Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats

Detalhes bibliográficos
Autor(a) principal: Minossi,José Guilherme
Data de Publicação: 2014
Outros Autores: Lima,Flávio de Oliveira, Caramori,Carlos Antonio, Hasimoto,Claudia Nishida, Ortolan,Érica Veruska Paiva, Rodrigues,Paulo Antônio, Spadella,César Tadeu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118
Resumo: PURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.
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spelling Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in ratsDiabetes MellitusAlloxanTensile StrengthWound HealingHistologyRatsPURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118Acta Cirúrgica Brasileira v.29 n.2 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014000200008info:eu-repo/semantics/openAccessMinossi,José GuilhermeLima,Flávio de OliveiraCaramori,Carlos AntonioHasimoto,Claudia NishidaOrtolan,Érica Veruska PaivaRodrigues,Paulo AntônioSpadella,César Tadeueng2015-10-08T00:00:00Zoai:scielo:S0102-86502014000200118Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
title Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
spellingShingle Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
Minossi,José Guilherme
Diabetes Mellitus
Alloxan
Tensile Strength
Wound Healing
Histology
Rats
title_short Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
title_full Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
title_fullStr Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
title_full_unstemmed Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
title_sort Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
author Minossi,José Guilherme
author_facet Minossi,José Guilherme
Lima,Flávio de Oliveira
Caramori,Carlos Antonio
Hasimoto,Claudia Nishida
Ortolan,Érica Veruska Paiva
Rodrigues,Paulo Antônio
Spadella,César Tadeu
author_role author
author2 Lima,Flávio de Oliveira
Caramori,Carlos Antonio
Hasimoto,Claudia Nishida
Ortolan,Érica Veruska Paiva
Rodrigues,Paulo Antônio
Spadella,César Tadeu
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Minossi,José Guilherme
Lima,Flávio de Oliveira
Caramori,Carlos Antonio
Hasimoto,Claudia Nishida
Ortolan,Érica Veruska Paiva
Rodrigues,Paulo Antônio
Spadella,César Tadeu
dc.subject.por.fl_str_mv Diabetes Mellitus
Alloxan
Tensile Strength
Wound Healing
Histology
Rats
topic Diabetes Mellitus
Alloxan
Tensile Strength
Wound Healing
Histology
Rats
description PURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014000200008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 n.2 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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