Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118 |
Resumo: | PURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections. |
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Acta Cirúrgica Brasileira (Online) |
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Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in ratsDiabetes MellitusAlloxanTensile StrengthWound HealingHistologyRatsPURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118Acta Cirúrgica Brasileira v.29 n.2 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014000200008info:eu-repo/semantics/openAccessMinossi,José GuilhermeLima,Flávio de OliveiraCaramori,Carlos AntonioHasimoto,Claudia NishidaOrtolan,Érica Veruska PaivaRodrigues,Paulo AntônioSpadella,César Tadeueng2015-10-08T00:00:00Zoai:scielo:S0102-86502014000200118Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
title |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
spellingShingle |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats Minossi,José Guilherme Diabetes Mellitus Alloxan Tensile Strength Wound Healing Histology Rats |
title_short |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
title_full |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
title_fullStr |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
title_full_unstemmed |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
title_sort |
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats |
author |
Minossi,José Guilherme |
author_facet |
Minossi,José Guilherme Lima,Flávio de Oliveira Caramori,Carlos Antonio Hasimoto,Claudia Nishida Ortolan,Érica Veruska Paiva Rodrigues,Paulo Antônio Spadella,César Tadeu |
author_role |
author |
author2 |
Lima,Flávio de Oliveira Caramori,Carlos Antonio Hasimoto,Claudia Nishida Ortolan,Érica Veruska Paiva Rodrigues,Paulo Antônio Spadella,César Tadeu |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Minossi,José Guilherme Lima,Flávio de Oliveira Caramori,Carlos Antonio Hasimoto,Claudia Nishida Ortolan,Érica Veruska Paiva Rodrigues,Paulo Antônio Spadella,César Tadeu |
dc.subject.por.fl_str_mv |
Diabetes Mellitus Alloxan Tensile Strength Wound Healing Histology Rats |
topic |
Diabetes Mellitus Alloxan Tensile Strength Wound Healing Histology Rats |
description |
PURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long- laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200118 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502014000200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.29 n.2 2014 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126442231037952 |