça,Tais Burmann de
Gabiatti,Gémerson
Kruel,Cleber Dario Pinto
Corso,Carlos Otávio
dc.subject.por.fl_str_mv Hypothermia, Induced
Ischemic Preconditioning
Rats
topic Hypothermia, Induced
Ischemic Preconditioning
Rats
description PURPOSE: Evaluation of the Mean Arterial Pressure (MAP) and body temperature changes in a new model of liver ischemia-reperfusion applying topical Hypothermia and Ischemic Preconditioning (IPC). METHODS: Rats (n= 32) were divided in 5 groups: Control (C), Normothermic Ischemia (NI), Ischemic Preconditioning (IPC), Hypothermia 26°C plus IPC (H+IPC) and Hypothermia 26°C (H). MAP and body temperature were recorded at 30 minutes intervals throughout the entire experiment. The study groups underwent 90 minutes partial hepatic ischemia followed by 120 minutes of reperfusion. The median and lateral left lobes were isolated and topical 26°C hypothermia was induced by superfusion of cooled saline solution in H+IPC and H groups. A 10 minutes protocol of ischemia and reperfusion was applied in the IPC and H+IPC groups before the major ischemic insult. RESULTS: There was no significant difference in MAP and body temperature means between the groups throughout the experiments. CONCLUSION: The present model allows the induction of topical hepatic hypothermia associated or not to IPC. New studies to evaluate the possible synergistic effects of these tools can be reproduced without significant changes in macrohemodynamics and body temperature, or in other words, under stable conditions.
publishDate 2009
dc.date.none.fl_str_mv 2009-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000400004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502009000400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.24 n.4 2009
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
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institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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